Align NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 (characterized)
to candidate WP_041376665.1 PNAP_RS11180 homoserine dehydrogenase
Query= SwissProt::Q5F8J4 (435 letters) >NCBI__GCF_000015505.1:WP_041376665.1 Length = 440 Score = 492 bits (1267), Expect = e-144 Identities = 257/439 (58%), Positives = 321/439 (73%), Gaps = 6/439 (1%) Query: 1 MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGREIRISAMCDLSEEKARQICPSAAFV 60 M P+ +GLLG+GTVG G VL+ N EEI RR GR I I+ + DL +A+ + V Sbjct: 1 MSPIRVGLLGIGTVGSGTFNVLKRNQEEIRRRAGRGIEITMVADLDVARAQALVGDGVLV 60 Query: 61 KDPF-ELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTANKKLLAEYGNEIFPLAE 119 D ++VA D+D+V+EL GG GIAK VL+AIE+GKH+VTANK LLA +G EIF A Sbjct: 61 VDDARKVVASPDIDIVIELIGGYGIAKALVLEAIESGKHVVTANKALLAVHGTEIFAAAH 120 Query: 120 KQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGTSNFILSEMREKGSAFADVL 179 ++ V+V FEAAVAGGIPIIKALREGL+AN I+ IAGIINGT+NFILSEMR+KG F VL Sbjct: 121 RKGVMVAFEAAVAGGIPIIKALREGLSANSIQWIAGIINGTTNFILSEMRDKGLDFGVVL 180 Query: 180 KEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMNFSACYLEGISKLDSRDIKYAE 239 KEAQ LGYAEADPTFDIEG DA HK T+MSA+AFG P+ F Y+EGI+ L ++DI+YAE Sbjct: 181 KEAQKLGYAEADPTFDIEGVDAAHKATLMSAIAFGIPVQFDKAYVEGITSLAAQDIRYAE 240 Query: 240 ELGYRIKLLGVTRKTGKGIELRVHPTLIPESRLLANVDGVMNAVRVNADMVGETLYYGAG 299 +LGYRIKLLG+T++ GIELRVHP+LIP RLLANV+G MNAV V+ D VG TLYYG G Sbjct: 241 QLGYRIKLLGITKRVAGGIELRVHPSLIPAKRLLANVEGAMNAVVVHGDAVGTTLYYGKG 300 Query: 300 AGALPTASAVVADIIDIARLVEADTAHRVPHLAFQPAQVQAQTILPMDEITSSYYLRVQA 359 AG+ PTASAV+AD++DI+RL AD HRVPHLAFQP ++ I+PM EI +SYYLR+ Sbjct: 301 AGSEPTASAVIADLVDISRLHTADPEHRVPHLAFQPDEMSDLAIVPMSEIVTSYYLRLNV 360 Query: 360 KDEPGTLGQIAALLAQENVSIEALIQKGVID-----QTTAEIVILTHSTVEKHIKSAIAA 414 DE G L + +LA+ +SI+A++Q+ D T +++ILTH VE + AIA Sbjct: 361 ADEAGVLASVTGILAEAGISIDAVLQREADDVGGEGSTQTDLIILTHDCVEAKMNEAIAQ 420 Query: 415 IEALDCVEKPITMIRMESL 433 ++AL V PIT IR E L Sbjct: 421 MQALPTVLAPITRIRKEEL 439 Lambda K H 0.318 0.135 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 525 Number of extensions: 20 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 435 Length of database: 440 Length adjustment: 32 Effective length of query: 403 Effective length of database: 408 Effective search space: 164424 Effective search space used: 164424 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory