Alignments for a candidate for ilvI in Rhizobium leguminosarum 3841

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Rhizobium leguminosarum 3841

Align Acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate WP_011649047.1 RL_RS34280 acetolactate synthase large subunit

Query= curated2:Q7U5G1
         (617 letters)



>NCBI__GCF_000009265.1:WP_011649047.1
          Length = 548

 Score =  238 bits (606), Expect = 7e-67
 Identities = 170/547 (31%), Positives = 267/547 (48%), Gaps = 24/547 (4%)

Query: 23  GAAALMDALRRHGVDTIFGYPGGAILPIYDALHIAESEGWVKHILVRHEQAGTHAADAYA 82
           G+  L+ A+   GVD IFG PG   L + +++  +     ++ +L RHEQA    A  Y 
Sbjct: 4   GSDLLVAAIENEGVDRIFGIPGEENLDVVESIRKSS----IELVLTRHEQAAAFMAATYG 59

Query: 83  RATGKVGVCFGTSGPGATNLVTGIATAQMDSVPMVVITGQVPRPAIGTDAFQETDIFGIT 142
           R TGK GVC  T GPGA NL TG A A + ++PMV+ITGQ    +     FQ  D+    
Sbjct: 60  RLTGKPGVCLTTLGPGALNLSTGAAYALLGAMPMVMITGQKGILSSRQARFQVVDVVASM 119

Query: 143 LPIVKHSWVVRDPADLGSIVAQAFLIAASGRPGPVLIDIPKDVGQEQFNYVPVEPGSVIP 202
            P+ K +  +  P  + + V +AF IA   +PGPV +++P+D+  E+   V +    + P
Sbjct: 120 KPLTKLARQIVSPQMIPTTVREAFRIAQEEKPGPVHLELPEDIAAEECQEVAL----IAP 175

Query: 203 GGFHQPEPPL--DAAVAAALDLIEQAQRPLLYVGGGAISACAHDSLRMLAERYQLPVTTT 260
              HQ E P   DAA+  A DLI  A+RPLL  G  A    +   +     R ++P  TT
Sbjct: 176 ---HQLELPTASDAALDRAADLIAAAKRPLLMFGAAASRPRSTSDIAQFVIRTRIPFFTT 232

Query: 261 LMGKGAFDENDALSVGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDTFAPRARV 320
            MGKG       L +G   +    Y + A+ + DL+I +G    ++    +    P  +V
Sbjct: 233 QMGKGTVPGGTELYMGTAALSERDYVHEAIEQADLIITIGHDTIEKPPFIMGKGGP--KV 290

Query: 321 VHFEIDPAEIGKNRKADVAVLGDLGLSLARMVEISLQRTAEPRTAAWLERINTWKDRYPL 380
           VH    PA + +       V+GD+G SL  + +    +   P   A L       +R  +
Sbjct: 291 VHVGYQPATVEQVYFPQSEVIGDIGPSLKALADRLEGKL--PNAHALLHLRERILER--I 346

Query: 381 TIPPAEGAIYPQEVLLAVRDLAP-DAIVTTDVGQHQMWAAQHLRNG-PRGWISSAGLGTM 438
                E    PQ ++  +R++ P D I+  D G +++W A++ R       +    L TM
Sbjct: 347 AARATEDRFTPQRLVHDIREVMPHDGILALDNGMYKIWFARNYRTRMANTLLLDNALATM 406

Query: 439 GFGMPAAMGAQVAMPDRQVVCIAGDASILMNIQELGTLAAYGLPVKVVIVNNHWQGMVRQ 498
           G G+P+AM A +  P+R+V+ I GD   +MN QEL T     L + V+++ ++  GM+R 
Sbjct: 407 GAGLPSAMVASMLYPERRVMAICGDGGFMMNSQELETAVRLKLNLVVLVIEDNAYGMIR- 465

Query: 499 WQESFYDERYSASDMLNGMPDFIALARSFGVDGVKITDRELLHRDLAAALQSPTPTMIDV 558
           W+++   + +    M  G PDF+  A S+G  G ++ D     + L  A       +++V
Sbjct: 466 WKQAV--DEFPDFGMTFGNPDFVKYAESYGARGTRVEDISQFKQVLEEAFSGGGVHLVNV 523

Query: 559 HVRRGEN 565
            V   EN
Sbjct: 524 PVDYSEN 530


Lambda     K      H
   0.320    0.136    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 766
Number of extensions: 38
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 617
Length of database: 548
Length adjustment: 36
Effective length of query: 581
Effective length of database: 512
Effective search space:   297472
Effective search space used:   297472
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis