Alignments for a candidate for asd in Sulfurimonas denitrificans DSM 1251

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd in Sulfurimonas denitrificans DSM 1251

Align aspartate semialdehyde dehydrogenase subunit (EC 1.2.1.11) (characterized)
to candidate WP_011372269.1 SUDEN_RS03330 aspartate-semialdehyde dehydrogenase

Query= metacyc::MONOMER-6564
         (346 letters)



>NCBI__GCF_000012965.1:WP_011372269.1
          Length = 344

 Score =  308 bits (790), Expect = 1e-88
 Identities = 170/337 (50%), Positives = 232/337 (68%), Gaps = 11/337 (3%)

Query: 6   HVAVVGATGAVGQQMLKTLEDRNFEMDTLTLLSSKRSAGTKVTFKGQELTVQEASPESFE 65
           +VAVVGA GAVG+++L  L++  F ++ L  L+S RS G  V + G+E+ ++E +   FE
Sbjct: 6   NVAVVGANGAVGEEILTILQEVKFPINKLIPLASSRSIGKTVMYAGREVAIKELTDTVFE 65

Query: 66  --GVNIALFSAGGSVSQALAPEAVKRGAIVIDNTSAFRMDENTPLVVPEVNEADL--HEH 121
              + IALFSAGGSVS   A  AV+ GA+VIDNTS FRMDEN PLVVPEVN  D+   + 
Sbjct: 66  EEDIEIALFSAGGSVSAQFAASAVRAGAVVIDNTSHFRMDENVPLVVPEVNPQDIAKWKK 125

Query: 122 NGIIANPNCSTIQMVAALEPIRKAYGLNKVIVSTYQAVSGAGNEAVKELYSQTQAILNKE 181
            GIIANPNCSTIQMV AL+P+ +AY + +V VSTYQA SGAG  A++EL +Q Q+    +
Sbjct: 126 TGIIANPNCSTIQMVQALKPLDEAYNIQRVDVSTYQATSGAGKSAMEELVTQMQSFFAFK 185

Query: 182 EIEPEIMPVKGDKKHYQIAFNAIPQIDKFQDNGYTFEEMKMINETKKIMHMPDLQVAATC 241
             E E+         ++IA N IPQIDKF DNGYT EEMKM+NET KIMH  ++ ++ATC
Sbjct: 186 LDEAEVKAFA-----HRIALNVIPQIDKFLDNGYTKEEMKMVNETTKIMHR-EIPLSATC 239

Query: 242 VRLPIQTGHSESVYIEIDRDDATVEDIKNLLKEAPGVTLQDDPSQQLYPMPADAVGKNDV 301
           VR+P   GH+E+V I+    +   + ++ LL++AP + + D+P + +YPMP+ A+ KN+ 
Sbjct: 240 VRVPTLRGHAEAVSIKF-ACEVNADKVRELLEKAPNIIVLDNPEKSIYPMPSIALEKNET 298

Query: 302 FVGRIRKDLDRANGFHLWVVSDNLLKGAAWNSVQIAE 338
           FVGRIR D       HL++V+DNL  GAA N+V+IA+
Sbjct: 299 FVGRIRVDNFDKTVLHLFIVADNLRVGAATNAVRIAQ 335


Lambda     K      H
   0.314    0.131    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 304
Number of extensions: 13
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 346
Length of database: 344
Length adjustment: 29
Effective length of query: 317
Effective length of database: 315
Effective search space:    99855
Effective search space used:    99855
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 49 (23.5 bits)

Align candidate WP_011372269.1 SUDEN_RS03330 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01296 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01296.hmm
# target sequence database:        /tmp/gapView.22026.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01296  [M=339]
Accession:   TIGR01296
Description: asd_B: aspartate-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   5.7e-138  445.6   5.8   6.6e-138  445.4   5.8    1.0  1  lcl|NCBI__GCF_000012965.1:WP_011372269.1  SUDEN_RS03330 aspartate-semialde


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000012965.1:WP_011372269.1  SUDEN_RS03330 aspartate-semialdehyde dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  445.4   5.8  6.6e-138  6.6e-138       1     337 [.       6     338 ..       6     340 .. 0.98

  Alignments for each domain:
  == domain 1  score: 445.4 bits;  conditional E-value: 6.6e-138
                                 TIGR01296   1 nvaivGatGavGqellkvLeernfpidklvllasersaGkkvkfkgkeleveeaekesfeg..idialf 67 
                                               nva+vGa GavG+e+l++L+e +fpi+kl++las+rs Gk v + g+e+ ++e++ ++fe+  i+ialf
  lcl|NCBI__GCF_000012965.1:WP_011372269.1   6 NVAVVGANGAVGEEILTILQEVKFPINKLIPLASSRSIGKTVMYAGREVAIKELTDTVFEEedIEIALF 74 
                                               79******************************************************9997666****** PP

                                 TIGR01296  68 saGgsvskefapkaakagviviDntsafrldedvPLvvpevnaeelkeakkkgiianPnCstiqlvvvL 136
                                               saGgsvs +fa++a++ag++viDnts fr+de+vPLvvpevn +++++ kk giianPnCstiq+v++L
  lcl|NCBI__GCF_000012965.1:WP_011372269.1  75 SAGGSVSAQFAASAVRAGAVVIDNTSHFRMDENVPLVVPEVNPQDIAKWKKTGIIANPNCSTIQMVQAL 143
                                               ********************************************************************* PP

                                 TIGR01296 137 kplkdeaklkrvvvstYqavsGaGkkgveeLknqtkavlegkekepeidalkakkfakqiafnaiplid 205
                                               kpl+++++++rv vstYqa sGaGk ++eeL  q+++ + +k  e+     + k+fa++ia n+ip+id
  lcl|NCBI__GCF_000012965.1:WP_011372269.1 144 KPLDEAYNIQRVDVSTYQATSGAGKSAMEELVTQMQSFFAFKLDEA-----EVKAFAHRIALNVIPQID 207
                                               ***************************************9998885.....6799************** PP

                                 TIGR01296 206 klkedGytkeelkllfetrkilgiedlkvsatcvrvPvftghsesvsiefekelsveevkelLkeapgv 274
                                               k+ ++Gytkee+k+++et ki++  ++++satcvrvP ++gh+e+vsi+f+ e+++++v+elL++ap +
  lcl|NCBI__GCF_000012965.1:WP_011372269.1 208 KFLDNGYTKEEMKMVNETTKIMH-REIPLSATCVRVPTLRGHAEAVSIKFACEVNADKVRELLEKAPNI 275
                                               ***********************.9******************************************** PP

                                 TIGR01296 275 vviddpsenlyptPleavgkdevfvgrirkDlskekglalfvvaDnlrkGaalnavqiaelli 337
                                               +v+d+p++  yp+P  a +k+e+fvgrir D +++  l+lf+vaDnlr+Gaa+nav+ia+++i
  lcl|NCBI__GCF_000012965.1:WP_011372269.1 276 IVLDNPEKSIYPMPSIALEKNETFVGRIRVDNFDKTVLHLFIVADNLRVGAATNAVRIAQKWI 338
                                               ***********************************************************9876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (339 nodes)
Target sequences:                          1  (344 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 7.24
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis