Alignments for a candidate for ilvI in Methanothermobacter thermautotrophicus Delta H

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Methanothermobacter thermautotrophicus Delta H

Align Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate WP_010877210.1 MTH_RS07675 thiamine pyrophosphate-binding protein

Query= curated2:O08353
         (599 letters)



>NCBI__GCF_000008645.1:WP_010877210.1
          Length = 524

 Score =  332 bits (851), Expect = 2e-95
 Identities = 203/549 (36%), Positives = 297/549 (54%), Gaps = 40/549 (7%)

Query: 4   AEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARASGK 63
           AE +   LE      +FG+PG  +LP Y+AL  S L H+L RHEQ A HAADGYARASG 
Sbjct: 5   AEVLAGILEEAGFTHIFGHPGEQILPLYEALRKSGLEHVLMRHEQGAVHAADGYARASGG 64

Query: 64  VGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLFMPIVK 123
            GVC+ T GPGA NLV GVA A +DS P++A+TG +P +  G   FQE+D   +F  + +
Sbjct: 65  PGVCVATGGPGALNLVMGVAAASTDSVPLIAMTGDLP-RGGGPGRFQEVDLEAVFRALTR 123

Query: 124 HNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDIDKHPIPSKVKLIGYN 183
            +       +   +   +    + G  G +HI+LP+DV  LE D      PS+V      
Sbjct: 124 MSMTPGSGDEASMMLADSISSLRRGLTGVIHINLPRDV--LEEDACPPVGPSEV------ 175

Query: 184 PTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTLMGKGC 243
                    + +A++L+ SA+RP+ILAGGGV+   A  E  + +E   IPV TT   +G 
Sbjct: 176 ---FRELPDMSRALELLGSARRPLILAGGGVIWGNAVGEFREFIENARIPVVTTYSARGV 232

Query: 244 ISENHPLALGMVGMHGTKPANYCLSESDVLISIGCRFSDRITGDIKSFATNAKIIHIDID 303
           I E+HPL LGM G  GT  AN+     DVL+ +G R S+R      +   N ++IH++ D
Sbjct: 233 IPEDHPLCLGMTGTRGTPAANHAAGNCDVLLVLGARLSERTL----AATGNPRVIHVNTD 288

Query: 304 PAEIGKNVNVDVPIVGDAKLILKEVIKQLDYIINKDSKENNDKENISQWIENVNSLKKSS 363
           P+ +  +V +++  VGD    L E+                  +    W + + + ++ +
Sbjct: 289 PSVLRGDVVLEMD-VGDFLRELPEL------------------KGPEGWFKELAAYRRDN 329

Query: 364 IPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQNQMWMAHYFKTQTPRSFLS 423
            P+ +    P      V    AV   L+   +  I  D G +  W+  + +    RS + 
Sbjct: 330 PPISELQ--PPGGGAFVTS-SAVRGILDAAPDATIVNDAGSHTTWVTLHRRVLRERSLIF 386

Query: 424 SGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNIPVVICIFDNR 483
           SGG G MG+G P+AIGAK+A P   VI I GDGGF M  QELGT+AE  +P+ +CI +N+
Sbjct: 387 SGGFGPMGYGLPAAIGAKLADPSRDVIMIAGDGGFQMTIQELGTVAELELPITMCILNNQ 446

Query: 484 TLGMVYQWQNLFYGKRQCSVNFGGAPDFIKLAESYGIKARRIESPNEINEALKEAINCDE 543
            L ++ QWQ + YG    SV     PDFI+LA++YGI A RI    +++ A++ A+   E
Sbjct: 447 KLDVIRQWQEMNYG-TSYSVELKN-PDFIRLADAYGIGAERILRLEDVSGAVERALESGE 504

Query: 544 PYLLDFAID 552
           P LL+F ++
Sbjct: 505 PRLLEFMVE 513


Lambda     K      H
   0.319    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 813
Number of extensions: 37
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 599
Length of database: 524
Length adjustment: 36
Effective length of query: 563
Effective length of database: 488
Effective search space:   274744
Effective search space used:   274744
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis