Align homoserine dehydrogenase (EC 1.1.1.3) (characterized)
to candidate WP_011781503.1 MVAN_RS21825 homoserine dehydrogenase
Query= BRENDA::P46806 (441 letters) >NCBI__GCF_000015305.1:WP_011781503.1 Length = 438 Score = 689 bits (1778), Expect = 0.0 Identities = 366/438 (83%), Positives = 396/438 (90%), Gaps = 2/438 (0%) Query: 3 SDERTVGVAVLGLGNVGSEVVRIIEGSADDLAARIGAPLMLRGIGVRRVAVDRGVPVDLL 62 SDE +GVAVLGLGNVGSEVVRIIE SA DL ARIGAPL++RGIGVRRV DRGVPV+LL Sbjct: 2 SDE--IGVAVLGLGNVGSEVVRIIEESAADLTARIGAPLVIRGIGVRRVDADRGVPVELL 59 Query: 63 TDNIEELVSRADVDIVVEVMGPVELSRKAILSALEHGKSVVTANKALLAASTGELAQAAE 122 TD+IE LVSR DVDIVVE+MGPVE +RKAIL+ALE GKSVVTANKAL+A S G+LA+AAE Sbjct: 60 TDDIEALVSRDDVDIVVELMGPVEPARKAILAALEQGKSVVTANKALMAVSAGKLAEAAE 119 Query: 123 SAHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIVNGTTNYILSAMDSTGADYDSAL 182 AHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIVNGTTNYILS MDSTGADY SAL Sbjct: 120 KAHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIVNGTTNYILSEMDSTGADYASAL 179 Query: 183 AGARALGYAEADPTADVEGHDAAAKAAILASIAFHTRVTADDVYREGITKITPADFVSAR 242 A A ALGYAEADPTADVEG DAAAKAAILASIAFHTRVTADDVYREGITK++ ADF SAR Sbjct: 180 ADASALGYAEADPTADVEGWDAAAKAAILASIAFHTRVTADDVYREGITKVSAADFASAR 239 Query: 243 ALGCTIKLLFICERITAADGQQRVSARVYPALVPMSHPLATVSGAFNAVVVEAEAAGRLM 302 ALGCTIKLL ICER+T G+Q+VSARVYPALVP+ HPLA+V+GAFNAVVVEAEAAGRLM Sbjct: 240 ALGCTIKLLAICERLTTGKGKQQVSARVYPALVPLEHPLASVNGAFNAVVVEAEAAGRLM 299 Query: 303 FYGQGAGGAPTASAVTGDLVMAARNRVLGSRGPKESKYAQLPMETIGFISTRYYVSMNVA 362 FYGQGAGGAPTASAV GDLVMAARNRV G RGP+ESKYA+LP+ IG I TRYYV+MNVA Sbjct: 300 FYGQGAGGAPTASAVLGDLVMAARNRVQGGRGPRESKYAKLPVAPIGVIPTRYYVNMNVA 359 Query: 363 DKPGVLSGVAAEFAKREVSIAEVRQEGVVDDGGRRVGARIVVVTHGATDAALSETVDALA 422 D+PGVLS V+AEF+KREVSIAEVRQEG+VDD G+R GARIVVVTH A DAALSETV ALA Sbjct: 360 DRPGVLSAVSAEFSKREVSIAEVRQEGMVDDEGQRCGARIVVVTHQAKDAALSETVAALA 419 Query: 423 DLDVVQGVTSVLRLEGIS 440 DLDVVQ + SVLR+EG S Sbjct: 420 DLDVVQSINSVLRMEGTS 437 Lambda K H 0.317 0.133 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 589 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 441 Length of database: 438 Length adjustment: 32 Effective length of query: 409 Effective length of database: 406 Effective search space: 166054 Effective search space used: 166054 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory