Alignments for a candidate for hom in Azorhizobium caulinodans ORS 571

GapMind for Amino acid biosynthesis

Alignments for a candidate for hom in Azorhizobium caulinodans ORS 571

Align homoserine dehydrogenase (EC 1.1.1.3); aspartate kinase (EC 2.7.2.4) (characterized)
to candidate WP_012173009.1 AZC_RS23020 aspartate kinase

Query= BRENDA::Q9WZ17
         (739 letters)



>NCBI__GCF_000010525.1:WP_012173009.1
          Length = 420

 Score =  267 bits (682), Expect = 9e-76
 Identities = 153/417 (36%), Positives = 249/417 (59%), Gaps = 19/417 (4%)

Query: 341 VVMKFGGAAISDVEKLEKVAEKIIKRKKSGVKPVVVLSAMGDTTDHLIELAKTIDENPDP 400
           +VMKFGG +++++E++  VA  + +   +G +  VV+SAM   T+ L+   K      DP
Sbjct: 4   LVMKFGGTSVANIERIRNVARHVKREVDAGYEVAVVVSAMSGKTNELVAWCKESSALYDP 63

Query: 401 RELDLLLSTGEIQSVALMSIALRKRGYKAISFTGNQLKIITDKRYGSARIIDINTDIISR 460
           RE D ++++GE  +  L++I L+  G KA S+ G Q+ I TD+ +GSARI +I+   ++ 
Sbjct: 64  REYDAVVASGEQVTSGLLAITLQSMGLKARSWQGWQIPISTDEAHGSARIQEIDGTALNT 123

Query: 461 YLKQDFIPVVAGFQGI-TETGDITTLGRGGSDLTAIALAYSLGADLCELYKDVDGVYTAD 519
                 + V+AGFQG+   TG ITTLGRGGSD +A+A+A ++GA+ C++Y DVDGVYT D
Sbjct: 124 GFAAGEVAVIAGFQGMHLPTGRITTLGRGGSDTSAVAVAAAIGAERCDIYTDVDGVYTTD 183

Query: 520 PRIVKDARVIKELSWEEMIELSRHGAQVLQARAAEFARKYGVKVLIKNAHK--------- 570
           PR+V  A+ +  +++EEM+E++  GA+VLQ R+ E A  + V+  ++++ +         
Sbjct: 184 PRVVPKAKRLDRIAFEEMLEMASLGAKVLQVRSVELAMVHQVRTFVRSSFEDPDAPHMGT 243

Query: 571 -ETRGTLIWE-----GTKVENPIVRAVTFEDGMAKVVLKDVPDKPGVAARIMRTLSQMGV 624
            E+ GTLI +       ++E  +V  + F    A+V ++ V DKPGVAA +   L+   +
Sbjct: 244 VESAGTLICDEEEIVANQMEARVVTGIAFSKDEAQVSIRRVEDKPGVAAAVFVPLADAHI 303

Query: 625 NIDMIIQGMKS-GEYNTVAFIVPESQLGKLDIDLLKTRSEAKEIIIE--KGLAKVSIVGV 681
           N+DMI+Q + + G    + F VP +   +    L K R       IE    + KVS++G+
Sbjct: 304 NVDMIVQNVSADGRTTDITFTVPTADYERAKDVLNKARDTITFETIEGATDVTKVSVIGI 363

Query: 682 NLTSTPEISATLFETLANEGINIDMISASSSRISVIIDGKYVEDAVKAIHSRFELDR 738
            + S   ++A  F+ LA +GINI  I+ S  +IS++ID  Y E AV+ +HS + LD+
Sbjct: 364 GMRSHAGVAADAFKALAAKGINIRAITTSEIKISILIDAAYTELAVRTLHSLYGLDK 420



 Score = 26.6 bits (57), Expect = 0.003
 Identities = 54/294 (18%), Positives = 111/294 (37%), Gaps = 49/294 (16%)

Query: 212 DVAYKVSVLAGVVTGRFPGINSVQFEGITRIDPEYLKEIVRSGKK---------LKLIG- 261
           D  Y+V+V+   ++G+   + +   E     DP     +V SG++         L+ +G 
Sbjct: 31  DAGYEVAVVVSAMSGKTNELVAWCKESSALYDPREYDAVVASGEQVTSGLLAITLQSMGL 90

Query: 262 --------ELDFSTNRYEVRLREVTPEDPFFNVDGVDNAIEVSTDLAGDFLLKGR----G 309
                   ++  ST+      R    +    N       + V     G  L  GR    G
Sbjct: 91  KARSWQGWQIPISTDEAHGSARIQEIDGTALNTGFAAGEVAVIAGFQGMHLPTGRITTLG 150

Query: 310 AGGYPTASAVIADLFRVAKYKVLGGAEKFSVV-----VMKFGGAAISDVEKLEKVA-EKI 363
            GG  T++  +A            GAE+  +      V       +   ++L+++A E++
Sbjct: 151 RGGSDTSAVAVAAAI---------GAERCDIYTDVDGVYTTDPRVVPKAKRLDRIAFEEM 201

Query: 364 IKRKKSGVKPVVVLSAMGDTTDHLIELAKTIDENPDPRELDLLLSTGEI--QSVALMSIA 421
           ++    G K + V S        +    ++  E+PD   +  + S G +      +++  
Sbjct: 202 LEMASLGAKVLQVRSVELAMVHQVRTFVRSSFEDPDAPHMGTVESAGTLICDEEEIVANQ 261

Query: 422 LRKRGYKAISFTGNQLKI----ITDKRYGSARII------DINTDIISRYLKQD 465
           +  R    I+F+ ++ ++    + DK   +A +        IN D+I + +  D
Sbjct: 262 MEARVVTGIAFSKDEAQVSIRRVEDKPGVAAAVFVPLADAHINVDMIVQNVSAD 315


Lambda     K      H
   0.318    0.137    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 658
Number of extensions: 28
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 739
Length of database: 420
Length adjustment: 36
Effective length of query: 703
Effective length of database: 384
Effective search space:   269952
Effective search space used:   269952
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis