prs, hisG, hisI, hisE, hisA, hisF, hisH, hisB, hisC, hisN?, hisD
Overview: Histidine biosynthesis in GapMind is based on the MetaCyc pathway (link).
Or see definitions of steps
| Step | Description | Best candidate | 2nd candidate | Known gap? |
|---|---|---|---|---|
| prs | ribose-phosphate diphosphokinase | PERMA_RS06390 | ||
| hisG | ATP phosphoribosyltransferase | PERMA_RS06905 | ||
| hisI | phosphoribosyl-ATP pyrophosphatase | PERMA_RS01820 | PERMA_RS09180 | |
| hisE | phosphoribosyl-AMP cyclohydrolase | PERMA_RS01820 | PERMA_RS09180 | |
| hisA | 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase | PERMA_RS08165 | PERMA_RS04305 | |
| hisF | imidazole glycerol phosphate synthase, cyclase subunit | PERMA_RS04305 | PERMA_RS08165 | |
| hisH | imidazole glycerol phosphate synthase, amidotransferase subunit | PERMA_RS06585 | ||
| hisB | imidazoleglycerol-phosphate dehydratase | PERMA_RS02725 | ||
| hisC | histidinol-phosphate aminotransferase | PERMA_RS05565 | PERMA_RS09570 | |
| hisN? | histidinol-phosphate phosphatase | PERMA_RS02725 | PERMA_RS01120 | known gap |
| hisD | histidinal/histidinol dehydrogenase | PERMA_RS09180 |
Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory