Align homoserine kinase (EC 2.7.1.39) (characterized)
to candidate WP_012048899.1 SWIT_RS13620 homoserine kinase
Query= reanno::Korea:Ga0059261_3253 (319 letters) >NCBI__GCF_000016765.1:WP_012048899.1 Length = 320 Score = 387 bits (995), Expect = e-112 Identities = 192/311 (61%), Positives = 231/311 (74%), Gaps = 1/311 (0%) Query: 1 MAVYTQVSAEALAGFLARYDVGELVSAKGIAEGVENSNYLVETTRDRFILTLYEKRVEAA 60 MAVYTQVS E + FL RYDVG L+SAKGIAEGVENSNYL+ETT RFILTLYEKRV Sbjct: 1 MAVYTQVSTEEMTAFLHRYDVGTLLSAKGIAEGVENSNYLIETTAGRFILTLYEKRVNVD 60 Query: 61 DLPYFMGLLDHLAAKGLPVPPAIKDRGGVEIQELNGRPACLIKFLSGISLSHPTPAQARA 120 DLP+F L DHLAAKGL +P ++DR G ++Q L GRPACLI+FLSG+S++HPTPAQARA Sbjct: 61 DLPFFCALTDHLAAKGLNIPRMLRDRRGEQLQTLAGRPACLIEFLSGVSVTHPTPAQARA 120 Query: 121 AGEAMAQMHRAVADFPLDRPNSMGVDTWQPLFEKCGHSLDQIVPGLYDDLGFAIARVVPA 180 AG A+ MH+A+ DF L RPNS+ + W L +CG LD I PGL + + + Sbjct: 121 AGGALGDMHKALGDFRLGRPNSLDLAGWGALAARCGADLDHIQPGLRGRVMAEVEWLEAN 180 Query: 181 WTRNDFDRCAIHADLFPDNVLMRGDQVTGLIDFYFACTDIRVYDLAVMHSAWSFDAHGRN 240 W R+ IHADLFPDNVLM GD+V+G+IDFYFACTDIR +D+AV H+AW F+ G Sbjct: 181 WPRH-LRTAVIHADLFPDNVLMLGDEVSGVIDFYFACTDIRAWDVAVTHAAWCFENDGTG 239 Query: 241 YDPAVGDALIAGYEASFPLTEVERAAFPTLAAGACIRFSLSRAWDWLNTPADALVMRKDP 300 Y +G AL+AGY+ F L+ ER AFP LA GAC+RF L+RAWDWLNTPADALV RKDP Sbjct: 240 YHADLGRALVAGYDRQFGLSTDERLAFPILARGACLRFLLTRAWDWLNTPADALVTRKDP 299 Query: 301 LAYVRRLKHYA 311 +A++RRL YA Sbjct: 300 IAFLRRLDFYA 310 Lambda K H 0.323 0.138 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 341 Number of extensions: 11 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 319 Length of database: 320 Length adjustment: 28 Effective length of query: 291 Effective length of database: 292 Effective search space: 84972 Effective search space used: 84972 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 48 (23.1 bits)
Align candidate WP_012048899.1 SWIT_RS13620 (homoserine kinase)
to HMM TIGR00938 (thrB: homoserine kinase (EC 2.7.1.39))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00938.hmm # target sequence database: /tmp/gapView.5705.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00938 [M=307] Accession: TIGR00938 Description: thrB_alt: homoserine kinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.3e-123 395.9 0.0 7.2e-123 395.7 0.0 1.0 1 lcl|NCBI__GCF_000016765.1:WP_012048899.1 SWIT_RS13620 homoserine kinase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000016765.1:WP_012048899.1 SWIT_RS13620 homoserine kinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 395.7 0.0 7.2e-123 7.2e-123 1 306 [. 1 307 [. 1 308 [. 0.99 Alignments for each domain: == domain 1 score: 395.7 bits; conditional E-value: 7.2e-123 TIGR00938 1 mavytsvsdeeleafLegydlGellslkGiaeGvensnyllttdkgryvLtlyekrvkaeeLPfflell 69 mavyt+vs ee+ afL+ yd+G lls kGiaeGvensnyl++t+ gr++Ltlyekrv+ ++LPff +l+ lcl|NCBI__GCF_000016765.1:WP_012048899.1 1 MAVYTQVSTEEMTAFLHRYDVGTLLSAKGIAEGVENSNYLIETTAGRFILTLYEKRVNVDDLPFFCALT 69 9******************************************************************** PP TIGR00938 70 thLaerglpvakpvksrdGralseLaGkPaalvefLkGssvakPtaercrevgevlaklhlagadfkee 138 +hLa++gl +++ ++ r G++l++LaG+Pa+l+efL+G sv++Pt++++r++g l +h a df+ + lcl|NCBI__GCF_000016765.1:WP_012048899.1 70 DHLAAKGLNIPRMLRDRRGEQLQTLAGRPACLIEFLSGVSVTHPTPAQARAAGGALGDMHKALGDFRLG 138 ********************************************************************* PP TIGR00938 139 rkndlrleaWsilaakkfkvleqleeelaalldkeldalkkflprdLPrgvihadlfkdnvlldgdklk 207 r n+l+l+ W laa++ l++++++l + +e++ l+ ++pr+L ++vihadlf+dnvl+ gd+++ lcl|NCBI__GCF_000016765.1:WP_012048899.1 139 RPNSLDLAGWGALAARCGADLDHIQPGLRGRVMAEVEWLEANWPRHLRTAVIHADLFPDNVLMLGDEVS 207 *****************999************************************************* PP TIGR00938 208 gvidfyfaCedallydlaiavndWcfeaddk.ldaaaakallkgyeavrpLseeekaafpvllrgaalr 275 gvidfyfaC+d +++d+a++ +Wcfe+d++ ++a+ +al+ gy++++ Ls+ e+ afp+l+rga+lr lcl|NCBI__GCF_000016765.1:WP_012048899.1 208 GVIDFYFACTDIRAWDVAVTHAAWCFENDGTgYHADLGRALVAGYDRQFGLSTDERLAFPILARGACLR 276 *****************************875999********************************** PP TIGR00938 276 fllsrlldlvftqagelvvakdPaeferkLk 306 fll+r++d+ +t+a++lv+ kdP +f r+L lcl|NCBI__GCF_000016765.1:WP_012048899.1 277 FLLTRAWDWLNTPADALVTRKDPIAFLRRLD 307 ****************************996 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (307 nodes) Target sequences: 1 (320 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 9.44 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory