Alignments for a candidate for ilvD in Azorhizobium caulinodans ORS 571

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Azorhizobium caulinodans ORS 571

Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized)
to candidate WP_043880120.1 AZC_RS07250 dihydroxy-acid dehydratase

Query= curated2:Q8TW40
         (549 letters)



>NCBI__GCF_000010525.1:WP_043880120.1
          Length = 571

 Score =  357 bits (917), Expect = e-103
 Identities = 217/539 (40%), Positives = 299/539 (55%), Gaps = 15/539 (2%)

Query: 16  RALLRACGLTDEEMDRPFVAVVNTYSEVVPGHMHLDKVTEAVKAGIRMAGGVPFEVETIA 75
           +A ++A G +D+ +DRP + + NT+S+  P H ++ ++ EAVK G+ +AGG+P E  T++
Sbjct: 33  KAFIKAAGYSDDALDRPIIGITNTFSDFNPCHGNVPRLIEAVKRGVMLAGGLPMEFPTVS 92

Query: 76  LCDGIAMNTPGMKYSLPSRELVADTIETVIEAHRFDGFVAIVSCDKMVPGALMAAARLDL 135
           + +  +  T     S+  R L+A   E +I A   D  V I  CDK VP  LM A   ++
Sbjct: 93  IHESFSNPT-----SMFLRNLMAMDTEEMIRAQPMDAVVLIGGCDKTVPAQLMGAVSANV 147

Query: 136 PAAIVTGGPMEPGCVDGERVDLI-DAFEAVGAYEEGEISEEELEELEQRACPGPGSCAGM 194
           PA  +  GPM  G   GE +    D     G +  G +  EE++ L  R  P  G+C  M
Sbjct: 148 PAIQLITGPMLVGHHKGEILGACTDCRRLWGQHRAGHLDAEEMDVLSGRLAPTQGTCMVM 207

Query: 195 FTANTMACMTEVLGMSEFNCAATPATEAEKLRVAKLTGMRIVEAIEEGITARDVLTREAF 254
            TA+TM C+ E LG++       PAT A+++R A+ +G + V+    G T RD+LT  AF
Sbjct: 208 GTASTMGCLVEALGIALPWTGTIPATHADRIRAAEASGRQAVQLATHGPTPRDLLTPAAF 267

Query: 255 LDAIRVDMALGGSTNTVLHLLAIAREADVELSLDDFDELSRETPHLCAMRPGGPYTMRDL 314
            +A+ V  A+GGSTN ++HL AIAR A + + LD FD + R  P L  ++P G + M   
Sbjct: 268 RNALTVLQAIGGSTNGLVHLAAIARRAGLSVDLDAFDAIGRSVPVLIDLKPSGDHYMEHF 327

Query: 315 YEAGGVPAVMKELADDLHLDRIDFAGRSMRERVERT-EVKDREVIRPKEDPVHEEGGIVV 373
           + AGGVP +MKEL D L        GR++ E  +   EV  + +IRP   P+   GG+ V
Sbjct: 328 HWAGGVPKLMKELGDHLDRSAPTIDGRTIGEIADAAEEVPGQAIIRPASAPLKPFGGMAV 387

Query: 374 LYGNLAPKGAVIKTAALSEEMYEHEGPAVVFDSEEEATEAI--LGGDIDPGDVVVIRYEG 431
           L GNLAP GAVIK +A SE +  H G AVVFDS E+    I   G D+   DV+V+R  G
Sbjct: 388 LRGNLAPGGAVIKHSAASEALLRHTGRAVVFDSVEDMVNRIDDPGLDVQADDVLVLRNAG 447

Query: 432 PAGGPGMRE--MLTPTAALCGMGLDDSVALVTDGRFSGGTRGPCVGHVSPEAYRGGPIAV 489
           P G PGM E   L     L   G+ D V  ++D R SG   G  V H++PEA  GGP+ +
Sbjct: 448 PKGAPGMPEAGYLPIPKKLAQAGVKDMVR-ISDARMSGTAFGTIVLHITPEAAVGGPLGL 506

Query: 490 VEEGDTIRLDVRERRLEVDVEDEELEARLEEWEPP---EDEVTGYLRRYRELVRGADEG 545
           V  GDTIRLDV  R + + V DEELE R  EW PP    +   GY   Y   V  ADEG
Sbjct: 507 VRSGDTIRLDVAAREIALLVPDEELERRRAEWTPPAHLAEAARGYQHLYLTHVLQADEG 565


Lambda     K      H
   0.317    0.137    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 902
Number of extensions: 48
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 549
Length of database: 571
Length adjustment: 36
Effective length of query: 513
Effective length of database: 535
Effective search space:   274455
Effective search space used:   274455
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis