Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate WP_011869105.1 MMARC5_RS06890 dihydroxy-acid dehydratase
Query= metacyc::MONOMER-11919 (549 letters) >NCBI__GCF_000016125.1:WP_011869105.1 Length = 550 Score = 697 bits (1800), Expect = 0.0 Identities = 347/551 (62%), Positives = 436/551 (79%), Gaps = 3/551 (0%) Query: 1 MKSDTIKRGIQRAPHRSLLARCGLTDDDFEKPFIGIANSYTDIVPGHIHLRELAEAVKEG 60 M SD +K+G+ R+P+R+LL CG +D+D EKPFIG+ NS+T++VPGHIHL+ L++AVK G Sbjct: 1 MISDNVKKGVIRSPNRALLKACGYSDEDMEKPFIGVVNSFTEVVPGHIHLKTLSDAVKHG 60 Query: 61 VNAAGGVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCD 120 V A GG FEFNT+ ICDGIAM H+GMKYSL SREI+AD VESMA AH DGLVL+PTCD Sbjct: 61 VYANGGTPFEFNTIGICDGIAMGHEGMKYSLPSREIIADAVESMARAHGFDGLVLIPTCD 120 Query: 121 KIVPGMLMAAARLDIPAIVVTGGPMLPGEFKGRKVDLINVYEGVGTVSAGEMSEDELEEL 180 KIVPGM+M A RL+IP IVVTGGPMLPGEF+G+K +LI+++EGVG G+++E+EL+ + Sbjct: 121 KIVPGMIMGALRLNIPFIVVTGGPMLPGEFQGKKCELISLFEGVGEYQVGKITEEELKSI 180 Query: 181 ERCACPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMVQ 240 E CACPG SCAGL+TAN+MACLTEALG+SLP CAT HAV ++K +IA+ +G +IV++V+ Sbjct: 181 EECACPGAGSCAGLYTANSMACLTEALGLSLPMCATIHAVDAQKVRIAKKTGSKIVDLVK 240 Query: 241 ENLKPTMIMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDELSRVIPH 300 E++KPT I+++EAFENA++VDLALGGSTNTTLHIPAIA EI+ I LD FD LS +PH Sbjct: 241 EDVKPTDILTKEAFENAILVDLALGGSTNTTLHIPAIANEIENKFITLDDFDRLSDEVPH 300 Query: 301 IASISPAGEHMMLDLDRAGGIPAVLKTLEDHINRECVTCTGRTVQENIENVKVGHRDVIR 360 IASI P GEH M+DL AGGIPAVLK L++ I R T GR+ E E+VK + DVIR Sbjct: 301 IASIKPGGEHYMIDLHNAGGIPAVLKVLKEKI-RNTKTVDGRSTLEIAESVKYVNYDVIR 359 Query: 361 PLDSPVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSEDECMEAIFGGRI 420 +++PVH GL +L+GNLAP G VVK GAV M HEGPAKV+NSEDE + AI GG+I Sbjct: 360 KVEAPVHETAGLRVLKGNLAPNGCVVKIGAVDPKMHKHEGPAKVYNSEDEAIAAILGGKI 419 Query: 421 DEGDVIVIRYEGPKGGPGMREMLNPTSAIAGMGL-ERVALITDGRFSGGTRGPCVGHVSP 479 EGDV+VIR+EGP GGPGMREML+PTSAI GMGL + VALITDGRFSGG+RGPC+GHVSP Sbjct: 420 VEGDVVVIRHEGPSGGPGMREMLSPTSAICGMGLDDSVALITDGRFSGGSRGPCIGHVSP 479 Query: 480 EAMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREIEERLQSAVKPRRSV-KGWLARYRKL 538 EA G +AA+ +GDII+ID+ +++ VDL I+ERL + + KG+L+RY +L Sbjct: 480 EAAAGGLIAAIENGDIIKIDMIEKEINVDLDESVIKERLSKLEEFEPKIKKGYLSRYSRL 539 Query: 539 AGSADTGAVLR 549 SAD GAVL+ Sbjct: 540 VSSADEGAVLK 550 Lambda K H 0.319 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1036 Number of extensions: 52 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 549 Length of database: 550 Length adjustment: 36 Effective length of query: 513 Effective length of database: 514 Effective search space: 263682 Effective search space used: 263682 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate WP_011869105.1 MMARC5_RS06890 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.32158.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.3e-258 842.4 6.7 8.3e-258 842.2 6.7 1.0 1 lcl|NCBI__GCF_000016125.1:WP_011869105.1 MMARC5_RS06890 dihydroxy-acid de Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000016125.1:WP_011869105.1 MMARC5_RS06890 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 842.2 6.7 8.3e-258 8.3e-258 1 542 [. 14 550 .] 14 550 .] 0.99 Alignments for each domain: == domain 1 score: 842.2 bits; conditional E-value: 8.3e-258 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 ++rallka+G++ded+ekP+i+vvns+te+vPgh+hlk l+++vk++++a+Gg+++efnti+++DGiam lcl|NCBI__GCF_000016125.1:WP_011869105.1 14 PNRALLKACGYSDEDMEKPFIGVVNSFTEVVPGHIHLKTLSDAVKHGVYANGGTPFEFNTIGICDGIAM 82 68******************************************************************* PP TIGR00110 70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138 gheGmkysLpsreiiaD+ve++++ah +D+lv+i++CDkivPGm+m+alrlniP+ivv+GGpm +g+++ lcl|NCBI__GCF_000016125.1:WP_011869105.1 83 GHEGMKYSLPSREIIADAVESMARAHGFDGLVLIPTCDKIVPGMIMGALRLNIPFIVVTGGPMLPGEFQ 151 ********************************************************************* PP TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207 ++k +l+++fe+vgey+ gk++eeel++ie++acP+agsC+Gl+tansmacltealGlslP ++t++a lcl|NCBI__GCF_000016125.1:WP_011869105.1 152 -GKKCELISLFEGVGEYQVGKITEEELKSIEECACPGAGSCAGLYTANSMACLTEALGLSLPMCATIHA 219 .9******************************************************************* PP TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeag.vklsl 275 ++a+k ++akk+g +iv+lvk+++kP+diltkeafenai +dlalGGstnt+Lh++aia+e++ ++l lcl|NCBI__GCF_000016125.1:WP_011869105.1 220 VDAQKVRIAKKTGSKIVDLVKEDVKPTDILTKEAFENAILVDLALGGSTNTTLHIPAIANEIEnKFITL 288 **************************************************************96778** PP TIGR00110 276 ddfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlr 344 ddfdrls++vP++a++kP+g+++++dlh+aGG++avlk+l ++++ ++++tv G++ e++e+vk+ lcl|NCBI__GCF_000016125.1:WP_011869105.1 289 DDFDRLSDEVPHIASIKPGGEHYMIDLHNAGGIPAVLKVL--KEKI-RNTKTVDGRSTLEIAESVKYV- 353 ****************************************..6666.578****************99. PP TIGR00110 345 vdqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvke 413 ++dvir+++ pv++++gl+vLkGnla++G+vvki++v+ ++ k+eGpakv++se+ea++ailggk+ e lcl|NCBI__GCF_000016125.1:WP_011869105.1 354 -NYDVIRKVEAPVHETAGLRVLKGNLAPNGCVVKIGAVDPKMHKHEGPAKVYNSEDEAIAAILGGKIVE 421 .******************************************************************** PP TIGR00110 414 GdvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialv 482 Gdvvvir+eGP GgPGmremL Ptsa++g+GL+++vaLitDGrfsGg+rG++iGhvsPeaa+gG ia + lcl|NCBI__GCF_000016125.1:WP_011869105.1 422 GDVVVIRHEGPSGGPGMREMLSPTSAICGMGLDDSVALITDGRFSGGSRGPCIGHVSPEAAAGGLIAAI 490 ********************************************************************* PP TIGR00110 483 edGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavld 542 e+GD+ikiD+ +++++++++e ++er k ++ e++ +kg+L++y++lvssad+Gavl+ lcl|NCBI__GCF_000016125.1:WP_011869105.1 491 ENGDIIKIDMIEKEINVDLDESVIKERLSKLEEFEPKIKKGYLSRYSRLVSSADEGAVLK 550 **********************************************************85 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (550 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 10.29 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory