Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (characterized)
to candidate WP_012044488.1 BBTA_RS20815 dihydroxy-acid dehydratase
Query= SwissProt::P9WKJ5 (575 letters) >NCBI__GCF_000015165.1:WP_012044488.1 Length = 574 Score = 546 bits (1407), Expect = e-160 Identities = 291/560 (51%), Positives = 372/560 (66%), Gaps = 8/560 (1%) Query: 16 DIKPR--SRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNEITPCNLSLDRLAN 73 DIK R SR VT+G E+A R L A+G+ +P +GVAS WNE PCN++L R A Sbjct: 6 DIKRRLPSRHVTEGPERAPHRSYLYAMGLTTAQIHQPFVGVASCWNEAAPCNIALMRQAQ 65 Query: 74 AVKEGVFSAGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVEVVMQAERLDGSVL 133 AVK+GV AGG P EF TI+V+DGI+MGH+GM SL SRE IADSVE+ ++ D V Sbjct: 66 AVKKGVAHAGGTPREFCTITVTDGIAMGHDGMRSSLPSRETIADSVELTIRGHAYDALVG 125 Query: 134 LAGCDKSLPGMLMAAARLDLAAVFLYAGSILPGRAKLSDGSERDVTIIDAFEAVGACSRG 193 LAGCDKSLPGM+MA RL++ ++F+Y GSILPG + + VT+ D FEAVG S G Sbjct: 126 LAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGNFR-----GQQVTVQDMFEAVGKHSVG 180 Query: 194 LMSRADVDAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAPPATDRRRDGFARR 253 MS D+D +ER CP GACG +TANTMA+ +EA+G++LP SA PA RD F Sbjct: 181 QMSDDDLDELERVACPSAGACGAQFTANTMATVSEAIGLALPYSAGAPAPYEIRDSFCMT 240 Query: 254 SGQAVVELLRRGITARDILTKEAFENAIAVVMAFGGSTNAVLHLLAIAHEANVALSLQDF 313 +G+ V+EL+ I RDI+T+ A ENA AVV A GGSTNA LHL AIAHEA + L D Sbjct: 241 AGEKVMELIAANIRPRDIVTRAALENAAAVVAASGGSTNAALHLPAIAHEAGIKFDLFDV 300 Query: 314 SRIGSGVPHLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDCLTVTGHTMAENLA 373 + I P++AD+KP GR+V D+ GG+P++MK LLD G L+GDCLTVTG T+AENL Sbjct: 301 AEIFKKTPYIADLKPGGRYVAKDMFEAGGIPLLMKTLLDHGYLNGDCLTVTGRTIAENLK 360 Query: 374 AITPPDPDGKVLRALANPIHPSGGITILHGSLAPEGAVVKTAGFDSDVFEGTARVFDGER 433 + +P V+R+ PI +GG+ L G+LAPEGA+VK AG + F G AR FD E Sbjct: 361 GV-KWNPHQDVVRSADQPITATGGVVGLKGNLAPEGAIVKVAGMSNLKFSGPARCFDREE 419 Query: 434 AALDALEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGKDVLLLTDGRFSGG 493 A +A++ T GD +VIRYEGP+GGPGMREML T A+ G G+G + L+TDGRFSG Sbjct: 420 DAFEAVQKRTYREGDVIVIRYEGPRGGPGMREMLQTTAALTGQGMGGKIALITDGRFSGA 479 Query: 494 TTGLCVGHIAPEAVDGGPIALLRNGDRIRLDVAGRVLDVLADPAEFASRQQDFSPPPPRY 553 T G C+GHI PEA GGPIAL+ +GD I +D LDV AE A+R+ ++P ++ Sbjct: 480 TRGFCIGHIGPEAAIGGPIALVEDGDIIEIDAVAGRLDVKLSDAELAARKTKWTPRETQH 539 Query: 554 TTGVLSKYVKLVSSAAVGAV 573 T+G L KY + V A GAV Sbjct: 540 TSGALWKYAQQVGPAVAGAV 559 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 968 Number of extensions: 51 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 575 Length of database: 574 Length adjustment: 36 Effective length of query: 539 Effective length of database: 538 Effective search space: 289982 Effective search space used: 289982 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate WP_012044488.1 BBTA_RS20815 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.2095.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.6e-212 692.8 2.0 1.8e-212 692.6 2.0 1.0 1 lcl|NCBI__GCF_000015165.1:WP_012044488.1 BBTA_RS20815 dihydroxy-acid dehy Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000015165.1:WP_012044488.1 BBTA_RS20815 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 692.6 2.0 1.8e-212 1.8e-212 1 541 [. 24 560 .. 24 562 .. 0.99 Alignments for each domain: == domain 1 score: 692.6 bits; conditional E-value: 1.8e-212 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 ++r+ l+a+Gl+ +++++P+++v+++++e +P+++ l a++vk+++ +aGg++ ef ti+v+DGiam lcl|NCBI__GCF_000015165.1:WP_012044488.1 24 PHRSYLYAMGLTTAQIHQPFVGVASCWNEAAPCNIALMRQAQAVKKGVAHAGGTPREFCTITVTDGIAM 92 699****************************************************************** PP TIGR00110 70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138 gh+Gm+ sLpsre iaDsve +++ha+Dalv ++ CDk +PGm+ma++rln+P+i+++GG++ +g+++ lcl|NCBI__GCF_000015165.1:WP_012044488.1 93 GHDGMRSSLPSRETIADSVELTIRGHAYDALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGNFR 161 ********************************************************************* PP TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207 ++++++ d+feavg+ + g++s+++l+e+er+acP+ag+C+ ftan+ma+++ea+Gl+lP+s+ ++a lcl|NCBI__GCF_000015165.1:WP_012044488.1 162 -GQQVTVQDMFEAVGKHSVGQMSDDDLDELERVACPSAGACGAQFTANTMATVSEAIGLALPYSAGAPA 229 .9******************************************************************* PP TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsld 276 + + +++ ++g++++el+ ni+Prdi+t++a+ena +++ a GGstn+ Lhl+aia+eag+k++l lcl|NCBI__GCF_000015165.1:WP_012044488.1 230 PYEIRDSFCMTAGEKVMELIAANIRPRDIVTRAALENAAAVVAASGGSTNAALHLPAIAHEAGIKFDLF 298 ********************************************************************* PP TIGR00110 277 dfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlrv 345 d+ ++ +k+P +a+lkP+g++v +d+ +aGG++ ++k+l +g+l+ d+ltvtG+t+ae+l+ vk + lcl|NCBI__GCF_000015165.1:WP_012044488.1 299 DVAEIFKKTPYIADLKPGGRYVAKDMFEAGGIPLLMKTLLDHGYLNGDCLTVTGRTIAENLKGVKWN-P 366 ******************************************************************9.8 PP TIGR00110 346 dqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeG 414 +qdv+rs d+p++++gg+ LkGnla+eGa+vk+ag+++ lkf Gpa+ f+ ee+a ea+ ++ +eG lcl|NCBI__GCF_000015165.1:WP_012044488.1 367 HQDVVRSADQPITATGGVVGLKGNLAPEGAIVKVAGMSN--LKFSGPARCFDREEDAFEAVQKRTYREG 433 9**************************************..**************************** PP TIGR00110 415 dvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialve 483 dv+viryeGP+GgPGmremL+ t+al+g G+g k+aLitDGrfsG+trG++iGh+ Peaa gG+ialve lcl|NCBI__GCF_000015165.1:WP_012044488.1 434 DVIVIRYEGPRGGPGMREMLQTTAALTGQGMGGKIALITDGRFSGATRGFCIGHIGPEAAIGGPIALVE 502 ********************************************************************* PP TIGR00110 484 dGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavl 541 dGD+i+iD+ + +ld+++s++ela+r+ k++++e+++++gaL kya+ v a Gav+ lcl|NCBI__GCF_000015165.1:WP_012044488.1 503 DGDIIEIDAVAGRLDVKLSDAELAARKTKWTPRETQHTSGALWKYAQQVGPAVAGAVT 560 ********************************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (574 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 8.81 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory