Alignments for a candidate for aroA in Pelobacter propionicus DSM 2379

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroA in Pelobacter propionicus DSM 2379

Align 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (characterized)
to candidate WP_011735260.1 PPRO_RS06685 3-phosphoshikimate 1-carboxyvinyltransferase

Query= BRENDA::A6TL04
         (425 letters)



>NCBI__GCF_000015045.1:WP_011735260.1
          Length = 431

 Score =  435 bits (1119), Expect = e-126
 Identities = 217/408 (53%), Positives = 293/408 (71%), Gaps = 1/408 (0%)

Query: 10  LEGKMTVPGDKSISHRAIMLSSISKGTSRVKGFLRGEDCLSTISCFRDLGIDIEDRGTEI 69
           L+G++ VPGDKSISHR+IML +I+ G + V+GFLRGED ++T++ FR +G+ I+D G  +
Sbjct: 12  LKGELVVPGDKSISHRSIMLGAIANGVTTVRGFLRGEDNMATMAAFRAMGVRIDDDGHLL 71

Query: 70  IIQGKGLHGLSEPLNVLDAGNSGTTIRLISGILAGQKFLTIVTGDASLRKRPMERIATPL 129
            I G+GLHGL EP +VLD GNSGT++RL++G+LAGQ F ++++GD  LRKRPM+R+  PL
Sbjct: 72  SIHGRGLHGLEEPGDVLDCGNSGTSMRLLTGLLAGQNFFSVLSGDQYLRKRPMKRVVEPL 131

Query: 130 RKMGAFIEGRDYGNLAPLVIRGGNLKGMDYASPVSSAQVKSAILLAGLYGEGDTIVREKI 189
            +MGA I GR  GNLAPL I GG L  + Y SPVSSAQ+KSAI+LAGLY +GDT VRE  
Sbjct: 132 SRMGARILGRAGGNLAPLAISGGTLNAIGYESPVSSAQIKSAIMLAGLYADGDTSVREPS 191

Query: 190 TSRDHTEKMLKGLGANIST-DQGVTRLGKSELYGQSIEVPGDISSAAFFMAGAAALPGSF 248
            SRDH+E+M    GA++ T   GVT  G  EL+ Q I VPGDISSAAFF+  A   P S 
Sbjct: 192 LSRDHSERMFALFGASLETFHNGVTVKGGIELHAQEIHVPGDISSAAFFIVAALITPDSE 251

Query: 249 LITEGVGLNPTRTGIIDVLRDMGGDIEIHNLRQSGGEEIGDIMIRGKKLYGTEIGKEIIP 308
           L+   VG+NPTRTGIIDVLR MGG IE+ + R+   E + DI++R  +L G  I  + +P
Sbjct: 252 LLIRNVGVNPTRTGIIDVLRSMGGSIELVDEREVSAEPVADILVRSSRLKGVRIEGQTVP 311

Query: 309 RLIDEIPVLAIIAATAEGKTIITGAEELKVKESNRITAMVTEMQKVGIKVTELPDGMEIE 368
           R IDE P + + AA AEG T I  A EL+VKE++RI+AM   ++ +G+ V E  +GM+I 
Sbjct: 312 RAIDEFPAICVAAACAEGTTSIRDARELRVKETDRISAMAVNLRTLGVTVDECDEGMDIT 371

Query: 369 GGQVITGGRVESYGDHRIAMAMAICGLFAQEPIKINDSQCIDISFPNF 416
           G + + GG  ES+GDHRIAM++++ GL + + +++ND  C+  SFPNF
Sbjct: 372 GVERLGGGVAESFGDHRIAMSLSVAGLVSADAVRVNDIDCVSTSFPNF 419


Lambda     K      H
   0.317    0.138    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 545
Number of extensions: 20
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 425
Length of database: 431
Length adjustment: 32
Effective length of query: 393
Effective length of database: 399
Effective search space:   156807
Effective search space used:   156807
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

Align candidate WP_011735260.1 PPRO_RS06685 (3-phosphoshikimate 1-carboxyvinyltransferase)
to HMM TIGR01356 (aroA: 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01356.hmm
# target sequence database:        /tmp/gapView.18926.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01356  [M=415]
Accession:   TIGR01356
Description: aroA: 3-phosphoshikimate 1-carboxyvinyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.1e-132  427.8   0.0   2.3e-132  427.6   0.0    1.0  1  lcl|NCBI__GCF_000015045.1:WP_011735260.1  PPRO_RS06685 3-phosphoshikimate 


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000015045.1:WP_011735260.1  PPRO_RS06685 3-phosphoshikimate 1-carboxyvinyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  427.6   0.0  2.3e-132  2.3e-132       1     412 [.      14     426 ..      14     429 .. 0.96

  Alignments for each domain:
  == domain 1  score: 427.6 bits;  conditional E-value: 2.3e-132
                                 TIGR01356   1 geikipgsKSishRalllaaLaegetvvtnlLkseDtlatlealrklGakveeekeelviegvgg..lk 67 
                                               ge+ +pg+KSishR+++l+a+a+g t+v+++L++eD  at+ a+r++G+++ +++  l i+g g   l+
  lcl|NCBI__GCF_000015045.1:WP_011735260.1  14 GELVVPGDKSISHRSIMLGAIANGVTTVRGFLRGEDNMATMAAFRAMGVRIDDDGHLLSIHGRGLhgLE 82 
                                               7889************************************************99*******998788** PP

                                 TIGR01356  68 epeaeldlgnsGttaRlltgvlalasgevvltgdeslkkRPierlveaLrelgaeieskeeegslPlai 136
                                               ep ++ld+gnsGt++Rlltg+la +++ +vl+gd+ l+kRP++r+ve+L+++ga+i  + + + +Plai
  lcl|NCBI__GCF_000015045.1:WP_011735260.1  83 EPGDVLDCGNSGTSMRLLTGLLAGQNFFSVLSGDQYLRKRPMKRVVEPLSRMGARILGRAGGNLAPLAI 151
                                               ********************************************************************* PP

                                 TIGR01356 137 sgplkggivelsgsaSsQyksalllaaplalqavtleivgeklisrpyieitLkllksfgveveeeder 205
                                               sg+   +i + s+  S+Q ksa++la+   l a++ + v e+ +sr++ e++ +l+++    +e  ++ 
  lcl|NCBI__GCF_000015045.1:WP_011735260.1 152 SGGTLNAIGYESPVSSAQIKSAIMLAG---LYADGDTSVREPSLSRDHSERMFALFGAS---LETFHN- 213
                                               **8777*********************...66778889999***********9999977...777665. PP

                                 TIGR01356 206 kivvkggqkykqkevevegDaSsAafflaaaaitge.evtvenlgenstqgdkaiiivLeemGadveve 273
                                                ++vkgg + + +e++v+gD+SsAaff++aa+it+  e+ ++n+g n+t+++  ii+vL++mG+++e+ 
  lcl|NCBI__GCF_000015045.1:WP_011735260.1 214 GVTVKGGIELHAQEIHVPGDISSAAFFIVAALITPDsELLIRNVGVNPTRTG--IIDVLRSMGGSIELV 280
                                               9******9966666**************************************..888************ PP

                                 TIGR01356 274 eqr........dvevegasklkgvkv.didvdsliDelptlavlaafAegetriknieelRvkEsdRia 333
                                               ++r        d+ v+ +s lkgv++  + v+++iDe+p++ v+aa+Aeg+t i++++elRvkE+dRi+
  lcl|NCBI__GCF_000015045.1:WP_011735260.1 281 DERevsaepvaDILVR-SSRLKGVRIeGQTVPRAIDEFPAICVAAACAEGTTSIRDARELRVKETDRIS 348
                                               **99999999999999.789******88***************************************** PP

                                 TIGR01356 334 aiaeeLeklGveveeledgllieGkkkelkgavvdtydDHRiamalavlglaaegeveiedaecvaksf 402
                                               a+a +L++lGv+v+e  +g+ i+G  ++l g+v+++++DHRiam+l+v+gl+ ++ v+++d  cv++sf
  lcl|NCBI__GCF_000015045.1:WP_011735260.1 349 AMAVNLRTLGVTVDECDEGMDITGV-ERLGGGVAESFGDHRIAMSLSVAGLVSADAVRVNDIDCVSTSF 416
                                               *************************.6****************************************** PP

                                 TIGR01356 403 PeFfevleql 412
                                               P+Ff +le++
  lcl|NCBI__GCF_000015045.1:WP_011735260.1 417 PNFFSLLERF 426
                                               *******987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (415 nodes)
Target sequences:                          1  (431 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 10.34
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis