Align Shikimate kinase; SK; EC 2.7.1.71 (uncharacterized)
to candidate WP_011802318.1 PNAP_RS14700 helix-turn-helix domain-containing protein
Query= curated2:A5GQN5 (195 letters) >NCBI__GCF_000015505.1:WP_011802318.1 Length = 324 Score = 90.9 bits (224), Expect = 2e-23 Identities = 54/162 (33%), Positives = 91/162 (56%), Gaps = 3/162 (1%) Query: 21 LVGMMGSGKSTVGPLLAKALGYRFLDADAVISQAAGCSIPEIFERDGEEGFRQLERQVLQ 80 L+G+ G+GKST+G LLA+ LG+ F++ I + AGCS+ EI G +R+ ER+ L+ Sbjct: 155 LIGLRGAGKSTLGALLAEDLGFPFVELSREIEKFAGCSVSEIQALYGMNAYRRYERRALE 214 Query: 81 QLSQ-WHSLVVATGGGIVTVPANWGE-LRQGVVIWLDVAEEELMRRLQADPGGRPLLAGD 138 + Q + V+AT GG+V+ PA + + L +WL E+ M+R+ A RP+ A Sbjct: 215 ETIQIYPEAVIATPGGLVSDPATFNQLLSHCTTVWLQADPEDHMKRVLAQGDLRPMAASK 274 Query: 139 DPAGRLHGLLEKRQPLYGQADLRVSAQGEGASDISERILQQL 180 L G+L R Y +A +V + + ++S + L+++ Sbjct: 275 QAMEDLKGILAGRAAFYSKAQYKVDTSTQ-SLELSFQALREM 315 Lambda K H 0.316 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 165 Number of extensions: 9 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 195 Length of database: 324 Length adjustment: 24 Effective length of query: 171 Effective length of database: 300 Effective search space: 51300 Effective search space used: 51300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory