Align Branched-chain amino acid aminotransferase (EC 2.6.1.42) (characterized)
to candidate WP_012041978.1 BBTA_RS08080 branched-chain amino acid aminotransferase
Query= reanno::Cup4G11:RR42_RS25890 (363 letters) >NCBI__GCF_000015165.1:WP_012041978.1 Length = 359 Score = 439 bits (1130), Expect = e-128 Identities = 217/356 (60%), Positives = 263/356 (73%), Gaps = 1/356 (0%) Query: 6 TFSLEPNPNALDAATRDALMRDPAFGRVFTDHMVTITWREGQGWQDAKVTARKPFSIDPA 65 TF ++P N R A + +P FGRVFTDHM + + + +GW A+V +R F +DPA Sbjct: 4 TFDIKPTTNPTSDQERAAKLANPGFGRVFTDHMAVVQFSQDKGWHSARVESRANFPLDPA 63 Query: 66 CSVLHYGQEIFEGMKAYRGADGAVTLFRPLENARRFQASAKRMAMPALPESLFLEAIEQL 125 +VLHY QEIFEG+KAY+ DG V LFRP NA+RF SA+RMAM LP+++F+EA+EQL Sbjct: 64 AAVLHYAQEIFEGLKAYKRDDGGVNLFRPDANAQRFWNSAERMAMAQLPQNVFIEAVEQL 123 Query: 126 VRIDQAWVPHGSGSLYLRPFMFANEVFLGIKPASEFIFCVIACPVGPYFKGGDKAVSVWV 185 VRID+AW+P G GSLYLRPFM ANEVFLG+KP++E+IF VIA PVG YFKGG VS+WV Sbjct: 124 VRIDRAWIPGGEGSLYLRPFMIANEVFLGVKPSAEYIFAVIASPVGSYFKGGPAPVSIWV 183 Query: 186 SENYTRAAPGGTGEAKCGGNYAGSLVAQNEATANGCDQVVFLDAAEHRWVEELGGMNIFF 245 SENYTRAA GGTG KCGGNYA SL AQ EA A+GCDQVVFLDA E R+VEELGGMN+FF Sbjct: 184 SENYTRAAVGGTGGVKCGGNYAASLKAQAEAIAHGCDQVVFLDAIERRYVEELGGMNVFF 243 Query: 246 VMDDGTLVTPPLSGSILPGITRASVIELAREMGMVVEERRYSYPEWEADAKSGRLAEAFV 305 V DDG+L TPPL G+ILPGITR S+I+LAR+ G V E Y+ +W ADAKSG+L EAF Sbjct: 244 VFDDGSLSTPPL-GTILPGITRDSIIKLARDAGREVREEAYAIDQWRADAKSGKLKEAFA 302 Query: 306 CGTAATLVAIGEVRSARTRFAIGNGTAGNTVKVLRDRLVEIQRNQAAGPAGWVHHV 361 CGTAA + IG+VRSA I G AG LR +LV+IQ ++A P W+ V Sbjct: 303 CGTAAVISPIGKVRSASGDVLINGGEAGPVAMGLRKQLVDIQYGRSADPHNWIRDV 358 Lambda K H 0.321 0.135 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 447 Number of extensions: 16 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 363 Length of database: 359 Length adjustment: 29 Effective length of query: 334 Effective length of database: 330 Effective search space: 110220 Effective search space used: 110220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 49 (23.5 bits)
Align candidate WP_012041978.1 BBTA_RS08080 (branched-chain amino acid aminotransferase)
to HMM TIGR01123 (ilvE: branched-chain amino acid aminotransferase (EC 2.6.1.42))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01123.hmm # target sequence database: /tmp/gapView.22011.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01123 [M=313] Accession: TIGR01123 Description: ilvE_II: branched-chain amino acid aminotransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.8e-127 408.7 0.0 7.9e-127 408.5 0.0 1.0 1 lcl|NCBI__GCF_000015165.1:WP_012041978.1 BBTA_RS08080 branched-chain amin Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000015165.1:WP_012041978.1 BBTA_RS08080 branched-chain amino acid aminotransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 408.5 0.0 7.9e-127 7.9e-127 1 312 [. 47 357 .. 47 358 .. 0.98 Alignments for each domain: == domain 1 score: 408.5 bits; conditional E-value: 7.9e-127 TIGR01123 1 WdeaelaseaeleldegsavlhYgqevfeGlkayRtadGkillfRpdanakRlrrsaerlllPeleeel 69 W++a+++s a++ ld+++avlhY+qe+feGlkay+ +dG ++lfRpdana+R+ +saer+++ +l++++ lcl|NCBI__GCF_000015165.1:WP_012041978.1 47 WHSARVESRANFPLDPAAAVLHYAQEIFEGLKAYKRDDGGVNLFRPDANAQRFWNSAERMAMAQLPQNV 115 ********************************************************************* PP TIGR01123 70 flealkqlvkadkdwvpkakseasLYlRPfliatednlGvkaakeylflvlasPvGaYfkgglapvsif 138 f+ea++qlv++d++w+p ++e sLYlRPf+ia e lGvk++ ey+f v+asPvG+Yfkgg apvsi+ lcl|NCBI__GCF_000015165.1:WP_012041978.1 116 FIEAVEQLVRIDRAWIP--GGEGSLYLRPFMIANEVFLGVKPSAEYIFAVIASPVGSYFKGGPAPVSIW 182 *****************..788*********************************************** PP TIGR01123 139 veteyvRaapkGtGavkvgGnYaasllaqkkaaeqglddvvyldpvekkkieevGaaniflitkdgelv 207 v+++y+Raa +GtG+vk+gGnYaasl+aq++a ++g+d+vv+ld++e++++ee+G++n+f++ +dg+l lcl|NCBI__GCF_000015165.1:WP_012041978.1 183 VSENYTRAAVGGTGGVKCGGNYAASLKAQAEAIAHGCDQVVFLDAIERRYVEELGGMNVFFVFDDGSLS 251 *****************************************************************9999 PP TIGR01123 208 ttplsesiLegvtresllelakdlgleveereiaidelkaaveaGei..vfacGtaavitPvgelkieg 274 t+pl + iL+g+tr+s+++la+d+g ev+e+ aid+++a +++G++ +facGtaavi+P+g+++ lcl|NCBI__GCF_000015165.1:WP_012041978.1 252 TPPL-GTILPGITRDSIIKLARDAGREVREEAYAIDQWRADAKSGKLkeAFACGTAAVISPIGKVRSAS 319 9887.78**************************************9999******************** PP TIGR01123 275 kevevkseevGevtkklrdeltdiqyGkledkegWive 312 v ++ +e G+v++ lr++l+diqyG+ +d+++Wi lcl|NCBI__GCF_000015165.1:WP_012041978.1 320 GDVLINGGEAGPVAMGLRKQLVDIQYGRSADPHNWIRD 357 ***********************************976 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (313 nodes) Target sequences: 1 (359 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 9.28 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory