Alignments for a candidate for ilvC in Methanosarcina acetivorans C2A

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvC in Methanosarcina acetivorans C2A

Align ketol-acid reductoisomerase (NAD+) (EC 1.1.1.382); ketol-acid reductoisomerase (NADP+) (EC 1.1.1.86) (characterized)
to candidate WP_011023690.1 MA_RS19745 ketol-acid reductoisomerase

Query= BRENDA::C8WR67
         (344 letters)



>NCBI__GCF_000007345.1:WP_011023690.1
          Length = 335

 Score =  440 bits (1132), Expect = e-128
 Identities = 218/335 (65%), Positives = 266/335 (79%), Gaps = 1/335 (0%)

Query: 1   MEKIYYDADISIQPLADKRIAVIGYGSQGHAHAQNLRDSGFDVVIGLRPGSS-WAKAEAD 59
           M  I YD + +   L DK IA++GYGSQGHAHA+NL +SG +VV+GLR GSS WAKAE+D
Sbjct: 1   MATIIYDNETTFDALKDKTIAIMGYGSQGHAHARNLHESGLNVVVGLRKGSSSWAKAESD 60

Query: 60  GFRVMAVGEAVEESDVIMILLPDERQPAVYEREIRPYLTAGKALAFAHGFNIHFSQIQPP 119
           G +VM V EA + +DVIMILLPDE+Q +VY  +I P L AG AL FAHGFNIH++QI PP
Sbjct: 61  GLKVMTVEEAAKAADVIMILLPDEKQASVYYSQIEPSLEAGNALVFAHGFNIHYNQIVPP 120

Query: 120 KDVDVFMVAPKGPGHLVRRVYEAGGGVPALIAVHQDASGQAKDLALAYARGIGAGRAGIL 179
           KDVDVFMVAPKGPGH+VRR Y  G GVP LIAV+QDA+G+A+DLALAYA+GIGA RAG+ 
Sbjct: 121 KDVDVFMVAPKGPGHIVRRTYTEGIGVPGLIAVYQDATGKARDLALAYAKGIGATRAGVY 180

Query: 180 TTTFREETETDLFGEQAVLCGGLSALIKAGFETLVEAGYQPEIAYFECLHEMKLIVDLIY 239
            TTFREETETDLFGEQ  LCGGLSALIK  FE LVEAGYQPE+AYFE  HE+KLIVDLIY
Sbjct: 181 ETTFREETETDLFGEQVDLCGGLSALIKTAFEVLVEAGYQPEMAYFETCHEVKLIVDLIY 240

Query: 240 EGGLEYMRYSISDTAQWGDFTSGPRIINEETKKEMRRILADIQSGAFAKSWILENQANRP 299
           EGGLE M +S+S+TA++G  T GPRIINEE+++ M   LA IQ+G FAK ++LE   N P
Sbjct: 241 EGGLERMWHSVSNTAEYGGMTVGPRIINEESREAMYEALARIQNGEFAKEFVLEGMVNHP 300

Query: 300 MFNAINRRELEHPIEVVGRKLRSMMPFIKAKRPGD 334
           +  A+ R+E EHP+EVVG+++R+ +P++  K   D
Sbjct: 301 VLKAMERQEKEHPLEVVGKEIRANIPWLNKKIDDD 335


Lambda     K      H
   0.320    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 375
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 344
Length of database: 335
Length adjustment: 28
Effective length of query: 316
Effective length of database: 307
Effective search space:    97012
Effective search space used:    97012
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_011023690.1 MA_RS19745 (ketol-acid reductoisomerase)
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00465.hmm
# target sequence database:        /tmp/gapView.5789.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00465  [M=314]
Accession:   TIGR00465
Description: ilvC: ketol-acid reductoisomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.2e-149  483.5   0.4   1.4e-149  483.3   0.4    1.0  1  lcl|NCBI__GCF_000007345.1:WP_011023690.1  MA_RS19745 ketol-acid reductoiso


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000007345.1:WP_011023690.1  MA_RS19745 ketol-acid reductoisomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  483.3   0.4  1.4e-149  1.4e-149       1     312 [.      15     327 ..      15     329 .. 0.99

  Alignments for each domain:
  == domain 1  score: 483.3 bits;  conditional E-value: 1.4e-149
                                 TIGR00465   1 lkgkkvaiiGyGsqGeaqalnlrdsglnvivglrkeaaswkkAeedGfkvltveeaikkadlimiLlpD 69 
                                               lk+k++ai+GyGsqG+a+a+nl++sglnv+vglrk+++sw+kAe dG+kv+tveea+k+ad+imiLlpD
  lcl|NCBI__GCF_000007345.1:WP_011023690.1  15 LKDKTIAIMGYGSQGHAHARNLHESGLNVVVGLRKGSSSWAKAESDGLKVMTVEEAAKAADVIMILLPD 83 
                                               79******************************************************************* PP

                                 TIGR00465  70 evqkevyeaeikpllkegkallfsHGfnivfkqivipkdvdvvlvAPKgpGalvReeykegrGvpsliA 138
                                               e q +vy  +i+p l++g+al+f+HGfni+++qiv+pkdvdv++vAPKgpG+ vR++y eg Gvp+liA
  lcl|NCBI__GCF_000007345.1:WP_011023690.1  84 EKQASVYYSQIEPSLEAGNALVFAHGFNIHYNQIVPPKDVDVFMVAPKGPGHIVRRTYTEGIGVPGLIA 152
                                               ********************************************************************* PP

                                 TIGR00465 139 veqdvtgeakeiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLveaGyqp 207
                                               v+qd+tg+a++ AlayAk+iG++ragv+ettF+eE+e+DLfGEq+ LcGgl+alik+af++LveaGyqp
  lcl|NCBI__GCF_000007345.1:WP_011023690.1 153 VYQDATGKARDLALAYAKGIGATRAGVYETTFREETETDLFGEQVDLCGGLSALIKTAFEVLVEAGYQP 221
                                               ********************************************************************* PP

                                 TIGR00465 208 elAyfeivhelklivdllkekGlelmrdavsntAklgalelr.eilkeelkkemqkilkeiqnGefake 275
                                               e+Ayfe++he+klivdl++e+Gle m+++vsntA++g+++++ +i++ee +++m++ l  iqnGefake
  lcl|NCBI__GCF_000007345.1:WP_011023690.1 222 EMAYFETCHEVKLIVDLIYEGGLERMWHSVSNTAEYGGMTVGpRIINEESREAMYEALARIQNGEFAKE 290
                                               ********************************************************************* PP

                                 TIGR00465 276 walekeagkpafeearkkekeqeiekvGkelralvka 312
                                               ++le ++++p +++++++eke+ +e vGke+ra +++
  lcl|NCBI__GCF_000007345.1:WP_011023690.1 291 FVLEGMVNHPVLKAMERQEKEHPLEVVGKEIRANIPW 327
                                               *********************************9887 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (314 nodes)
Target sequences:                          1  (335 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 8.75
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis