Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate WP_011841103.1 RSPH17029_RS07920 3-deoxy-7-phosphoheptulonate synthase class II
Query= BRENDA::P29976 (525 letters) >NCBI__GCF_000015985.1:WP_011841103.1 Length = 456 Score = 542 bits (1397), Expect = e-159 Identities = 272/453 (60%), Positives = 340/453 (75%), Gaps = 4/453 (0%) Query: 75 WTPESWKLKKALQLPDYPNANELESVLKTIEAFPPIVFAGEARNLEERLADAAVGKAFLL 134 WT W+ K +Q+PDYP+A +++V + +PP+VFAGEAR L+ LA+AA G+AFLL Sbjct: 5 WTKTDWRAKPRIQMPDYPDAAAVKAVEGQLAKYPPLVFAGEARKLKAALAEAAEGRAFLL 64 Query: 135 QGGDCAESFKEFNATNIRDTFRVLLQMSIVLTFGGQVPVIKVGRMAGQFAKPRSDAFEEK 194 QGGDCAESF EF+A NIRDTFRVLLQM+IVLT+G +VPV+KVGRMAGQFAKPRS E Sbjct: 65 QGGDCAESFSEFSADNIRDTFRVLLQMAIVLTYGAKVPVVKVGRMAGQFAKPRSAPTEVI 124 Query: 195 DGVKLPSYKGDNINGDTFDEKSRIPDPNRMIRAYTQSAATLNLLRAFATGGYAAIQRVTQ 254 +G++LPSY+GD ING ++RIPDP RM++AYTQ+AA+LNLLRAF+TGG+A I RV Sbjct: 125 NGMELPSYRGDIINGFDPSPEARIPDPQRMLQAYTQAAASLNLLRAFSTGGFADIHRVHS 184 Query: 255 WNLDFVEQSEQADRYQELANRVDEALGFMSACGLG--TDHPLMTTTDFYTSHECLLLPYE 312 W L F EQ ++A+RY++++NR+ +AL FMSA G+ T H L +T DFYTSHE LLL YE Sbjct: 185 WTLGFCEQ-DKAERYRDISNRISDALDFMSAAGVNGSTSHNL-STVDFYTSHEALLLEYE 242 Query: 313 QSLTRLDSTSGLYYDCSAHMVWCGERTRQLDGAHVEFLRGIANPLGIKVSNKMDPFELVK 372 ++L R+DS +G S HM+W G+RTRQ+DGAHVEF RG+ NP+G+K +L Sbjct: 243 EALCRIDSITGQPVAGSGHMIWIGDRTRQIDGAHVEFCRGVLNPIGLKCGPSTTVEDLKV 302 Query: 373 LVEILNPNNKPGRITVIVRMGAENMRVKLPHLIRAVRRSGQIVTWVCDPMHGNTIKAPCG 432 L+ LNP N+ GR+T+I R GA + LP LIRAVR G VTW CDPMHGNTIKA G Sbjct: 303 LMARLNPQNEAGRLTLIARFGAGKVGEHLPRLIRAVREEGAKVTWCCDPMHGNTIKAASG 362 Query: 433 LKTRAFDSILAEVRAFLDVHEQEGSHAGGIHLEMTGQNVTECIGGSRTVTYDDLSSRYHT 492 KTR FDS+L EVR F +H+ EGS GG+H EMTGQ+VTEC GG R VT +DLS+RYHT Sbjct: 363 YKTRPFDSVLREVREFFSIHKAEGSIPGGVHFEMTGQDVTECTGGLRAVTDEDLSNRYHT 422 Query: 493 HCDPRLNASQSLELAFIVAERLRKRRTGSQRVS 525 CDPRLNASQSLELAF+VAE L R ++RV+ Sbjct: 423 ACDPRLNASQSLELAFLVAEELTTMREAARRVA 455 Lambda K H 0.318 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 665 Number of extensions: 15 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 525 Length of database: 456 Length adjustment: 34 Effective length of query: 491 Effective length of database: 422 Effective search space: 207202 Effective search space used: 207202 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate WP_011841103.1 RSPH17029_RS07920 (3-deoxy-7-phosphoheptulonate synthase class II)
to HMM TIGR01358 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01358.hmm # target sequence database: /tmp/gapView.2429.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01358 [M=443] Accession: TIGR01358 Description: DAHP_synth_II: 3-deoxy-7-phosphoheptulonate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.2e-214 697.9 0.0 2.5e-214 697.7 0.0 1.0 1 lcl|NCBI__GCF_000015985.1:WP_011841103.1 RSPH17029_RS07920 3-deoxy-7-phos Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000015985.1:WP_011841103.1 RSPH17029_RS07920 3-deoxy-7-phosphoheptulonate synthase class II # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 697.7 0.0 2.5e-214 2.5e-214 1 441 [. 5 446 .. 5 448 .. 0.98 Alignments for each domain: == domain 1 score: 697.7 bits; conditional E-value: 2.5e-214 TIGR01358 1 wsleswrskpaaqlPeyPdaealeavldtleslPPlvlageilklkeklaevakGeafllqgGdcaesf 69 w++ wr+kp q+P+yPda+a++av+ +l+++PPlv+age++klk+ lae+a+G+afllqgGdcaesf lcl|NCBI__GCF_000015985.1:WP_011841103.1 5 WTKTDWRAKPRIQMPDYPDAAAVKAVEGQLAKYPPLVFAGEARKLKAALAEAAEGRAFLLQGGDCAESF 73 78899**************************************************************** PP TIGR01358 70 keveadnirdklrvllqmavvltygaslPvvkvgriaGqyakPrsepieakdgvtlpsyrGdvingaaf 138 +e++adnird++rvllqma+vltyga++Pvvkvgr+aGq+akPrs+p+e+ +g++lpsyrGd+ing+++ lcl|NCBI__GCF_000015985.1:WP_011841103.1 74 SEFSADNIRDTFRVLLQMAIVLTYGAKVPVVKVGRMAGQFAKPRSAPTEVINGMELPSYRGDIINGFDP 142 ********************************************************************* PP TIGR01358 139 deaarvpdperlvrayaksaatlnllraltsgGyadlkkvheWnlefvkkspagaryeklaeeidealr 207 + +ar+pdp+r+++ay+++aa+lnllra+ +gG+ad+++vh+W+l f ++ ++++ry++++++i++al lcl|NCBI__GCF_000015985.1:WP_011841103.1 143 SPEARIPDPQRMLQAYTQAAASLNLLRAFSTGGFADIHRVHSWTLGFCEQ-DKAERYRDISNRISDALD 210 ***********************************************987.6799************** PP TIGR01358 208 fmsavgvaeae..alkrvelytsheallldyeealtrvdskegqlfdlsahllWiGertrqldgahvef 274 fmsa+gv+ ++ +l++v++ytshealll+yeeal r ds +gq s+h++WiG+rtrq+dgahvef lcl|NCBI__GCF_000015985.1:WP_011841103.1 211 FMSAAGVNGSTshNLSTVDFYTSHEALLLEYEEALCRIDSITGQPVAGSGHMIWIGDRTRQIDGAHVEF 279 ******98766569******************************************************* PP TIGR01358 275 lrgvknPigikvgpsmeadellklievldPenePGrltlisrlGaekiaeklPelleavkaaGravvWv 343 rgv nPig+k+gps+++++l l+ +l+P+ne Grltli+r+Ga k+ e+lP+l++av+++G++v+W lcl|NCBI__GCF_000015985.1:WP_011841103.1 280 CRGVLNPIGLKCGPSTTVEDLKVLMARLNPQNEAGRLTLIARFGAGKVGEHLPRLIRAVREEGAKVTWC 348 ********************************************************************* PP TIGR01358 344 tdpmhGntleaasGyktrrfddilsevkeffevhkaeGthpGGvhleltGedvteclGGareitetdla 412 +dpmhGnt++aasGyktr fd++l ev+eff +hkaeG+ pGGvh e+tG+dvtec+GG r++t++dl+ lcl|NCBI__GCF_000015985.1:WP_011841103.1 349 CDPMHGNTIKAASGYKTRPFDSVLREVREFFSIHKAEGSIPGGVHFEMTGQDVTECTGGLRAVTDEDLS 417 ********************************************************************* PP TIGR01358 413 sryetacdPrlnaeqslelaflvaeklre 441 +ry+tacdPrlna+qslelaflvae l lcl|NCBI__GCF_000015985.1:WP_011841103.1 418 NRYHTACDPRLNASQSLELAFLVAEELTT 446 *************************9876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (443 nodes) Target sequences: 1 (456 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 11.35 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory