Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized)
to candidate WP_011881174.1 dihydroxy-acid dehydratase
Query= curated2:A8AB39 (552 letters) >NCBI__GCF_000016205.1:WP_011881174.1 Length = 583 Score = 367 bits (943), Expect = e-106 Identities = 218/553 (39%), Positives = 325/553 (58%), Gaps = 22/553 (3%) Query: 15 HRALWRASGLIDEELR-RPLIGVANSWNEIVPGHVHLDKVAEAVKAGIRMAGGTPLEFGT 73 +R+ + G+ D E RP+IG+ N+W+E+ P + H K+AE VK G+ AGG P+EF Sbjct: 24 YRSWMKNQGIPDHEFDGRPIIGICNTWSELTPCNAHFRKLAEHVKRGVFEAGGFPVEFPV 83 Query: 74 IAVCDGIAMGHEGMRYS-LPSREVIADTVEIMVEAHRLDAVVMVTNCDKITPGFLLAAAR 132 + G +R S + +R + + VE + + +DAVV++ CDK TP L+ AA Sbjct: 84 FS------NGESNLRPSAMLTRNLASMDVEEAIRGNPIDAVVLLAGCDKTTPALLMGAAS 137 Query: 133 LEVPVILINGGPMMPGVYGKERIDFKDLMERMNVLIKEGRTE--ELRKLEESALPGPGSC 190 +VP I+++GGPM+ G + I + +++ +K G + E G+C Sbjct: 138 CDVPAIVVSGGPMLNGKLEGKNIGSGTAVWQLHEALKAGEIDLHHFLSAEAGMSRSAGTC 197 Query: 191 AGLFTANTMNMLSEAMGLMLPGASTVPAVEARRLWYAKLTGMRIVKMVEEGLTPDKILTR 250 + TA+TM ++EA+G+ LP + +PAV++RR A ++G+RIV+M EGL K+LTR Sbjct: 198 NTMGTASTMACMAEALGVALPHNAAIPAVDSRRYVLAHMSGIRIVEMALEGLVLSKVLTR 257 Query: 251 KALENAIAVDMALGGSTNSVLHLEALAYELGIDLPLEVFDEISRKVPHIASISPSGRHFV 310 A ENAI + A+GGSTN+V+HL+A+A +G+ L LE + I R P I + PSGR + Sbjct: 258 AAFENAIRANAAIGGSTNAVIHLKAIAGRIGVPLELEDWMRIGRDTPTIVDLMPSGRFLM 317 Query: 311 VDLDRAGGIPAVLKELGEAGLI-HKDALTVTGKTVWENVKDAAVLDREVIRPLDNPYSPF 369 + AGG+PAVL+ LGE GL+ H DALTV GK++W+NV++A D EVIRPLD P Sbjct: 318 EEFYYAGGLPAVLRRLGEGGLLPHPDALTVNGKSLWDNVREAPNYDDEVIRPLDRPLIAD 377 Query: 370 GGLAILKGSLAPNGAVVKASAVKRELWKFKGVARVFDREEDAVKAIRGG--EIEPGTVIV 427 GG+ IL+G+LAP GAV+K SA EL K +G A VF+ E I +++ +V+V Sbjct: 378 GGICILRGNLAPRGAVLKPSAASPELLKHRGRAVVFENLEHYKATINDESLDVDASSVMV 437 Query: 428 IRYEGPRGGPGMREM--LTATAAVMALGLGDKVALVTDGRFSGATRGPAIGHVSPEAAAG 485 ++ GPRG PGM E+ + ++ G+ D V ++D R SG G + HV+PEAAAG Sbjct: 438 LKNCGPRGYPGMAEVGNMGLPPKLLRQGVKDMVR-ISDARMSGTAYGTVVLHVAPEAAAG 496 Query: 486 GPIALVQDGDEIVIDIEKRRLDLLVDEKELEERRARWKPKVKP------LRRGILRRYAK 539 GP+A V++GD I +D E L L + + EL+ R + P P + G R Y Sbjct: 497 GPLAAVRNGDWIELDCEAGTLHLDITDDELQRRLSDVDPTAAPGVSAQLGKGGYARLYID 556 Query: 540 MALSADKGGALEY 552 L AD+G L++ Sbjct: 557 HVLQADEGCDLDF 569 Lambda K H 0.319 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 881 Number of extensions: 41 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 552 Length of database: 583 Length adjustment: 36 Effective length of query: 516 Effective length of database: 547 Effective search space: 282252 Effective search space used: 282252 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory