Finding step glyXL for glycine biosynthesis in Bacteroides thetaiotaomicron VPI-5482
No candidates for glyXL: putative glycine synthesis enzyme, catalytic component
GapMind classifies a step as low confidence even if it does not find any candidates. You can still try to find candidates by using Curated BLAST (which searches the 6-frame translation) or by text search of the annotations (which may indicate weak homology, under 30% identity or 50% coverage, that GapMind does not consider). See the links below.
Definition of step glyXL
- UniProt sequence Q8G510: RecName: Full=UPF0210 protein BL1209 {ECO:0000255|HAMAP-Rule:MF_01221};
- UniProt sequence Q6LXC5: RecName: Full=UPF0210 protein MMP1427 {ECO:0000255|HAMAP-Rule:MF_01221};
- UniProt sequence Q8DRD2: RecName: Full=UPF0210 protein spr0218 {ECO:0000255|HAMAP-Rule:MF_01221};
- Comment: Glycine synthesis by Bifidobacterium breve or Methanococcus maripaludis requires two genes: a putative enzyme (distantly related to anaerobic ribonucleotide reductase), which we call glyXL, and an ACT domain protein, which we call glyXS (BBR_RS12920 and BBR_RS12915 or MMP_RS07345 and MMP_RS03450, Anthony Shiver and Leslie Day). These proteins are usually encoded next to each other (although not in Methanococcus). A homolog of glyXL from Streptococcus (spr0218, Q8DRD2) is also required for glycine synthesis (PMC2739083). Also, glyXS:glyXL is regulated by a glycine riboswitch in Bacillus methanolicus (see BMMGA3_03000 in PMC4342826).
Or cluster all characterized glyXL proteins
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory