Alignments for a candidate for ilvI in Azospirillum lipoferum B510

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Azospirillum lipoferum B510

Align Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate WP_012975773.1 AZL_RS17220 glyoxylate carboligase

Query= curated2:O08353
         (599 letters)



>NCBI__GCF_000010725.1:WP_012975773.1
          Length = 589

 Score =  345 bits (886), Expect = 2e-99
 Identities = 186/554 (33%), Positives = 317/554 (57%), Gaps = 35/554 (6%)

Query: 1   MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLI-HLLTRHEQAAAHAADGYAR 59
           M  AEA ++ LE E +++ FG PG A+ PFY A+     + H+L RH + A+H A+GY R
Sbjct: 4   MTAAEAAVRVLEREGIDVCFGVPGAAINPFYAAMQRRGTMRHVLARHVEGASHMAEGYTR 63

Query: 60  A-SGKVGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLF 118
           A +G +GVCIGTSGP  T+++TG+ +A +DS P++ +TGQ P   +  + FQ I+   + 
Sbjct: 64  AEAGNIGVCIGTSGPAGTDMITGLYSAIADSIPILCITGQAPRARLHKEDFQAINVPDIA 123

Query: 119 MPIVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDIDKHPIPSKVK 178
            P+ K    + +  Q+P +F+ AF + ++GRPGPV IDLP DVQ  E++ D         
Sbjct: 124 KPVTKWALTVLEPAQVPYVFQQAFHLMRSGRPGPVLIDLPIDVQMAEIEFDDET------ 177

Query: 179 LIGYNPTTIGHP----RQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPV 234
              Y P  +  P     Q++KA+ + A+A+RP+++AGGG++ + A+++L++  ELL +PV
Sbjct: 178 ---YEPLPVYKPAATRAQVEKALAMFAAAERPLVVAGGGIINADASDKLVEFAELLGVPV 234

Query: 235 CTTLMGKGCISENHPLALGMVGMH-GTKPANYCLSESDVLISIGCRFSDRITGDIKSFAT 293
             TLMG G I ++HPL  GMVG+    +  N  + +SD ++ IG R+++R TG ++ +  
Sbjct: 235 IPTLMGWGTIPDDHPLMAGMVGLQTAHRYGNATMLKSDFVLGIGNRWANRHTGSVEVYTK 294

Query: 294 NAKIIHIDIDPAEIGKNVNVDVPIVGDAKLILKEVIKQLDYIINKDSKENNDKENISQWI 353
             K +H+DI+P +IG+    D+ IV DA   L   I+     + ++ K     +++  W+
Sbjct: 295 GRKFVHVDIEPTQIGRVFMPDLGIVSDAGAALDLFIE-----VAREWKAEGRFKDLGGWV 349

Query: 354 ENVNSLKKSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQNQMWMAHYF 413
           ++    K+S     D+D +P+KPQ++ +E+ +        ++T   T +G +Q+  A + 
Sbjct: 350 KDCQGRKRSMHRRSDFDQVPMKPQRVYQEMNSA-----FGQDTTYVTTIGLSQIAGAQFL 404

Query: 414 KTQTPRSFLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNI 473
               PR +++ G  G +G+  P+A+G +VA P  +++ ++GD  F    +EL   A++ +
Sbjct: 405 HVYKPRHWINCGQAGPLGWTLPAALGVRVADPKRRIVALSGDYDFQFMIEELAVGAQHKL 464

Query: 474 PVVICIFDNRTLGMVYQWQNLFYGKRQCSVNFG--GAP-------DFIKLAESYGIKARR 524
           P +  + +N  LG++ Q Q  F    Q  ++F    AP       D + +AE  G KA R
Sbjct: 465 PYIHVLVNNAYLGLIRQAQRAFQMDFQVQLSFENINAPEIGVYGVDHVAVAEGLGCKAIR 524

Query: 525 IESPNEINEALKEA 538
           +  P+ I EA  +A
Sbjct: 525 VTEPDRIQEAFTQA 538


Lambda     K      H
   0.319    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 803
Number of extensions: 40
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 599
Length of database: 589
Length adjustment: 37
Effective length of query: 562
Effective length of database: 552
Effective search space:   310224
Effective search space used:   310224
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis