Alignments for a candidate for hisN in Sinorhizobium fredii NGR234

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisN in Sinorhizobium fredii NGR234

Align Histidinol-phosphatase [alternative form] (EC 3.1.3.15) (characterized)
to candidate YP_002827372.1 NGR_c28730 inositol mono-phosphatase family protein

Query= reanno::Phaeo:GFF2154
         (250 letters)



>NCBI__GCF_000018545.1:YP_002827372.1
          Length = 258

 Score =  243 bits (621), Expect = 2e-69
 Identities = 126/247 (51%), Positives = 164/247 (66%), Gaps = 1/247 (0%)

Query: 1   MADAARQAILPYFRSAGLQSDNKLDEGFDPVTIADRAAEQAMRSVLSELRPEDSILGEEF 60
           +ADAA+   LP FR+ G    NKL+ GFDPVT AD++AE A+R ++ +  PE  ILGEE 
Sbjct: 11  LADAAKAETLPRFRT-GTSVVNKLEGGFDPVTEADQSAEAAIRRLIEDSFPEHGILGEEH 69

Query: 61  GETHGQSGRTWVLDPIDGTRGFISGTPTWGVLIALGDADGPFLGIVDQPYIGERFIGTPE 120
           G         WV+DPIDGTR FISG P WG LI L       +G++DQP+ GER+    +
Sbjct: 70  GNIGLDREHVWVIDPIDGTRAFISGLPVWGTLIGLYRNGKAIMGLMDQPFTGERYFADGQ 129

Query: 121 GASLTGPLGHSALVTRATDSLSEATLFTTFPEVGTEAERAAFQRVSAQVRLTRYGMDCYA 180
            +   GP G + L TRA D+LS+A LFTT P + T   +A F+ + A+VRL RYG DCYA
Sbjct: 130 KSIYRGPGGENVLSTRACDALSDAVLFTTSPHLYTGELKARFEALQAKVRLFRYGCDCYA 189

Query: 181 YALLAAGQCDLVIEAGLNAYDIQAPIAVIQAAGGVVTNWQGEPAHEGGQVLAAATAELHA 240
           +ALLAAG  DLV+E GL  YD+   I +I+ AGGVVT+WQG PA  GG+V+AA + ELHA
Sbjct: 190 FALLAAGHVDLVVECGLKPYDVGGLIPLIEQAGGVVTDWQGGPAEMGGEVIAAGSPELHA 249

Query: 241 AALALIQ 247
            AL +++
Sbjct: 250 QALQMLR 256


Lambda     K      H
   0.317    0.134    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 222
Number of extensions: 8
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 250
Length of database: 258
Length adjustment: 24
Effective length of query: 226
Effective length of database: 234
Effective search space:    52884
Effective search space used:    52884
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate YP_002827372.1 NGR_c28730 (inositol mono-phosphatase family protein)
to HMM TIGR02067 (hisN: histidinol-phosphatase (EC 3.1.3.15))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR02067.hmm
# target sequence database:        /tmp/gapView.26741.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02067  [M=252]
Accession:   TIGR02067
Description: his_9_HisN: histidinol-phosphatase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.7e-102  326.8   0.0   5.3e-102  326.6   0.0    1.0  1  lcl|NCBI__GCF_000018545.1:YP_002827372.1  NGR_c28730 inositol mono-phospha


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000018545.1:YP_002827372.1  NGR_c28730 inositol mono-phosphatase family protein
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  326.6   0.0  5.3e-102  5.3e-102       2     251 ..       5     255 ..       4     256 .. 0.98

  Alignments for each domain:
  == domain 1  score: 326.6 bits;  conditional E-value: 5.3e-102
                                 TIGR02067   2 lalalelaeaageailkyfrasdlkvdkksdk.tpVteADraaEeaireliaakfPddgilGEEfgeee 69 
                                               ++++++la+aa++++l++fr+ +++v+k +   +pVteAD++aE+air+li+++fP++gilGEE+g++ 
  lcl|NCBI__GCF_000018545.1:YP_002827372.1   5 RSFFDRLADAAKAETLPRFRTGTSVVNKLEGGfDPVTEADQSAEAAIRRLIEDSFPEHGILGEEHGNIG 73 
                                               67999************************9999************************************ PP

                                 TIGR02067  70 edaeyvWvlDPiDGTksFirGvPvwgtLiaLlekgkpvlGvisqPalgerfvaakgegallng.gerel 137
                                                d+e+vWv+DPiDGT++Fi+G+PvwgtLi+L+++gk+++G+++qP++ger++a+ ++  ++ + ge+ l
  lcl|NCBI__GCF_000018545.1:YP_002827372.1  74 LDREHVWVIDPIDGTRAFISGLPVWGTLIGLYRNGKAIMGLMDQPFTGERYFADGQKSIYRGPgGENVL 142
                                               **********************************************************9999867799* PP

                                 TIGR02067 138 rvsevaklsdAvlvttspkaledeeereafeklrrkarltryggdcyayalvAsGrvdlvveaelspyD 206
                                               +++++ +lsdAvl+ttsp +l + e +++fe+l++k+rl ryg+dcya+al+A+G+vdlvve++l+pyD
  lcl|NCBI__GCF_000018545.1:YP_002827372.1 143 STRACDALSDAVLFTTSP-HLYTGELKARFEALQAKVRLFRYGCDCYAFALLAAGHVDLVVECGLKPYD 210
                                               ******************.9************************************************* PP

                                 TIGR02067 207 iaalipiieeAggvitdwkGkeaeeggeavaaanaalhdevlell 251
                                               +  lip+ie+Aggv+tdw+G +ae+gge++aa++++lh+++l++l
  lcl|NCBI__GCF_000018545.1:YP_002827372.1 211 VGGLIPLIEQAGGVVTDWQGGPAEMGGEVIAAGSPELHAQALQML 255
                                               *******************************************98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (252 nodes)
Target sequences:                          1  (258 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.33
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis