Align Putative acetolactate synthase large subunit IlvX; ALS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; AHAS (uncharacterized)
to candidate WP_012044691.1 BBTA_RS21905 acetolactate synthase large subunit
Query= curated2:O53554 (515 letters) >NCBI__GCF_000015165.1:WP_012044691.1 Length = 553 Score = 509 bits (1311), Expect = e-148 Identities = 279/517 (53%), Positives = 353/517 (68%), Gaps = 8/517 (1%) Query: 2 NGAQALINTLVDGGVDVCFANPGTSEMHFVAALDAVPRMRGMLTLFEGVATGAADGYARI 61 NGAQALI TL + GV VCF NPGTSEMHFVAALD+ MR +L LFEGVATGAADGYAR+ Sbjct: 3 NGAQALIRTLANAGVRVCFTNPGTSEMHFVAALDSAAEMRAVLCLFEGVATGAADGYARM 62 Query: 62 AGRPAAVLLHLGPGLGNGLANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDAVAGT 121 G PAA LLHLG GLGNGLANLHNAR+ +VP+V +VGDHATYH KYDA L+SDI+ VA Sbjct: 63 TGMPAATLLHLGCGLGNGLANLHNARKGKVPVVNIVGDHATYHVKYDAQLQSDIETVARN 122 Query: 122 VS-GWVRRTEAAADVGADAEAAIAASRS-GSQIATLILPADVCWSDGA--HAAAGVPAQA 177 VS +V ++ +++ DA AA+ A+ S Q+ATLILPADV W +G A + Sbjct: 123 VSPNFVWTSQVTSELCRDAVAAVKAACSWPGQVATLILPADVSWGEGGVPEPAPVIEPPP 182 Query: 178 AAAPVDVGPVAGVLRSGEPAMMLIGGDATRGPGLTAAARIVQATGARWLCETFPTCLERG 237 AA V VA +++G + +L+GG A L AAARI G + ETFPT ++RG Sbjct: 183 AADDATVTEVADTIKAGGRSAILLGGTALHEESLLAAARIAATCGVKVYAETFPTRMQRG 242 Query: 238 AGIPAVERLAYFAEGAAAQLDGVKHLVLAGARSPVSFFAYPGMPSDLVPAGCEVHVLAEP 297 AG+PAV+R+AY AE A+ QL + L+L A++PVSFFAYPG S L P C+V L+ P Sbjct: 243 AGLPAVDRIAYLAEMASIQLREIDTLILVDAKAPVSFFAYPGKKSYLAPDNCKVLTLSTP 302 Query: 298 G-GAADALAALADEV-APGTVAPVAGASRPQLPTGD-LTSVSAADVVGALLPERAIVVDE 354 AA +L LA + A P+A +RP P G+ LT+ VG LLPE AI++DE Sbjct: 303 AQDAAGSLKKLAAALQAESATPPLAPPARPGRPRGNKLTAEKVCKAVGHLLPENAILIDE 362 Query: 355 SNTCGVLLPQATAGAPAHDWLTLTGGAIGYGIPAAVGAAVAAPDRPVLCLESDGSAMYTI 414 + + G++L + TAGAP HD +TLTGGAIG G+P A+GAA+A PDRPV+ L DGS+MYTI Sbjct: 363 AISSGLMLSKFTAGAPRHDLITLTGGAIGQGLPNAIGAAIACPDRPVIALIGDGSSMYTI 422 Query: 415 SGLWSQARENLDVTTVIYNNGAYDILRIELQRVGAGSDPGPKALDLLDISRPTMDFVKIA 474 LW+ ARE L+VT +I+NN +Y +L +EL+RVGA GPKA LD+ P + F +IA Sbjct: 423 QSLWTIAREQLNVTAIIFNNASYSVLNVELERVGA-ERIGPKARAQLDLHGPVLKFAQIA 481 Query: 475 EGMGVPARRVTTCEEFADALRAAFAEPGPHLIDVVVP 511 +GMGV A R TT +E DAL +A PGPHLI+ VVP Sbjct: 482 DGMGVRATRATTPDELTDALASALKRPGPHLIEAVVP 518 Lambda K H 0.318 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 651 Number of extensions: 23 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 515 Length of database: 553 Length adjustment: 35 Effective length of query: 480 Effective length of database: 518 Effective search space: 248640 Effective search space used: 248640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory