Alignments for a candidate for ilvD in Azospirillum lipoferum B510

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Azospirillum lipoferum B510

Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized)
to candidate WP_012976399.1 AZL_RS20625 phosphogluconate dehydratase

Query= curated2:B8DMM1
         (555 letters)



>NCBI__GCF_000010725.1:WP_012976399.1
          Length = 612

 Score =  261 bits (666), Expect = 7e-74
 Identities = 176/514 (34%), Positives = 270/514 (52%), Gaps = 34/514 (6%)

Query: 31  RPLVGVVNAANEVVPGHVHLHTIAEAVKAGVRAAGGTPMEFPAI-AVCDGLAMNHEGMHF 89
           +P +G+V A N+++  H         ++  VRAAGG       + A+CDG+   +EGM  
Sbjct: 70  QPSIGIVTAYNDMLSAHQPYEHYPALIRQSVRAAGGVAQVAGGVPAMCDGVTQGYEGMEL 129

Query: 90  SLPSREIIADSIEIMATAHPFDALVFIPNCDKSVPGMLMAMLRMD-IPSIMVSGGPMLAG 148
           SL SR++IA +  +  +   FD ++ +  CDK VPG+++  L    +P+I V  GPM +G
Sbjct: 130 SLFSRDVIALATGVALSHATFDGVLCLGICDKIVPGLVIGSLAFGHLPTIFVPAGPMPSG 189

Query: 149 GTLAGRTDLISVFEAVGRVQRGDMTMAELDEMTETACPGCGSCAGMFTANTMNCMAETMG 208
            + A +  +  ++ A G++ R ++  AE       A    G+C    TANT   + E MG
Sbjct: 190 LSNADKAKVRQLY-AQGKIGRDELLEAE-----SQAYHAPGTCTFYGTANTNQMLMEIMG 243

Query: 209 LALPGNGTIPAVTAARVRLAKHAGMKVMELLEKNITPR--SIVTPRAVANAVAVDMALGG 266
           L LPG   +   T  R  L   A  +V+ +           I   RA+ N +   +A GG
Sbjct: 244 LHLPGASFVNPDTPLRDALTDAAARRVVAMAAPGAGSPIGRIADERALVNGIVGLLATGG 303

Query: 267 STNTVLHLPAVFGEAGLDLTLDIFDEVSRKTPNLCKLSPAGHHHIQDLHAAGGIPAVMAE 326
           STN  LH+PA+   AG++LT + F ++S   P L ++ P G   +   H AGG+P ++ +
Sbjct: 304 STNHTLHIPAIAAAAGIELTWEDFSDLSAVVPLLARVYPNGSADVNRFHQAGGMPFIIGQ 363

Query: 327 LTRKGLVDTSVMTVTGKTLAE---NLAELNA------------RVLNPDVIRSADAPYSA 371
           L   GLV T + TV G+   E   ++ EL+             +  +P V+ +  AP++A
Sbjct: 364 LIDAGLVHTDIATVWGEGGLEPYRSMPELDEGGALSWTRSATDQSGDPTVVATTAAPFAA 423

Query: 372 QGGIAILKGSLAPQGAVVKQSAVAPEMMVREAVARVFDSEGEAHAAIMGGKINKGDAIII 431
           +GG+ +L G+L     V+K SAV PE  V EA ARVFD++    AA   G +++   +++
Sbjct: 424 EGGLRVLTGNLG--RGVIKISAVKPEHRVIEAPARVFDNQEAVQAAFRAGDLDRDVVVVV 481

Query: 432 RYEGP--RGGPGMREMLSPTAAIAGMGLGADVALITDGRFSG--GTRGAAIGHVSPEAAD 487
           RY+GP   G P + ++  P   +   G    VAL+TDGR SG  G   AAI HV+PE   
Sbjct: 482 RYQGPAANGMPELHKLTPPLGVLQDKGF--KVALVTDGRMSGASGKVPAAI-HVTPEVKG 538

Query: 488 GGNIGLVREGDHILIDIPARRLDLLVDEAELAAR 521
           GG +G VR+GD + +D  A  LD LVD AE  +R
Sbjct: 539 GGPLGRVRDGDILRLDAEAGTLDALVDSAEWQSR 572


Lambda     K      H
   0.319    0.134    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 997
Number of extensions: 66
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 555
Length of database: 612
Length adjustment: 36
Effective length of query: 519
Effective length of database: 576
Effective search space:   298944
Effective search space used:   298944
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis