Alignments for a candidate for ilvD in Methanosarcina acetivorans C2A

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Methanosarcina acetivorans C2A

Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate WP_011021805.1 MA_RS09370 dihydroxy-acid dehydratase

Query= metacyc::MONOMER-11919
         (549 letters)



>NCBI__GCF_000007345.1:WP_011021805.1
          Length = 553

 Score =  660 bits (1704), Expect = 0.0
 Identities = 322/553 (58%), Positives = 426/553 (77%), Gaps = 6/553 (1%)

Query: 1   MKSDTIKRGIQRAPHRSLLARCGLTDDDFEKPFIGIANSYTDIVPGHIHLRELAEAVKEG 60
           M+S  IK G +RA +RSLL   G+TD + +KPFI + NS+ +IVPGHIHL +LAEAVK G
Sbjct: 1   MRSAIIKEGPERAANRSLLKATGVTDSEMKKPFIAVVNSWNEIVPGHIHLDKLAEAVKAG 60

Query: 61  VNAAGGVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCD 120
           +  AGGV FEF+T+ +CDG+AM H+GMKYSL SRE++ DT+E M  AH  DG+VL+PTCD
Sbjct: 61  IRNAGGVPFEFHTIGVCDGLAMGHEGMKYSLPSREVIEDTIELMVRAHQFDGMVLIPTCD 120

Query: 121 KIVPGMLMAAARLDIPAIVVTGGPMLPGEFKGRKVDLINVYEGVGTVSAGEMSEDELEEL 180
           KIVPG LMAA RLDIPAIVVTGGPMLPG    +  DLI+V+EGVG  SAG++SE EL+ L
Sbjct: 121 KIVPGHLMAAGRLDIPAIVVTGGPMLPGYVDDKYTDLISVFEGVGAYSAGKLSEAELKRL 180

Query: 181 ERCACPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMVQ 240
           E  +C G  SCAG+FTANTMAC+TEALG+SLPGCATAHAV ++K +IA+ SG+RIV +V+
Sbjct: 181 ENLSCAGAGSCAGMFTANTMACMTEALGLSLPGCATAHAVDAKKVRIAKESGERIVALVK 240

Query: 241 ENLKPTMIMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDELSRVIPH 300
           ENL P  I++Q++FENA+MVD+A+GGSTNTTLH+PA+A E  GL + L  FDELSR  PH
Sbjct: 241 ENLTPRKIVTQKSFENAIMVDMAVGGSTNTTLHLPALAHEF-GLELPLKTFDELSRTTPH 299

Query: 301 IASISPAGEHMMLDLDRAGGIPAVLKTLEDHINRECVTCTGRTVQENIENVKVG----HR 356
           + S+ P G + ML  DRAGG+ AV++ L   ++ + +T  G+T+ EN++ +++     + 
Sbjct: 300 LISLRPGGPNFMLHFDRAGGVEAVVQRLASKLHLDQLTVNGKTIGENLDELEIVNPKLNA 359

Query: 357 DVIRPLDSPVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSEDECMEAIF 416
           ++I  L++P+H+EGG+A+L+G+LAP GSVVKQ AV   M VH GPAKV++ E++ M++I 
Sbjct: 360 EIITTLENPIHAEGGIAVLKGSLAPDGSVVKQAAVDPKMRVHTGPAKVYDCEEDAMKSIL 419

Query: 417 GGRIDEGDVIVIRYEGPKGGPGMREMLNPTSAIAGMG-LERVALITDGRFSGGTRGPCVG 475
            G +  GD++VIRYEGPKGGPGMREML  T+AI GMG LE VALITDGRFSGGTRGPC+G
Sbjct: 420 AGDVKPGDIVVIRYEGPKGGPGMREMLAATAAIGGMGLLESVALITDGRFSGGTRGPCIG 479

Query: 476 HVSPEAMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREIEERLQSAVKPRRSVKGWLARY 535
           HVSPEA E GP+  V DGD+I I+IP R L + ++  E+E+R  + V P++ V G+LARY
Sbjct: 480 HVSPEASEGGPIGLVKDGDLIEINIPERILNLKVTEEELEKRKAAFVPPKKEVTGYLARY 539

Query: 536 RKLAGSADTGAVL 548
           ++   SA+TG ++
Sbjct: 540 QRSVHSANTGGIV 552


Lambda     K      H
   0.319    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1070
Number of extensions: 57
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 549
Length of database: 553
Length adjustment: 36
Effective length of query: 513
Effective length of database: 517
Effective search space:   265221
Effective search space used:   265221
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

Align candidate WP_011021805.1 MA_RS09370 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00110.hmm
# target sequence database:        /tmp/gapView.1323.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00110  [M=543]
Accession:   TIGR00110
Description: ilvD: dihydroxy-acid dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   9.6e-245  799.1   3.9   1.1e-244  798.9   3.9    1.0  1  lcl|NCBI__GCF_000007345.1:WP_011021805.1  MA_RS09370 dihydroxy-acid dehydr


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000007345.1:WP_011021805.1  MA_RS09370 dihydroxy-acid dehydratase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  798.9   3.9  1.1e-244  1.1e-244       1     542 [.      14     553 .]      14     553 .] 0.99

  Alignments for each domain:
  == domain 1  score: 798.9 bits;  conditional E-value: 1.1e-244
                                 TIGR00110   1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 
                                               a+r+llkatG++d +++kP+iavvns++eivPgh+hl++la++vk++i++aGgv++ef ti+v+DG+am
  lcl|NCBI__GCF_000007345.1:WP_011021805.1  14 ANRSLLKATGVTDSEMKKPFIAVVNSWNEIVPGHIHLDKLAEAVKAGIRNAGGVPFEFHTIGVCDGLAM 82 
                                               68******************************************************************* PP

                                 TIGR00110  70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138
                                               gheGmkysLpsre+i D++e +v+ah++D++v+i++CDkivPG lmaa rl+iPaivv+GGpm +g + 
  lcl|NCBI__GCF_000007345.1:WP_011021805.1  83 GHEGMKYSLPSREVIEDTIELMVRAHQFDGMVLIPTCDKIVPGHLMAAGRLDIPAIVVTGGPMLPGYVD 151
                                               ********************************************************************9 PP

                                 TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207
                                                 +  dl++vfe+vg+y+agklse+el+++e+ +c +agsC+G+ftan+mac+tealGlslPg++t++a
  lcl|NCBI__GCF_000007345.1:WP_011021805.1 152 -DKYTDLISVFEGVGAYSAGKLSEAELKRLENLSCAGAGSCAGMFTANTMACMTEALGLSLPGCATAHA 219
                                               .7999**************************************************************** PP

                                 TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsld 276
                                               ++a+k ++ak+sg+riv lvk+n++Pr+i+t+++fenai++d+a+GGstnt+Lhl+a+a+e g++l l+
  lcl|NCBI__GCF_000007345.1:WP_011021805.1 220 VDAKKVRIAKESGERIVALVKENLTPRKIVTQKSFENAIMVDMAVGGSTNTTLHLPALAHEFGLELPLK 288
                                               ********************************************************************* PP

                                 TIGR00110 277 dfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlr. 344
                                                fd+lsr++P+l +l+P+g +++   +raGGv+av++ l  + +lh d ltv Gkt++e+l++ ++ + 
  lcl|NCBI__GCF_000007345.1:WP_011021805.1 289 TFDELSRTTPHLISLRPGGPNFMLHFDRAGGVEAVVQRL--ASKLHLDQLTVNGKTIGENLDELEIVNp 355
                                               ***************************************..99********************998864 PP

                                 TIGR00110 345 .vdqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvk 412
                                                ++ ++i +l+np+++egg+avLkG+la++G+vvk+a+v+ ++ +++Gpakv++ ee+a+++il+g+vk
  lcl|NCBI__GCF_000007345.1:WP_011021805.1 356 kLNAEIITTLENPIHAEGGIAVLKGSLAPDGSVVKQAAVDPKMRVHTGPAKVYDCEEDAMKSILAGDVK 424
                                               48899**************************************************************** PP

                                 TIGR00110 413 eGdvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaial 481
                                                Gd+vviryeGPkGgPGmremLa t+a+ g+GL ++vaLitDGrfsGgtrG++iGhvsPea egG+i+l
  lcl|NCBI__GCF_000007345.1:WP_011021805.1 425 PGDIVVIRYEGPKGGPGMREMLAATAAIGGMGLLESVALITDGRFSGGTRGPCIGHVSPEASEGGPIGL 493
                                               ********************************************************************* PP

                                 TIGR00110 482 vedGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavld 542
                                               v+dGD i+i+i++r l+l+v eeel++r+a+ ++++ +ev g+La+y++ v sa++G+++d
  lcl|NCBI__GCF_000007345.1:WP_011021805.1 494 VKDGDLIEINIPERILNLKVTEEELEKRKAAFVPPK-KEVTGYLARYQRSVHSANTGGIVD 553
                                               *******************************99999.99******************9975 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (543 nodes)
Target sequences:                          1  (553 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02
# Mc/sec: 10.19
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis