Align diaminopimelate decarboxylase (EC 4.1.1.20) (characterized)
to candidate YP_001328203.1 Smed_2538 diaminopimelate decarboxylase
Query= BRENDA::Q9KVL7 (417 letters) >NCBI__GCF_000017145.1:YP_001328203.1 Length = 422 Score = 410 bits (1054), Expect = e-119 Identities = 214/419 (51%), Positives = 291/419 (69%), Gaps = 7/419 (1%) Query: 1 MDYFNYQEDGQLWAEQVPLADLANQYGTPLYVYSRATLERHWHAFDKSVGDYPHLICYAV 60 M++F Y+ DG L+AE VPL ++A GTP Y YS ATLERH+ F ++ D ++CYA+ Sbjct: 1 MNHFQYR-DGILYAEDVPLTEIARAVGTPFYCYSTATLERHYKVFSQAFADVDAMVCYAM 59 Query: 61 KANSNLGVLNTLARLGSGFDIVSVGELERVLAAGGDPSKVVFSGVGKTEAEMKRALQLKI 120 KANSN VL TL RLG+G D+VS GEL R LAAG PS+++FSGVGKT EM AL+ I Sbjct: 60 KANSNQAVLKTLGRLGAGLDVVSEGELRRALAAGIPPSRIMFSGVGKTAREMDFALEAGI 119 Query: 121 KCFNVESEPELQRLNKVAGELGVKAPISLRINPDVDAKTHPYISTGLRDNKFGITFDRAA 180 CFNVESEPEL+ LN+ G +AP+S RINPDVDA+TH ISTG ++NKFGI+++RA Sbjct: 120 YCFNVESEPELEVLNQRGVRAGKRAPVSFRINPDVDARTHAKISTGKKENKFGISWERAR 179 Query: 181 QVYRLAHSLPNLDVHGIDCHIGSQLTALAPFIDATDRLLALIDSLKAEGIHIRHLDVGGG 240 VY A +LP ++V GID HIGSQ+T L PF DA L L+D+L+++G I H+D+GGG Sbjct: 180 SVYAHAATLPGIEVTGIDMHIGSQITELQPFDDAFKLLRELVDTLRSDGHVIEHVDLGGG 239 Query: 241 LGVVYRDE--LPPQPSEYAKALLDRLERHRDLELIFEPGRAIAANAGVLVTKVEFLKHTE 298 LG+ YR++ PP P YA + ++L + D +++ EPGR I NAG+LVT+V ++K Sbjct: 240 LGIPYREDNNPPPLPDAYADVVKNQL-KGLDCKIVTEPGRLIVGNAGILVTEVVYVKDGG 298 Query: 299 HKNFAIIDAAMNDLIRPALYQAWQDIIPLRPRQGEAQTY--DLVGPVCETSDFLGKDRDL 356 K F I+D AMNDLIRP LY+A+ +I P+R A D+VGPVCET D+L DR++ Sbjct: 299 DKTFVIVDGAMNDLIRPTLYEAYHEIRPVRISAASAPRIKADVVGPVCETGDYLALDREM 358 Query: 357 VL-QEGDLLAVRSSGAYGFTMSSNYNTRPRVAEVMVDGNKTYLVRQREELSSLWALESV 414 + + GDLLA+ S+GAYG + YN+R V EV+V G++ +++R R++ L L+SV Sbjct: 359 AMPRAGDLLAIGSAGAYGAVQAGTYNSRLLVPEVLVKGDRFHVIRPRKDYDELIGLDSV 417 Lambda K H 0.319 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 466 Number of extensions: 24 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 417 Length of database: 422 Length adjustment: 32 Effective length of query: 385 Effective length of database: 390 Effective search space: 150150 Effective search space used: 150150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate YP_001328203.1 Smed_2538 (diaminopimelate decarboxylase)
to HMM TIGR01048 (lysA: diaminopimelate decarboxylase (EC 4.1.1.20))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01048.hmm # target sequence database: /tmp/gapView.8076.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01048 [M=417] Accession: TIGR01048 Description: lysA: diaminopimelate decarboxylase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.3e-148 480.9 0.0 1.4e-148 480.7 0.0 1.0 1 lcl|NCBI__GCF_000017145.1:YP_001328203.1 Smed_2538 diaminopimelate decarb Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000017145.1:YP_001328203.1 Smed_2538 diaminopimelate decarboxylase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 480.7 0.0 1.4e-148 1.4e-148 4 415 .. 6 414 .. 3 416 .. 0.97 Alignments for each domain: == domain 1 score: 480.7 bits; conditional E-value: 1.4e-148 TIGR01048 4 kkdgeleiegvdlkelaeefgtPlYvydeetlrerlealkeafkaeeslvlYAvKAnsnlavlrllaee 72 +dg l++e+v+l+e+a+ gtP+Y y+++tl+++++ +++af++ +++v+YA+KAnsn avl++l ++ lcl|NCBI__GCF_000017145.1:YP_001328203.1 6 YRDGILYAEDVPLTEIARAVGTPFYCYSTATLERHYKVFSQAFADVDAMVCYAMKANSNQAVLKTLGRL 74 57899****************************************999********************* PP TIGR01048 73 GlgldvvsgGEleralaAgvkaekivfsgngkseeeleaaleleiklinvdsveelelleeiakelgkk 141 G+gldvvs+GEl ralaAg+++++i+fsg+gk+ +e+ ale++i ++nv+s+ ele l++ ++gk+ lcl|NCBI__GCF_000017145.1:YP_001328203.1 75 GAGLDVVSEGELRRALAAGIPPSRIMFSGVGKTAREMDFALEAGIYCFNVESEPELEVLNQRGVRAGKR 143 ********************************************************************* PP TIGR01048 142 arvllRvnpdvdaktheyisTGlkesKFGieveeaeeayelalkleslelvGihvHIGSqildlepfve 210 a+v++R+npdvda+th +isTG+ke+KFGi+ e+a+++y a++l+ +e++Gi++HIGSqi++l+pf + lcl|NCBI__GCF_000017145.1:YP_001328203.1 144 APVSFRINPDVDARTHAKISTGKKENKFGISWERARSVYAHAATLPGIEVTGIDMHIGSQITELQPFDD 212 ********************************************************************* PP TIGR01048 211 aaekvvklleelkeegieleeldlGGGlgisyeeeeeapdl.eeyaeklleklekeaelglklklilEp 278 a++ + +l+++l+++g +e++dlGGGlgi+y+e++++p l + ya++++++l++ l+ k++ Ep lcl|NCBI__GCF_000017145.1:YP_001328203.1 213 AFKLLRELVDTLRSDGHVIEHVDLGGGLGIPYREDNNPPPLpDAYADVVKNQLKG-----LDCKIVTEP 276 **********************************999887615678888888877.....8******** PP TIGR01048 279 GRslvanagvlltrVesvKevesrkfvlvDagmndliRpalYeayheiaalkr.leeeetetvdvvGpl 346 GR +v+nag+l+t+V +vK+ + ++fv+vD++mndliRp lYeayhei++++ +++ ++dvvGp+ lcl|NCBI__GCF_000017145.1:YP_001328203.1 277 GRLIVGNAGILVTEVVYVKDGGDKTFVIVDGAMNDLIRPTLYEAYHEIRPVRIsAASAPRIKADVVGPV 345 **************************************************964356678899******* PP TIGR01048 347 CEsgDvlakdrelpeveeGdllavasaGAYgasmssnYnsrprpaevlveegkarlirrretledllal 415 CE+gD la dre+ ++Gdlla+ saGAYga + +Ynsr + evlv++++ ++ir r+ +++l+ l lcl|NCBI__GCF_000017145.1:YP_001328203.1 346 CETGDYLALDREMAMPRAGDLLAIGSAGAYGAVQAGTYNSRLLVPEVLVKGDRFHVIRPRKDYDELIGL 414 *****************************************************************9976 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (417 nodes) Target sequences: 1 (422 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 12.40 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory