Align 3-isopropylmalate dehydratase large subunit; EC 4.2.1.33 (characterized)
to candidate YP_002827628.1 NGR_c31400 3-isopropylmalate dehydratase large subunit
Query= CharProtDB::CH_024771 (466 letters) >NCBI__GCF_000018545.1:YP_002827628.1 Length = 469 Score = 632 bits (1629), Expect = 0.0 Identities = 311/465 (66%), Positives = 371/465 (79%), Gaps = 1/465 (0%) Query: 3 KTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFATMD 62 +TLY+K++D H+V + ++ T LLYIDRHLVHEVTSPQAF+GLR GR VR P KT A +D Sbjct: 5 RTLYDKIWDDHLVNQQDDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRKVRAPEKTLAVVD 64 Query: 63 HNVSTQT-KDINACGEMARIQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPG 121 HNV T + + E +RIQ++ L +N +FGVE Y N QGIVH++GPEQG TLPG Sbjct: 65 HNVPTSPDRHLGIKNEESRIQVEALARNAADFGVEYYSENDKRQGIVHIVGPEQGFTLPG 124 Query: 122 MTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAPGITAK 181 MTIVCGDSHT+THGAFGALA GIGTSEVEHVLATQTL Q +AK M + V G+ PG+TAK Sbjct: 125 MTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGQLPPGVTAK 184 Query: 182 DIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTFN 241 DI+LAIIG+ G+AGGTGHV+EF GEAIR LSMEGRMT+CNM IE GA+AGL+APDETTF+ Sbjct: 185 DIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTICNMTIEGGARAGLIAPDETTFD 244 Query: 242 YVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISVN 301 Y+K + APKGK +D A+ YWKTL TDEGA +D +V L A ++ P V+WG++P VISV Sbjct: 245 YIKDKPRAPKGKAWDMALDYWKTLHTDEGAHYDRIVVLDAADLPPIVSWGSSPEDVISVQ 304 Query: 302 DNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFIGSCTNSRIEDLRAAAEI 361 +P+P + +RAS +AL YMGLKPG +T++A+D+VFIGSCTN RIEDLRA A++ Sbjct: 305 GAVPNPDDIQEETKRASKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRAVAKV 364 Query: 362 AKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNPG 421 +GRKVA V A++VPGSG VK QAEAEGLDKIF EAGF+WR PGCSMCLAMN+DRL PG Sbjct: 365 VEGRKVASTVSAMIVPGSGLVKEQAEAEGLDKIFKEAGFDWREPGCSMCLAMNDDRLKPG 424 Query: 422 ERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNIK 466 ERCASTSNRNFEGRQG GRTHLVSPAMAAAAAV GHF DIR K Sbjct: 425 ERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAVAGHFVDIREWK 469 Lambda K H 0.317 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 794 Number of extensions: 27 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 466 Length of database: 469 Length adjustment: 33 Effective length of query: 433 Effective length of database: 436 Effective search space: 188788 Effective search space used: 188788 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate YP_002827628.1 NGR_c31400 (3-isopropylmalate dehydratase large subunit)
to HMM TIGR00170 (leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00170.hmm # target sequence database: /tmp/gapView.15997.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00170 [M=466] Accession: TIGR00170 Description: leuC: 3-isopropylmalate dehydratase, large subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.3e-251 821.0 0.1 1.5e-251 820.8 0.1 1.0 1 lcl|NCBI__GCF_000018545.1:YP_002827628.1 NGR_c31400 3-isopropylmalate deh Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000018545.1:YP_002827628.1 NGR_c31400 3-isopropylmalate dehydratase large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 820.8 0.1 1.5e-251 1.5e-251 2 465 .. 4 467 .. 3 468 .. 0.99 Alignments for each domain: == domain 1 score: 820.8 bits; conditional E-value: 1.5e-251 TIGR00170 2 aktlyeklfdahvvkeaenetdllyidrhlvhevtspqafeglraagrkvrrvdktlatldhniste.s 69 ++tly+k++d h+v++++++t llyidrhlvhevtspqafeglr agrkvr ++ktla +dhn++t lcl|NCBI__GCF_000018545.1:YP_002827628.1 4 PRTLYDKIWDDHLVNQQDDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRKVRAPEKTLAVVDHNVPTSpD 72 8*****************************************************************846 PP TIGR00170 70 rdveikeekaklqvkeleknvkefgvklfdlssaeqgivhvvgpeegltlpgktivcgdshtathgafg 138 r + ik+e++++qv++l +n+ +fgv+++ ++++qgivh+vgpe+g+tlpg+tivcgdsht+thgafg lcl|NCBI__GCF_000018545.1:YP_002827628.1 73 RHLGIKNEESRIQVEALARNAADFGVEYYSENDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFG 141 89******************************************************************* PP TIGR00170 139 alafgigtsevehvlatqtlkqaraktlkievegklakgitakdiilaiigkigvaggtgyvvefagea 207 ala gigtsevehvlatqtl+q++ak++ ++v+g+l +g+takdiilaiig ig+aggtg+v+efagea lcl|NCBI__GCF_000018545.1:YP_002827628.1 142 ALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGQLPPGVTAKDIILAIIGEIGTAGGTGHVIEFAGEA 210 ********************************************************************* PP TIGR00170 208 irdlsmeermtvcnmaieagakagliapdettfeyvkdrkyapkgkefekavaywktlktdegakfdkv 276 ir+lsme+rmt+cnm+ie ga+agliapdettf+y+kd++ apkgk ++ a++ywktl+tdega++d+ lcl|NCBI__GCF_000018545.1:YP_002827628.1 211 IRSLSMEGRMTICNMTIEGGARAGLIAPDETTFDYIKDKPRAPKGKAWDMALDYWKTLHTDEGAHYDRI 279 ********************************************************************* PP TIGR00170 277 vtleakdispqvtwgtnpgqvlsvneevpdpksladpvekasaekalaylglepgtklkdikvdkvfig 345 v+l+a d+ p v+wg +p++v+sv++ vp+p+++ ++ ++as +al+y+gl+pgtk++di+vd+vfig lcl|NCBI__GCF_000018545.1:YP_002827628.1 280 VVLDAADLPPIVSWGSSPEDVISVQGAVPNPDDIQEETKRASKWRALDYMGLKPGTKITDIAVDRVFIG 348 ********************************************************************* PP TIGR00170 346 sctnsriedlraaaevvkgkkvadnvklalvvpgsglvkkqaekegldkifleagfewreagcslclgm 414 sctn+riedlra a+vv g+kva++v a++vpgsglvk+qae+egldkif eagf+wre+gcs+cl+m lcl|NCBI__GCF_000018545.1:YP_002827628.1 349 SCTNGRIEDLRAVAKVVEGRKVASTVS-AMIVPGSGLVKEQAEAEGLDKIFKEAGFDWREPGCSMCLAM 416 **************************9.***************************************** PP TIGR00170 415 nndvldeyercastsnrnfegrqgkgarthlvspamaaaaavagkfvdire 465 n+d+l+++ercastsnrnfegrqg ++rthlvspamaaaaavag+fvdire lcl|NCBI__GCF_000018545.1:YP_002827628.1 417 NDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAVAGHFVDIRE 467 **************************************************8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (466 nodes) Target sequences: 1 (469 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01 # Mc/sec: 11.33 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory