Alignments for a candidate for aspS2 in Methylococcus capsulatus Bath

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Methylococcus capsulatus Bath

Align Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 (characterized)
to candidate WP_010961029.1 MCA_RS08730 aspartate--tRNA ligase

Query= SwissProt::Q51422
         (591 letters)



>NCBI__GCF_000008325.1:WP_010961029.1
          Length = 599

 Score =  797 bits (2059), Expect = 0.0
 Identities = 384/592 (64%), Positives = 471/592 (79%), Gaps = 2/592 (0%)

Query: 2   MRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAKA 61
           MRSH CG++NESL GQEV LCGWVHRRRDHGGVIF+D+RDREGL QVVFDPD  E F+ A
Sbjct: 1   MRSHRCGEVNESLLGQEVGLCGWVHRRRDHGGVIFVDLRDREGLVQVVFDPDHPEPFSLA 60

Query: 62  DRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELEVLNQAETPPFPLDEYSDVGE 121
           + VRSE+V+++ G+VR RPEG  NPN+ SG +EVL   LE+LN++ETPPFP++   +V E
Sbjct: 61  ETVRSEYVIRVVGQVRARPEGTENPNLKSGRVEVLATSLEILNRSETPPFPIESDIEVNE 120

Query: 122 ETRLRYRFIDLRRPEMAAKLKLRARITSSIRRYLDDNGFLDVETPILGRPTPEGARDYLV 181
           E RLRYR+IDLRRP M  +++LR  IT  +R +LD +GF ++ETP L + TPEGARDYLV
Sbjct: 121 EMRLRYRYIDLRRPVMQQRMRLRRDITRFLRNFLDHHGFYEIETPFLTKATPEGARDYLV 180

Query: 182 PSRTYPGHFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSF 241
           PSRT+P  FFALPQSPQL+KQLLM++G DRYYQ+ +CFRDEDLRADRQPEFTQ+DIETSF
Sbjct: 181 PSRTHPHSFFALPQSPQLYKQLLMISGMDRYYQVVRCFRDEDLRADRQPEFTQLDIETSF 240

Query: 242 LDESDIIGITEKMVRQLFKEVLDVEF-DEFPHMPFEEAMRRYGSDKPDLRIPLELVDVAD 300
           ++E  I+ + E M+R LF+E L+ E  D FP M + EAMRRY SDKPDLRIPLELVD+AD
Sbjct: 241 MNEDQIMTLMEAMIRDLFRETLNEELPDPFPRMTYAEAMRRYASDKPDLRIPLELVDIAD 300

Query: 301 QLKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNER 360
            +  VEFKVF+GPA DP+GRV AL++PG   + R  IDD T+FVGIYGAKGLAY+KVN+ 
Sbjct: 301 LMAGVEFKVFAGPAADPEGRVVALKLPGGGDLSRKDIDDLTRFVGIYGAKGLAYVKVNDL 360

Query: 361 AKGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADKAKIVCDALGALRIKVGHDL 420
             G+EGLQSPI+KF+PE+ +  IL+R  A  GD++FFGADKA+IV +++GALR+K+G D 
Sbjct: 361 GAGLEGLQSPILKFMPESTIRGILERTEAQTGDLIFFGADKARIVNESMGALRVKLGQDR 420

Query: 421 KLLTREWAPMWVVDFPMFE-ENDDGSLSALHHPFTSPKCTPAELEANPGAALSRAYDMVL 479
            L+ + W P+WV DFPMFE +   G   ALHHPFT+PKC+  +L  NPG ALSRAYDMVL
Sbjct: 421 GLVEKGWRPLWVTDFPMFEWDEKSGRWVALHHPFTAPKCSEEQLRQNPGRALSRAYDMVL 480

Query: 480 NGTELGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRL 539
           NGTE+GGGS+RIH   MQQ VF +LGI E E ++KFGFLL+AL+YG PPHGGLAFGLDRL
Sbjct: 481 NGTEIGGGSVRIHRTEMQQTVFDLLGIGEEEAQQKFGFLLNALRYGCPPHGGLAFGLDRL 540

Query: 540 VMLMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLREQPKAE 591
           VMLM+GASSIREV+AFPKTQSA   +  AP  V+   L EL IRLR+ P  E
Sbjct: 541 VMLMSGASSIREVMAFPKTQSAWCPLIDAPAQVNDAQLHELGIRLRKNPAVE 592


Lambda     K      H
   0.321    0.140    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1166
Number of extensions: 45
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 591
Length of database: 599
Length adjustment: 37
Effective length of query: 554
Effective length of database: 562
Effective search space:   311348
Effective search space used:   311348
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis