Alignments for a candidate for thrB in Azospirillum lipoferum B510

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrB in Azospirillum lipoferum B510

Align homoserine kinase (EC 2.7.1.39) (characterized)
to candidate WP_012974150.1 AZL_RS08145 homoserine kinase

Query= reanno::Korea:Ga0059261_3253
         (319 letters)



>NCBI__GCF_000010725.1:WP_012974150.1
          Length = 327

 Score =  281 bits (720), Expect = 1e-80
 Identities = 142/310 (45%), Positives = 196/310 (63%), Gaps = 2/310 (0%)

Query: 1   MAVYTQVSAEALAGFLARYDVGELVSAKGIAEGVENSNYLVETTRDRFILTLYEKRVEAA 60
           MAVYT+V+ E L+ F A YD+G ++S KGIAEGVENSN+L+ T R  +ILTLYEKR    
Sbjct: 1   MAVYTEVTDEDLSSFAAEYDLGAVLSCKGIAEGVENSNFLLVTERGPYILTLYEKRTRRE 60

Query: 61  DLPYFMGLLDHLAAKGLPVPPAIKDRGGVEIQELNGRPACLIKFLSGISLSHPTPAQARA 120
           DLP+F+GL++HLA KG+  P  +  R GV ++EL GRPA ++ FL+G+     TP     
Sbjct: 61  DLPFFLGLMEHLADKGIACPLPVAARDGVALRELCGRPAVIVTFLAGMWPRRITPQHCAQ 120

Query: 121 AGEAMAQMHRAVADFPLDRPNSMGVDTWQPLFEKCGHSLDQIVPGLYDDLGFAIARVVPA 180
            GEA+A++H AV DF L+RPN++ +  W+ LF +     D++ PGL   L   +A +   
Sbjct: 121 LGEALARLHLAVGDFRLERPNALALPGWKELFARSAERADEVAPGLRATLEAELAALEVN 180

Query: 181 WTRNDFDRCAIHADLFPDNVLMRGDQVTGLIDFYFACTDIRVYDLAVMHSAWSFDAHGRN 240
           W         IHADLFPDNV  RGD ++GLIDFYFAC D   YD+A+  +AW F+  G +
Sbjct: 181 WPAG-LPAGVIHADLFPDNVFFRGDSLSGLIDFYFACNDFLAYDVAICVNAWCFEIDG-S 238

Query: 241 YDPAVGDALIAGYEASFPLTEVERAAFPTLAAGACIRFSLSRAWDWLNTPADALVMRKDP 300
           ++      L++ Y    PL+  E  A P L  G+ +RF L+R +DWLN P  A V  KDP
Sbjct: 239 FNATKARLLLSNYRKVRPLSRAELDALPWLCRGSAVRFLLTRLYDWLNHPPGAFVRPKDP 298

Query: 301 LAYVRRLKHY 310
           L Y+R+L+ +
Sbjct: 299 LEYLRKLRFH 308


Lambda     K      H
   0.323    0.138    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 305
Number of extensions: 9
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 319
Length of database: 327
Length adjustment: 28
Effective length of query: 291
Effective length of database: 299
Effective search space:    87009
Effective search space used:    87009
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 48 (23.1 bits)

Align candidate WP_012974150.1 AZL_RS08145 (homoserine kinase)
to HMM TIGR00938 (thrB: homoserine kinase (EC 2.7.1.39))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00938.hmm
# target sequence database:        /tmp/gapView.16259.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00938  [M=307]
Accession:   TIGR00938
Description: thrB_alt: homoserine kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   7.6e-123  395.7   0.0   8.7e-123  395.5   0.0    1.0  1  lcl|NCBI__GCF_000010725.1:WP_012974150.1  AZL_RS08145 homoserine kinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000010725.1:WP_012974150.1  AZL_RS08145 homoserine kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  395.5   0.0  8.7e-123  8.7e-123       1     307 []       1     307 [.       1     307 [. 1.00

  Alignments for each domain:
  == domain 1  score: 395.5 bits;  conditional E-value: 8.7e-123
                                 TIGR00938   1 mavytsvsdeeleafLegydlGellslkGiaeGvensnyllttdkgryvLtlyekrvkaeeLPfflell 69 
                                               mavyt+v+de+l++f   ydlG++ls kGiaeGvensn+ll t++g y+Ltlyekr++ e+LPffl l+
  lcl|NCBI__GCF_000010725.1:WP_012974150.1   1 MAVYTEVTDEDLSSFAAEYDLGAVLSCKGIAEGVENSNFLLVTERGPYILTLYEKRTRREDLPFFLGLM 69 
                                               9******************************************************************** PP

                                 TIGR00938  70 thLaerglpvakpvksrdGralseLaGkPaalvefLkGssvakPtaercrevgevlaklhlagadfkee 138
                                               +hLa++g++++ pv+ rdG al eL G+Pa++v fL+G+  ++ t+++c++ ge la+lhla  df+ e
  lcl|NCBI__GCF_000010725.1:WP_012974150.1  70 EHLADKGIACPLPVAARDGVALRELCGRPAVIVTFLAGMWPRRITPQHCAQLGEALARLHLAVGDFRLE 138
                                               ********************************************************************* PP

                                 TIGR00938 139 rkndlrleaWsilaakkfkvleqleeelaalldkeldalkkflprdLPrgvihadlfkdnvlldgdklk 207
                                               r n l l  W+ l+a+  + ++++ ++l a+l++el+al+ ++p  LP gvihadlf+dnv++ gd+l+
  lcl|NCBI__GCF_000010725.1:WP_012974150.1 139 RPNALALPGWKELFARSAERADEVAPGLRATLEAELAALEVNWPAGLPAGVIHADLFPDNVFFRGDSLS 207
                                               ********************************************************************* PP

                                 TIGR00938 208 gvidfyfaCedallydlaiavndWcfeaddkldaaaakallkgyeavrpLseeekaafpvllrgaalrf 276
                                               g+idfyfaC+d l+yd+ai+vn+Wcfe d++++a++a+ ll  y++vrpLs  e +a+p l rg+a+rf
  lcl|NCBI__GCF_000010725.1:WP_012974150.1 208 GLIDFYFACNDFLAYDVAICVNAWCFEIDGSFNATKARLLLSNYRKVRPLSRAELDALPWLCRGSAVRF 276
                                               ********************************************************************* PP

                                 TIGR00938 277 llsrlldlvftqagelvvakdPaeferkLkv 307
                                               ll+rl d+ + ++g++v++kdP e+ rkL++
  lcl|NCBI__GCF_000010725.1:WP_012974150.1 277 LLTRLYDWLNHPPGAFVRPKDPLEYLRKLRF 307
                                               ****************************985 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (307 nodes)
Target sequences:                          1  (327 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 8.72
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis