Align phosphoserine aminotransferase monomer (EC 2.6.1.52; EC 2.6.1.1) (characterized)
to candidate WP_012566971.1 RC1_RS08575 phosphoserine transaminase
Query= metacyc::MONOMER-15918 (370 letters) >NCBI__GCF_000016185.1:WP_012566971.1 Length = 386 Score = 477 bits (1227), Expect = e-139 Identities = 228/379 (60%), Positives = 280/379 (73%), Gaps = 10/379 (2%) Query: 2 KPTRVPKNPCFSSGPCAKHPGYSVEELKDTPFGRSHRSKPGKEKLAEAIKRTRDMLGLPD 61 KP P NP FSSGPCAK PG+++E LK GRSHRSKPGK KL + I ++++LG+P Sbjct: 6 KPDSRPANPQFSSGPCAKRPGWTIEALKGALVGRSHRSKPGKAKLEQVITLSKEILGIPA 65 Query: 62 DYFVGIVPASDTGAFEMCLWSMLGCRGVDVLVWESFSKGWATDITKQLKLKDTRVFEAEY 121 Y +GIVPASDTGA EM LWS+LG RG+D+L WESF K W TD+ KQL+L D R F+A Y Sbjct: 66 GYRLGIVPASDTGAVEMALWSLLGARGIDMLAWESFGKDWVTDVVKQLRLTDVREFKAPY 125 Query: 122 GKLPDLKKVDFKNDVVFVWNGTTSGVKVPNADWIPDDREGVTLCDATSAIFAMDIPYHKL 181 G LPDL +VDF DVVF WNGTTSGV+VP+ DWIPDDR+G+T+CDATSA FAMD+P+ KL Sbjct: 126 GALPDLTQVDFARDVVFTWNGTTSGVRVPDGDWIPDDRQGLTICDATSATFAMDLPWSKL 185 Query: 182 DVITFSWQKVLGGEGAHGMLILSPRAVQRLESYTPAWPLPKIFRLTKGGKLNKDIFAGST 241 DV+T+SWQKVLGGE AHGML+LSPRAV+RLES+ PAWP+PKIFR+TK GK+ + IF G T Sbjct: 186 DVVTWSWQKVLGGEAAHGMLVLSPRAVERLESHKPAWPMPKIFRMTKDGKIVEGIFKGET 245 Query: 242 INTPSMLANEDWLATLKWAESVGGLKQLIRRTNENLAVFEAFVAKNNWIHFLAETKEIRS 301 INTPSM+A ED L L WA+S+GGL LI R+ NL +VA+ +W+ FL + E RS Sbjct: 246 INTPSMIAVEDALDGLTWAKSIGGLPALIGRSEANLKAVTDWVARTDWVDFLPQRPETRS 305 Query: 302 STSVCFKV----------DLSDEKLKELIKTLEKEKVAYDIGSYRDAPSGLRIWCGATVE 351 TS+C K+ + E K++ LEKE VA D G+YRDAP GLRIW GATVE Sbjct: 306 CTSICLKIVDPWATGQSAEAQAEIAKKVAALLEKEGVALDAGAYRDAPPGLRIWGGATVE 365 Query: 352 KEDLECLCEWIEWAYNLVK 370 D++ L W++WAY VK Sbjct: 366 TADIQALLPWLDWAYATVK 384 Lambda K H 0.319 0.136 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 535 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 370 Length of database: 386 Length adjustment: 30 Effective length of query: 340 Effective length of database: 356 Effective search space: 121040 Effective search space used: 121040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_012566971.1 RC1_RS08575 (phosphoserine transaminase)
to HMM TIGR01365 (phosphoserine aminotransferase (EC 2.6.1.52))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01365.hmm # target sequence database: /tmp/gapView.14091.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01365 [M=374] Accession: TIGR01365 Description: serC_2: phosphoserine aminotransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.1e-202 658.9 1.6 1.3e-202 658.7 1.6 1.0 1 lcl|NCBI__GCF_000016185.1:WP_012566971.1 RC1_RS08575 phosphoserine transa Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000016185.1:WP_012566971.1 RC1_RS08575 phosphoserine transaminase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 658.7 1.6 1.3e-202 1.3e-202 1 374 [] 10 383 .. 10 383 .. 1.00 Alignments for each domain: == domain 1 score: 658.7 bits; conditional E-value: 1.3e-202 TIGR01365 1 rpanpefssgpcakrpgysveelknaalgrshrsklgkeklkeaiektrevlevpadyligivaasdtg 69 rpanp+fssgpcakrpg+++e+lk a +grshrsk gk+kl++ i ++e+l++pa y++giv+asdtg lcl|NCBI__GCF_000016185.1:WP_012566971.1 10 RPANPQFSSGPCAKRPGWTIEALKGALVGRSHRSKPGKAKLEQVITLSKEILGIPAGYRLGIVPASDTG 78 7******************************************************************** PP TIGR01365 70 avemalwsllgargvdllafesfgkgwvtdvtkqlklkdvrvleaeygklpdlkkvdfkkdvvftwngt 138 avemalwsllgarg+d+la+esfgk+wvtdv+kql+l dvr ++a+yg lpdl++vdf++dvvftwngt lcl|NCBI__GCF_000016185.1:WP_012566971.1 79 AVEMALWSLLGARGIDMLAWESFGKDWVTDVVKQLRLTDVREFKAPYGALPDLTQVDFARDVVFTWNGT 147 ********************************************************************* PP TIGR01365 139 tsgvrvpngdfipadreglticdatsaafaqdldyekldvvtfswqkvlggegahgvlilspravarle 207 tsgvrvp+gd+ip+dr+glticdatsa fa+dl+++kldvvt+swqkvlgge ahg+l+lsprav+rle lcl|NCBI__GCF_000016185.1:WP_012566971.1 148 TSGVRVPDGDWIPDDRQGLTICDATSATFAMDLPWSKLDVVTWSWQKVLGGEAAHGMLVLSPRAVERLE 216 ********************************************************************* PP TIGR01365 208 sytpawplpkifrltkggklskdifegetintpsmlavedaldalkwaesigglkalvaraddnlavle 276 s++pawp+pkifr+tk gk+ ++if+getintpsm+avedald l wa+siggl al+ r+++nl+ + lcl|NCBI__GCF_000016185.1:WP_012566971.1 217 SHKPAWPMPKIFRMTKDGKIVEGIFKGETINTPSMIAVEDALDGLTWAKSIGGLPALIGRSEANLKAVT 285 ********************************************************************* PP TIGR01365 277 afvaksswvdflaatkeirsntsvclkvvdpdvaaldedaqadfakelvsalekegvaydigsyrdapa 345 ++va+++wvdfl +++e+rs ts+clk+vdp + ++aqa++ak+++ +lekegva+d g+yrdap+ lcl|NCBI__GCF_000016185.1:WP_012566971.1 286 DWVARTDWVDFLPQRPETRSCTSICLKIVDPWATGQSAEAQAEIAKKVAALLEKEGVALDAGAYRDAPP 354 ********************************************************************* PP TIGR01365 346 glriwcgatveksdleallewldwafalv 374 glriw gatve+ d++all+wldwa+a+v lcl|NCBI__GCF_000016185.1:WP_012566971.1 355 GLRIWGGATVETADIQALLPWLDWAYATV 383 **************************986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (374 nodes) Target sequences: 1 (386 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.01 # Mc/sec: 12.61 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory