Align aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) (characterized)
to candidate WP_011782622.1 MVAN_RS27655 aspartate-semialdehyde dehydrogenase
Query= BRENDA::P9WNX5 (345 letters) >NCBI__GCF_000015305.1:WP_011782622.1 Length = 344 Score = 575 bits (1483), Expect = e-169 Identities = 297/341 (87%), Positives = 310/341 (90%) Query: 5 IGIVGATGQVGQVMRTLLDERDFPASAVRFFASARSQGRKLAFRGQEIEVEDAETADPSG 64 IG+VGATGQVGQVMRTLL ERDFPA+ VRFFASARS G+KL FRGQEIEVEDA TADPSG Sbjct: 4 IGVVGATGQVGQVMRTLLAERDFPATGVRFFASARSAGKKLGFRGQEIEVEDASTADPSG 63 Query: 65 LDIALFSAGSAMSKVQAPRFAAAGVTVIDNSSAWRKDPDVPLVVSEVNFERDAHRRPKGI 124 LDIALFSAG+ MS+VQAPRFA AG VIDNSSAWRKDPDVPLVVSEVNFERDA RPKGI Sbjct: 64 LDIALFSAGATMSRVQAPRFAGAGAVVIDNSSAWRKDPDVPLVVSEVNFERDAGNRPKGI 123 Query: 125 IANPNCTTMAAMPVLKVLHDEARLVRLVVSSYQAVSGSGLAGVAELAEQARAVIGGAEQL 184 IANPNCTTMAAMPVLK LHDEA L RL+VSSYQAVSGSGLAGV ELA QARAVI G EQL Sbjct: 124 IANPNCTTMAAMPVLKPLHDEAGLTRLIVSSYQAVSGSGLAGVEELAGQARAVIDGVEQL 183 Query: 185 VYDGGALEFPPPNTYVAPIAFNVVPLAGSLVDDGSGETDEDQKLRFESRKILGIPDLLVS 244 V+DG AL+FPPPN YVAPIAFNVVPLAGSLVDDGSGETDEDQKLR ESRKILGIP+L VS Sbjct: 184 VHDGSALQFPPPNKYVAPIAFNVVPLAGSLVDDGSGETDEDQKLRNESRKILGIPELAVS 243 Query: 245 GTCVRVPVFTGHSLSINAEFAQPLSPERARELLDGATGVQLVDVPTPLAAAGVDESLVGR 304 GTCVRVPVFTGHSLSINAEF+QPLS RA+E+L GA GV LVDVPTPLAAAG D+SLVGR Sbjct: 244 GTCVRVPVFTGHSLSINAEFSQPLSVARAKEILAGAAGVTLVDVPTPLAAAGADDSLVGR 303 Query: 305 IRRDPGVPDGRGLALFVSGDNLRKGAALNTIQIAELLTADL 345 IRRDPGVPD RGLALFVSGDNLRKGAALNTIQIAELL A L Sbjct: 304 IRRDPGVPDDRGLALFVSGDNLRKGAALNTIQIAELLAAQL 344 Lambda K H 0.317 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 539 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 345 Length of database: 344 Length adjustment: 29 Effective length of query: 316 Effective length of database: 315 Effective search space: 99540 Effective search space used: 99540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
Align candidate WP_011782622.1 MVAN_RS27655 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01296 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01296.hmm # target sequence database: /tmp/gapView.2752.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01296 [M=339] Accession: TIGR01296 Description: asd_B: aspartate-semialdehyde dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.1e-148 479.9 0.0 2.4e-148 479.7 0.0 1.0 1 lcl|NCBI__GCF_000015305.1:WP_011782622.1 MVAN_RS27655 aspartate-semialdeh Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000015305.1:WP_011782622.1 MVAN_RS27655 aspartate-semialdehyde dehydrogenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 479.7 0.0 2.4e-148 2.4e-148 1 338 [. 3 342 .. 3 343 .. 0.99 Alignments for each domain: == domain 1 score: 479.7 bits; conditional E-value: 2.4e-148 TIGR01296 1 nvaivGatGavGqellkvLeernfpidklvllasersaGkkvkfkgkeleveeaekesfegidialfsa 69 ++++vGatG+vGq+++++L+er+fp+ ++++as+rsaGkk+ f+g+e+eve+a++++++g+dialfsa lcl|NCBI__GCF_000015305.1:WP_011782622.1 3 RIGVVGATGQVGQVMRTLLAERDFPATGVRFFASARSAGKKLGFRGQEIEVEDASTADPSGLDIALFSA 71 599****************************************************************** PP TIGR01296 70 GgsvskefapkaakagviviDntsafrldedvPLvvpevnaeelkeakkkgiianPnCstiqlvvvLkp 138 G+++s+++ap++a ag++viDn+sa+r+d+dvPLvv+evn+e+++ ++kgiianPnC+t+++++vLkp lcl|NCBI__GCF_000015305.1:WP_011782622.1 72 GATMSRVQAPRFAGAGAVVIDNSSAWRKDPDVPLVVSEVNFERDAGNRPKGIIANPNCTTMAAMPVLKP 140 ********************************************************************* PP TIGR01296 139 lkdeaklkrvvvstYqavsGaGkkgveeLknqtkavlegkekepeida....lkakkfakqiafnaipl 203 l+dea+l r++vs+YqavsG+G +gveeL+ q++av++g e+ +++++ ++++k++++iafn++pl lcl|NCBI__GCF_000015305.1:WP_011782622.1 141 LHDEAGLTRLIVSSYQAVSGSGLAGVEELAGQARAVIDGVEQLVHDGSalqfPPPNKYVAPIAFNVVPL 209 ********************************************9996678899*************** PP TIGR01296 204 idklkedG..ytkeelkllfetrkilgiedlkvsatcvrvPvftghsesvsiefekelsveevkelLke 270 +++l++dG +t+e++kl++e+rkilgi++l vs+tcvrvPvftghs+s+++ef+++lsv ++ke+L + lcl|NCBI__GCF_000015305.1:WP_011782622.1 210 AGSLVDDGsgETDEDQKLRNESRKILGIPELAVSGTCVRVPVFTGHSLSINAEFSQPLSVARAKEILAG 278 *******9889********************************************************** PP TIGR01296 271 apgvvviddpsenlyptPleavgkdevfvgrirkD..lskekglalfvvaDnlrkGaalnavqiaelli 337 a gv+++d+p tPl+a+g d+++vgrir+D +++++glalfv++DnlrkGaaln++qiaell lcl|NCBI__GCF_000015305.1:WP_011782622.1 279 AAGVTLVDVP------TPLAAAGADDSLVGRIRRDpgVPDDRGLALFVSGDNLRKGAALNTIQIAELLA 341 *********9......**************************************************997 PP TIGR01296 338 k 338 + lcl|NCBI__GCF_000015305.1:WP_011782622.1 342 A 342 5 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (339 nodes) Target sequences: 1 (344 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.25 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory