Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate WP_010959941.1 MCA_RS02960 threonine synthase
Query= BRENDA::O66740 (352 letters) >NCBI__GCF_000008325.1:WP_010959941.1 Length = 380 Score = 402 bits (1034), Expect = e-117 Identities = 196/371 (52%), Positives = 268/371 (72%), Gaps = 23/371 (6%) Query: 3 RWQGIIKQYKKYLPVDENTPIVTLYEGNTPLIEADNLARAIGFKGKIYLKYEGLNPTGSF 62 R+ G+I++Y+ LPV T I++L EG+TPLI +N+ R +G + ++Y+KYEGLNPTGSF Sbjct: 6 RYTGLIERYRDRLPVSAETRIISLGEGDTPLIRLENIPRLLGRRVELYVKYEGLNPTGSF 65 Query: 63 KDRGMTLAISKAVEAGKRAVICASTGNTSASAAAYAARAGLRAYVLLPKGAVAIGKLSQA 122 KDRGMT+A+++AVE G RA+ICASTGNTSA+AAAYAARAG+ A+VL+P G +A+GKL+QA Sbjct: 66 KDRGMTMAVTRAVEEGSRAIICASTGNTSAAAAAYAARAGITAFVLIPDGKIAMGKLAQA 125 Query: 123 MIYGAKVLAIQGTFDDALNIVRKIGENFPVEIVNSVNPYRIEGQKTAAFEICDTLGEAPD 182 M+YG+ V+ I+G FDD + +V+++ PV IVNS+NPYR++GQKTAAFEI D LG APD Sbjct: 126 MMYGSVVIQIKGNFDDGMRLVKEVAGEAPVTIVNSINPYRLQGQKTAAFEIVDELGRAPD 185 Query: 183 YHFIPVGNAGNITAYWKGFKIYYEEGK----------------------ITKLPRMMGWQ 220 YH +PVGNAGNITA+W G+ Y G+ T P+M+G+Q Sbjct: 186 YHCLPVGNAGNITAHWIGYSEYACGGRDCVTEACAFCNGECRFVSGGPVSTSRPKMVGYQ 245 Query: 221 AEGAAPIVKGYPIKNPQTIATAIKIGNPYSWKSALKAAQESGGKIDAVSDSEILYAYKLI 280 A G+AP ++G+ + +P+T+ATAI+IG+P SW+ A + ++ESGG D SD EIL A KL+ Sbjct: 246 ASGSAPFLRGHMVDHPETVATAIRIGHPQSWEQAWQVSRESGGWFDECSDEEILAAQKLL 305 Query: 281 ASTEGVFCEPASAASVAGLIKLVREGFFKGGEVVTCTLTGNGLKDPDTAIKVC-EEPITV 339 EGVFCEPASA S+AG ++ +R G G V TLTGNGLKDPDTAI C ++P+T+ Sbjct: 306 TEREGVFCEPASATSLAGALRDIRSGKIPEGSTVVLTLTGNGLKDPDTAIAQCTQKPVTI 365 Query: 340 PPDFDEVVKVL 350 + D V + + Sbjct: 366 EAELDSVKRAI 376 Lambda K H 0.317 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 427 Number of extensions: 19 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 352 Length of database: 380 Length adjustment: 30 Effective length of query: 322 Effective length of database: 350 Effective search space: 112700 Effective search space used: 112700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 49 (23.5 bits)
Align candidate WP_010959941.1 MCA_RS02960 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00260.hmm # target sequence database: /tmp/gapView.928.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00260 [M=340] Accession: TIGR00260 Description: thrC: threonine synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.4e-96 307.0 0.0 1.2e-95 306.5 0.0 1.2 1 lcl|NCBI__GCF_000008325.1:WP_010959941.1 MCA_RS02960 threonine synthase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000008325.1:WP_010959941.1 MCA_RS02960 threonine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 306.5 0.0 1.2e-95 1.2e-95 1 340 [] 11 352 .. 11 352 .. 0.98 Alignments for each domain: == domain 1 score: 306.5 bits; conditional E-value: 1.2e-95 TIGR00260 1 vvryreilevt.ekdlvdlaegstelfrspklaeevga.enlyvkelfhgPtlaFKDrglqfvavlltk 67 ++ryr l+v+ e+++++l eg t+l+r +++++ +g +lyvk ++++Pt++FKDrg +++++t+ lcl|NCBI__GCF_000008325.1:WP_010959941.1 11 IERYRDRLPVSaETRIISLGEGDTPLIRLENIPRLLGRrVELYVKYEGLNPTGSFKDRG---MTMAVTR 76 78************************************99*******************...******* PP TIGR00260 68 alelgnetvlcAtsGdtgaaaaealagkanvkvvvLyPkgkispvkeklvtalaenakvlaikGdFDda 136 a e+g +++cA++G+t+aaaa a+a++a++ ++vL+P+gki+ +kl++a++++ v++ikG+FDd lcl|NCBI__GCF_000008325.1:WP_010959941.1 77 AVEEGSRAIICASTGNTSAAAA-AYAARAGITAFVLIPDGKIA--MGKLAQAMMYGSVVIQIKGNFDDG 142 **********************.********************..************************ PP TIGR00260 137 qdlvkeifedkeklklnsvNsinparieaqktyafeiveqlgkespdkvvvpvp.sgnfgailkGflek 204 +lvke++++ + + +ns+N p+r+++qkt+afeiv++lg ++pd ++pv+ +gn++a + G+ e+ lcl|NCBI__GCF_000008325.1:WP_010959941.1 143 MRLVKEVAGEAPVTIVNSIN---PYRLQGQKTAAFEIVDELG-RAPDYHCLPVGnAGNITAHWIGYSEY 207 ********998877777776...*******************.9************************* PP TIGR00260 205 kelg..........................lpieklaiaaegaadivrrflksgdlepkedkeTlstAm 247 +g +p ++ + +a g+a+++r ++eT++tA+ lcl|NCBI__GCF_000008325.1:WP_010959941.1 208 ACGGrdcvteacafcngecrfvsggpvstsRP-KMVGYQASGSAPFLRGH-------MVDHPETVATAI 268 *****************************999.**********9999988.......67789******* PP TIGR00260 248 dignpsnveralelarrslgnledlkesvsdeeileaikklaeeegyllephtavavaalkklvekgvs 316 +ig+p ++e+a+++ r+s+g +++ sdeeil a+k+l e+eg+++ep++a+++a+ ++ + +g lcl|NCBI__GCF_000008325.1:WP_010959941.1 269 RIGHPQSWEQAWQVSRESGGWFDEC----SDEEILAAQKLLTEREGVFCEPASATSLAGALRDIRSG-- 331 ************************9....**************************************.. PP TIGR00260 317 atadpaKFeevvealtgnklkdpe 340 +++++ ++vv+ ltgn+lkdp+ lcl|NCBI__GCF_000008325.1:WP_010959941.1 332 -KIPEG--STVVLTLTGNGLKDPD 352 .*****..**************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (340 nodes) Target sequences: 1 (380 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 12.91 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory