Alignments for a candidate for serB in Polaromonas naphthalenivorans CJ2

GapMind for Amino acid biosynthesis

Alignments for a candidate for serB in Polaromonas naphthalenivorans CJ2

Align Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 (characterized)
to candidate WP_011801935.1 PNAP_RS12765 phosphoserine phosphatase SerB

Query= SwissProt::Q12A06
         (236 letters)



>NCBI__GCF_000015505.1:WP_011801935.1
          Length = 236

 Score =  399 bits (1026), Expect = e-116
 Identities = 197/236 (83%), Positives = 220/236 (93%)

Query: 1   MQPTEISPGLVVNVATPDLKLSDFKLIAFDMDSTLINIECVDEIADAAGRKAEVAAITEA 60
           M P E SPGLVVN+ATPDLKL DFKLIAFDMDSTLINIECVDEIADA GRKAEVAAITEA
Sbjct: 1   MNPIEHSPGLVVNIATPDLKLRDFKLIAFDMDSTLINIECVDEIADAVGRKAEVAAITEA 60

Query: 61  AMRGEISDYKESLRQRVALLKGVSVASMDEVYRTRLRLNPGAARLVQACKDAGLKVLLVS 120
           AMRGEI+D+K+SLR+RVALLKGVS+ASMDEVYR RL+LNPGAA LV+ACKDAG+K+LLVS
Sbjct: 61  AMRGEITDFKDSLRRRVALLKGVSMASMDEVYRERLKLNPGAAELVRACKDAGMKILLVS 120

Query: 121 GGFTFFTDRIRDELGIDYTRSNVLETTDGLLTGRMVDQPWGDICDGEEKRKMLLETCGQL 180
           GGFT+FTDR++  L ID+TRSNVLE  DGLLTG+M+DQ WGDICDGEEKR+ML++TCGQL
Sbjct: 121 GGFTYFTDRVKGLLDIDFTRSNVLEVRDGLLTGKMIDQSWGDICDGEEKRRMLIQTCGQL 180

Query: 181 GISPRQAIAMGDGANDLPMMGEAGLSVAYHAKPRVREQAMVAINEGGLDRLLELVK 236
           GI+P QAIAMGDGANDLPMMG AGLSVAYHAKP+VREQAMVAIN GGLDRLLEL++
Sbjct: 181 GINPLQAIAMGDGANDLPMMGAAGLSVAYHAKPKVREQAMVAINAGGLDRLLELMR 236


Lambda     K      H
   0.319    0.136    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 284
Number of extensions: 4
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 236
Length of database: 236
Length adjustment: 23
Effective length of query: 213
Effective length of database: 213
Effective search space:    45369
Effective search space used:    45369
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

Align candidate WP_011801935.1 PNAP_RS12765 (phosphoserine phosphatase SerB)
to HMM TIGR00338 (serB: phosphoserine phosphatase SerB (EC 3.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00338.hmm
# target sequence database:        /tmp/gapView.26933.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00338  [M=219]
Accession:   TIGR00338
Description: serB: phosphoserine phosphatase SerB
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    2.5e-70  222.5   0.3    2.9e-70  222.3   0.3    1.0  1  lcl|NCBI__GCF_000015505.1:WP_011801935.1  PNAP_RS12765 phosphoserine phosp


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000015505.1:WP_011801935.1  PNAP_RS12765 phosphoserine phosphatase SerB
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  222.3   0.3   2.9e-70   2.9e-70      11     218 ..      21     234 ..      12     235 .. 0.95

  Alignments for each domain:
  == domain 1  score: 222.3 bits;  conditional E-value: 2.9e-70
                                 TIGR00338  11 lkkkklvvfDlDstlieeEvIdeiaklaGveeeVseiTerAmrgel.dFkeslreRvkllkglpvellk 78 
                                               l+  kl+ fD+Dstli++E++deia ++G + eV++iTe Amrge+ dFk+slr Rv+llkg+++  ++
  lcl|NCBI__GCF_000015505.1:WP_011801935.1  21 LRDFKLIAFDMDSTLINIECVDEIADAVGRKAEVAAITEAAMRGEItDFKDSLRRRVALLKGVSMASMD 89 
                                               56779****************************************769******************999 PP

                                 TIGR00338  79 kv.eeklelteGveelvkkLkekgykvaviSGgFdlvaeklkekLgldavfaNrLevedgkltGkve.. 144
                                               +v +e+l+l +G+ elv++ k++g+k+ ++SGgF+++++++k  L++d+  +N Lev+dg ltGk+   
  lcl|NCBI__GCF_000015505.1:WP_011801935.1  90 EVyRERLKLNPGAAELVRACKDAGMKILLVSGGFTYFTDRVKGLLDIDFTRSNVLEVRDGLLTGKMIdq 158
                                               983589**********************************************************97544 PP

                                 TIGR00338 145 ..geivdesakaktllkllekegislektvavGDGanDlsmikaAglgiafnakpvlkekadiviekkd 211
                                                 g+i d ++k ++l + + + gi++ + +a+GDGanDl+m+ aAgl +a++akp+++e+a ++i+   
  lcl|NCBI__GCF_000015505.1:WP_011801935.1 159 swGDICDGEEKRRMLIQTCGQLGINPLQAIAMGDGANDLPMMGAAGLSVAYHAKPKVREQAMVAINAGG 227
                                               4499***************************************************************99 PP

                                 TIGR00338 212 ltdilel 218
                                               l  +lel
  lcl|NCBI__GCF_000015505.1:WP_011801935.1 228 LDRLLEL 234
                                               9999887 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (219 nodes)
Target sequences:                          1  (236 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 8.29
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis