Alignments for a candidate for aroG in Sphingomonas wittichii RW1

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroG in Sphingomonas wittichii RW1

Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate WP_011951238.1 SWIT_RS01945 3-deoxy-7-phosphoheptulonate synthase class II

Query= BRENDA::P29976
         (525 letters)



>NCBI__GCF_000016765.1:WP_011951238.1
          Length = 458

 Score =  550 bits (1416), Expect = e-161
 Identities = 270/456 (59%), Positives = 337/456 (73%), Gaps = 2/456 (0%)

Query: 72  ALKWTPESWKLKKALQLPDYPNANELESVLKTIEAFPPIVFAGEARNLEERLADAAVGKA 131
           A  WTP SW+ ++A+Q+PDYP+A  L +  + + +FPP+VFAGEAR L   LA  A GKA
Sbjct: 2   ATNWTPASWRQQQAVQMPDYPDAAALAAAEQELSSFPPLVFAGEARELTASLAQVAAGKA 61

Query: 132 FLLQGGDCAESFKEFNATNIRDTFRVLLQMSIVLTFGGQVPVIKVGRMAGQFAKPRSDAF 191
           FLLQGGDCAESF EF+  NIRDTFRVLLQM++VLTF  ++PV+KVGRMAGQFAKPRS   
Sbjct: 62  FLLQGGDCAESFAEFHPNNIRDTFRVLLQMAVVLTFASKLPVVKVGRMAGQFAKPRSAPT 121

Query: 192 EEKDGVKLPSYKGDNINGDTFDEKSRIPDPNRMIRAYTQSAATLNLLRAFATGGYAAIQR 251
           EE  GV LPSY+GD IN   F E+SRIPDP RM+RAY+QSAATLNLLRAFA GGYA + +
Sbjct: 122 EEIGGVTLPSYRGDIINDIGFSEESRIPDPRRMVRAYSQSAATLNLLRAFAQGGYANLHQ 181

Query: 252 VTQWNLDFVEQSEQADRYQELANRVDEALGFMSACGLGTDH-PLMTTTDFYTSHECLLLP 310
           V  W LDF+ +S  A +Y E+A R+ +AL FM+ACG+  +  P +  T FYTSHE LLLP
Sbjct: 182 VHAWTLDFMGRSPWAAQYGEVAARIGDALDFMAACGINPETVPQLKGTQFYTSHEALLLP 241

Query: 311 YEQSLTRLDS-TSGLYYDCSAHMVWCGERTRQLDGAHVEFLRGIANPLGIKVSNKMDPFE 369
           YEQ++TR DS  +G Y+D SAH +W G+RTR    AHVE+LRG+ NP+G+K    ++P  
Sbjct: 242 YEQAMTREDSLNTGQYFDTSAHFLWIGDRTRFEGSAHVEYLRGVGNPIGLKCGPSLEPDA 301

Query: 370 LVKLVEILNPNNKPGRITVIVRMGAENMRVKLPHLIRAVRRSGQIVTWVCDPMHGNTIKA 429
           L++L++ L+P  +PGRIT+I R G + +   LP L+RAV+R G+ V W CDPMHGN +KA
Sbjct: 302 LLRLLDTLDPQRQPGRITLITRYGHDKIEKHLPALVRAVKREGRPVVWSCDPMHGNVVKA 361

Query: 430 PCGLKTRAFDSILAEVRAFLDVHEQEGSHAGGIHLEMTGQNVTECIGGSRTVTYDDLSSR 489
             G KTR FD ILAEVR F  VH  EG+H GGIH EMTGQNVTEC GG+  VT ++L+ R
Sbjct: 362 ANGYKTRPFDRILAEVRGFFAVHRAEGTHGGGIHAEMTGQNVTECTGGAMAVTDENLADR 421

Query: 490 YHTHCDPRLNASQSLELAFIVAERLRKRRTGSQRVS 525
           YHTHCDPRLNA QSLELAF++AE L +      R +
Sbjct: 422 YHTHCDPRLNAGQSLELAFLLAEMLNQEMKERDRAA 457


Lambda     K      H
   0.318    0.132    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 657
Number of extensions: 19
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 525
Length of database: 458
Length adjustment: 34
Effective length of query: 491
Effective length of database: 424
Effective search space:   208184
Effective search space used:   208184
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

Align candidate WP_011951238.1 SWIT_RS01945 (3-deoxy-7-phosphoheptulonate synthase class II)
to HMM TIGR01358 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01358.hmm
# target sequence database:        /tmp/gapView.14889.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01358  [M=443]
Accession:   TIGR01358
Description: DAHP_synth_II: 3-deoxy-7-phosphoheptulonate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   4.3e-219  713.4   0.0   5.1e-219  713.2   0.0    1.0  1  lcl|NCBI__GCF_000016765.1:WP_011951238.1  SWIT_RS01945 3-deoxy-7-phosphohe


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000016765.1:WP_011951238.1  SWIT_RS01945 3-deoxy-7-phosphoheptulonate synthase class II
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  713.2   0.0  5.1e-219  5.1e-219       1     441 [.       5     448 ..       5     450 .. 0.99

  Alignments for each domain:
  == domain 1  score: 713.2 bits;  conditional E-value: 5.1e-219
                                 TIGR01358   1 wsleswrskpaaqlPeyPdaealeavldtleslPPlvlageilklkeklaevakGeafllqgGdcaesf 69 
                                               w+++swr+++a+q+P+yPda+al a +++l+s+PPlv+age+++l ++la+va+G+afllqgGdcaesf
  lcl|NCBI__GCF_000016765.1:WP_011951238.1   5 WTPASWRQQQAVQMPDYPDAAALAAAEQELSSFPPLVFAGEARELTASLAQVAAGKAFLLQGGDCAESF 73 
                                               89******************************************************************* PP

                                 TIGR01358  70 keveadnirdklrvllqmavvltygaslPvvkvgriaGqyakPrsepieakdgvtlpsyrGdvingaaf 138
                                                e++ +nird++rvllqmavvlt++++lPvvkvgr+aGq+akPrs+p+e+ +gvtlpsyrGd+in   f
  lcl|NCBI__GCF_000016765.1:WP_011951238.1  74 AEFHPNNIRDTFRVLLQMAVVLTFASKLPVVKVGRMAGQFAKPRSAPTEEIGGVTLPSYRGDIINDIGF 142
                                               ********************************************************************* PP

                                 TIGR01358 139 deaarvpdperlvrayaksaatlnllraltsgGyadlkkvheWnlefvkkspagaryeklaeeidealr 207
                                               +e++r+pdp+r+vray++saatlnllra+++gGya+l++vh+W+l+f+++sp +a+y ++a +i++al 
  lcl|NCBI__GCF_000016765.1:WP_011951238.1 143 SEESRIPDPRRMVRAYSQSAATLNLLRAFAQGGYANLHQVHAWTLDFMGRSPWAAQYGEVAARIGDALD 211
                                               ********************************************************************* PP

                                 TIGR01358 208 fmsavgvaeae..alkrvelytsheallldyeealtrvds.kegqlfdlsahllWiGertrqldgahve 273
                                               fm a+g+++++  +lk +++ytshealll+ye+a+tr+ds ++gq+fd+sah+lWiG+rtr    ahve
  lcl|NCBI__GCF_000016765.1:WP_011951238.1 212 FMAACGINPETvpQLKGTQFYTSHEALLLPYEQAMTREDSlNTGQYFDTSAHFLWIGDRTRFEGSAHVE 280
                                               ********999999*************************97899************************* PP

                                 TIGR01358 274 flrgvknPigikvgpsmeadellklievldPenePGrltlisrlGaekiaeklPelleavkaaGravvW 342
                                               +lrgv nPig+k+gps+e+d ll+l++ ldP+++PGr+tli+r G +ki ++lP l++avk++Gr vvW
  lcl|NCBI__GCF_000016765.1:WP_011951238.1 281 YLRGVGNPIGLKCGPSLEPDALLRLLDTLDPQRQPGRITLITRYGHDKIEKHLPALVRAVKREGRPVVW 349
                                               ********************************************************************* PP

                                 TIGR01358 343 vtdpmhGntleaasGyktrrfddilsevkeffevhkaeGthpGGvhleltGedvteclGGareitetdl 411
                                                +dpmhGn ++aa+Gyktr fd+il+ev++ff+vh+aeGth GG+h e+tG++vtec+GGa ++t+++l
  lcl|NCBI__GCF_000016765.1:WP_011951238.1 350 SCDPMHGNVVKAANGYKTRPFDRILAEVRGFFAVHRAEGTHGGGIHAEMTGQNVTECTGGAMAVTDENL 418
                                               ********************************************************************* PP

                                 TIGR01358 412 asryetacdPrlnaeqslelaflvaeklre 441
                                               a+ry+t+cdPrlna qslelafl+ae+l++
  lcl|NCBI__GCF_000016765.1:WP_011951238.1 419 ADRYHTHCDPRLNAGQSLELAFLLAEMLNQ 448
                                               ***************************987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (443 nodes)
Target sequences:                          1  (458 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 12.33
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis