Finding step serC for L-cysteine biosynthesis in Azospirillum brasilense Sp245
No candidates for serC: 3-phosphoserine aminotransferase
GapMind classifies a step as low confidence even if it does not find any candidates. You can still try to find candidates by using Curated BLAST (which searches the 6-frame translation) or by text search of the annotations (which may indicate weak homology, under 30% identity or 50% coverage, that GapMind does not consider). See the links below.
Definition of step serC
- Curated proteins or TIGRFams with EC 2.6.1.52 (search)
- UniProt sequence Y959_METJA: RecName: Full=Uncharacterized aminotransferase MJ0959; EC=2.6.1.-;
- UniProt sequence Q2FXK2: RecName: Full=Aminotransferase class V domain-containing protein {ECO:0000259|Pfam:PF00266};
- Predicted: UniProt sequence A5I0W7: SubName: Full=Serine--glyoxylate aminotransferase {ECO:0000313|EMBL:CAL82678.1}; EC=2.6.1.45 {ECO:0000313|EMBL:CAL82678.1};
- Predicted: UniProt sequence A0A843E9R6: SubName: Full=Alanine--glyoxylate aminotransferase family protein {ECO:0000313|EMBL:MBO5653953.1};
- Predicted: UniProt sequence A0A1T4W7T3: SubName: Full=Aspartate aminotransferase {ECO:0000313|EMBL:SKA73374.1};
- Predicted: UniProt sequence A0A097AUI2: SubName: Full=Serine-pyruvate aminotransferase {ECO:0000313|EMBL:AIS53461.1}; EC=2.6.1.51 {ECO:0000313|EMBL:AIS53461.1};
- Comment: MJ0959 (Y959_METJA) is phosphoserine transaminase (PMID:17071763). A mutant in the putative aminotransferase SAUSA300_1669 = Q2FXK2 is a serine auxotroph (PMC5912478), and this gene is conserved near other serine synthesis genes (SerC2 in PMC9026213). A5I0W7 is a putative aminotransferase and is conserved near serine synthesis genes (SerC3 in PMC9026213; also see CDIF630erm_01130 in PMC6110889). Some moderately diverged SerC3 homologs are also conserved near serine synthesis genes (A3204_00420, similar to A0A843E9R6; G452_RS0102660, similar to A0A1T4W7T3). In Thermoanaerobacter kivui LKT-1, a putative transaminase (TKV_RS11400, A0A097AUI2) is encoded next to serA; by homology, it might be a serine:pyruvate aminotransferase or a phosphoserine transaminase; given the conserved proximity to serA, we predicted that it is serC.
Or cluster all characterized serC proteins
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory