Alignments for a candidate for ilvC in Clostridium acetobutylicum ATCC 824

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvC in Clostridium acetobutylicum ATCC 824

Align Ketol-acid reductoisomerase (NAD(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.382 (characterized)
to candidate NP_346736.1 CA_C0091 ketol-acid reductoisomerase

Query= SwissProt::K4LVZ1
         (332 letters)



>NCBI__GCF_000008765.1:NP_346736.1
          Length = 337

 Score =  424 bits (1091), Expect = e-123
 Identities = 202/329 (61%), Positives = 248/329 (75%)

Query: 1   MKIYYDQDADLQYLDGKTVAVIGYGSQGHAQSQNLRDSGVKVVVADIPSSENWKKAEEAQ 60
           +K+YYD+DADL YL  K +A+IG+GSQGHA + NL++SG+ V+V     S++WK AE+  
Sbjct: 4   LKVYYDEDADLNYLKDKKIAIIGFGSQGHAHALNLKESGLNVIVGLYDGSKSWKVAEDYG 63

Query: 61  FQPLTADEAAREADIIQILVPDEKQAALYRESIAPNLRPGKALVFSHGFNIHFKQIVPPP 120
           F+     +A ++A ++ IL+PDEKQ  +Y  SI  NL  G AL F+HGFNIH+ QIVPP 
Sbjct: 64  FEVYEVADAVKQAQVVMILLPDEKQKQIYEASIKDNLDEGDALFFAHGFNIHYNQIVPPK 123

Query: 121 DVDVFMVAPKGPGHLVRRMYEEGAGVPSLVAVEQDYSGQALNLALAYAKGIGATRAGVIQ 180
           +VDV M+APKGPGH+VRR Y EG GVP L AV QDY+G+   +ALAY KGIG T+ GV+ 
Sbjct: 124 NVDVLMIAPKGPGHIVRRQYTEGGGVPCLYAVHQDYTGKGKEIALAYGKGIGGTKGGVMN 183

Query: 181 TTFKEETETDLFGEQAVLCGGITELIRAGFDTLVDAGYQPEIAYFECLHEMKLIVDLIYE 240
           TTFK ETETDLFGEQAVLCGGI  LI AG+DTL +AGY  E AYFEC HEMK+IVDL+YE
Sbjct: 184 TTFKIETETDLFGEQAVLCGGICALINAGYDTLREAGYSAENAYFECFHEMKMIVDLMYE 243

Query: 241 GGISTMRYSISDTAEYGDLTRGKRIITEATREEMKKILKEIQDGVFAREWLLENQVGRPV 300
           GG++ MRYSISDTAEYGD   G R+I +  R EMKK+L EIQDG FA+ WLLENQ GRP 
Sbjct: 244 GGMAKMRYSISDTAEYGDYVVGNRLINDNVRAEMKKVLTEIQDGTFAKNWLLENQTGRPA 303

Query: 301 YNALRRKEQNHLIETVGARLRGMMPWLKK 329
           +NA RR E +  IE VG RLRGMM W+ +
Sbjct: 304 FNARRRIEADREIEKVGKRLRGMMSWINE 332


Lambda     K      H
   0.318    0.136    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 377
Number of extensions: 10
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 332
Length of database: 337
Length adjustment: 28
Effective length of query: 304
Effective length of database: 309
Effective search space:    93936
Effective search space used:    93936
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate NP_346736.1 CA_C0091 (ketol-acid reductoisomerase)
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00465.hmm
# target sequence database:        /tmp/gapView.23797.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00465  [M=314]
Accession:   TIGR00465
Description: ilvC: ketol-acid reductoisomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                              Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                              -----------
   2.7e-142  459.3   1.1   3.1e-142  459.1   1.1    1.0  1  lcl|NCBI__GCF_000008765.1:NP_346736.1  CA_C0091 ketol-acid reductoisome


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000008765.1:NP_346736.1  CA_C0091 ketol-acid reductoisomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  459.1   1.1  3.1e-142  3.1e-142       1     312 [.      17     329 ..      17     331 .. 0.99

  Alignments for each domain:
  == domain 1  score: 459.1 bits;  conditional E-value: 3.1e-142
                              TIGR00465   1 lkgkkvaiiGyGsqGeaqalnlrdsglnvivglrkeaaswkkAeedGfkvltveeaikkadlimiLlpDevq 72 
                                            lk+kk+aiiG+GsqG+a+alnl++sglnvivgl+ +++swk Ae+ Gf+v +v++a+k+a+++miLlpDe q
  lcl|NCBI__GCF_000008765.1:NP_346736.1  17 LKDKKIAIIGFGSQGHAHALNLKESGLNVIVGLYDGSKSWKVAEDYGFEVYEVADAVKQAQVVMILLPDEKQ 88 
                                            79********************************************************************** PP

                              TIGR00465  73 kevyeaeikpllkegkallfsHGfnivfkqivipkdvdvvlvAPKgpGalvReeykegrGvpsliAveqdvt 144
                                            k+ yea+ik++l eg+al f+HGfni+++qiv+pk+vdv+++APKgpG+ vR++y eg Gvp l+Av+qd+t
  lcl|NCBI__GCF_000008765.1:NP_346736.1  89 KQIYEASIKDNLDEGDALFFAHGFNIHYNQIVPPKNVDVLMIAPKGPGHIVRRQYTEGGGVPCLYAVHQDYT 160
                                            ************************************************************************ PP

                              TIGR00465 145 geakeiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLveaGyqpelAyfeivh 216
                                            g++keiAlay k+iGg++ gv++ttFk E+e+DLfGEqavLcGg+ ali+a++dtL+eaGy+ e Ayfe+ h
  lcl|NCBI__GCF_000008765.1:NP_346736.1 161 GKGKEIALAYGKGIGGTKGGVMNTTFKIETETDLFGEQAVLCGGICALINAGYDTLREAGYSAENAYFECFH 232
                                            ************************************************************************ PP

                              TIGR00465 217 elklivdllkekGlelmrdavsntAklgalelr.eilkeelkkemqkilkeiqnGefakewalekeagkpaf 287
                                            e+k+ivdl++e+G+++mr ++s+tA++g++ ++ + +++++++em+k+l eiq+G+fak+w+le+++g+paf
  lcl|NCBI__GCF_000008765.1:NP_346736.1 233 EMKMIVDLMYEGGMAKMRYSISDTAEYGDYVVGnRLINDNVRAEMKKVLTEIQDGTFAKNWLLENQTGRPAF 304
                                            ********************************99************************************** PP

                              TIGR00465 288 eearkkekeqeiekvGkelralvka 312
                                            ++ r+ e ++eiekvGk+lr ++++
  lcl|NCBI__GCF_000008765.1:NP_346736.1 305 NARRRIEADREIEKVGKRLRGMMSW 329
                                            **********************987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (314 nodes)
Target sequences:                          1  (337 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 7.63
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis