Alignments for a candidate for metC in Sphingomonas wittichii RW1

GapMind for Amino acid biosynthesis

Alignments for a candidate for metC in Sphingomonas wittichii RW1

Align alanine racemase (EC 5.1.1.1) (characterized)
to candidate WP_012049960.1 SWIT_RS19075 cystathionine beta-lyase

Query= BRENDA::P06721
         (395 letters)



>NCBI__GCF_000016765.1:WP_012049960.1
          Length = 398

 Score =  362 bits (929), Expect = e-104
 Identities = 197/390 (50%), Positives = 255/390 (65%), Gaps = 8/390 (2%)

Query: 5   KLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHATRNRANGELFYGRRGTLTH 64
           K  T+LV AGR  K   G VN  + RAS+++FDSV   +   R   +G L+YGRRGT T 
Sbjct: 10  KPGTRLVTAGRHAKNRRGIVNPPVWRASTILFDSVAELRAGVRKPDDG-LYYGRRGTPTQ 68

Query: 65  FSLQQAMCELE-GGAGCVLFPCGAAAVANSILAFIEQGDHVLMTNTAYEPSQDFCSKILS 123
           +SL +A+ ELE G AG VL+P G AA++  +L+ +  GD +LM ++AYEPS+  C+ +L 
Sbjct: 69  WSLAEALTELEPGAAGTVLYPSGVAAISAVLLSLLAPGDELLMVDSAYEPSRAICNGLLR 128

Query: 124 KLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRSVVPDAIIM 183
           + G+ T ++DP IGA I   +   T++VFLESPGS+T EV DVPAI AA R+       +
Sbjct: 129 RFGIETRFYDPAIGAGIADLIGDRTRVVFLESPGSLTFEVQDVPAIAAAARA--RGVTTV 186

Query: 184 IDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLMGQM 243
           +DNTWA  +LF AL  G+D+SI A TKY+VGHSD M+G+A      +E+LR  ++ +GQ 
Sbjct: 187 LDNTWATPLLFPALAHGVDISILACTKYIVGHSDVMMGSATAVPGHFEKLRRTSHALGQC 246

Query: 244 VDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWKRD 303
           V  D AY+ +RGLRTL +RLRQH E +L VA WLAE PQVARV HPALP   GHE WKRD
Sbjct: 247 VSPDDAYLAARGLRTLQLRLRQHEEGALAVARWLAEQPQVARVLHPALPDCPGHELWKRD 306

Query: 304 FTGSSGLFSFVLKKKLNNEELANYLDNFSLFSMAYSWGGYESLILANQPEHIAAIRPQGE 363
           F+G+SGLFS VL    ++   A  +D   LF + +SWGGYESL L   P   A +R    
Sbjct: 307 FSGASGLFSIVLDGG-DDAARAALIDGLDLFGIGFSWGGYESLALPVDP---AGLRTATS 362

Query: 364 IDFSGTLIRLHIGLEDVDDLIADLDAGFAR 393
               G  IRLHIG+ED  DLIADL  G AR
Sbjct: 363 WQAEGPAIRLHIGIEDPADLIADLGRGLAR 392


Lambda     K      H
   0.321    0.135    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 455
Number of extensions: 21
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 395
Length of database: 398
Length adjustment: 31
Effective length of query: 364
Effective length of database: 367
Effective search space:   133588
Effective search space used:   133588
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis