Alignments for a candidate for ilvD in Sphingomonas wittichii RW1

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Sphingomonas wittichii RW1

Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (characterized)
to candidate WP_086014462.1 SWIT_RS08390 dihydroxy-acid dehydratase

Query= SwissProt::P9WKJ5
         (575 letters)



>NCBI__GCF_000016765.1:WP_086014462.1
          Length = 571

 Score =  483 bits (1243), Expect = e-141
 Identities = 258/559 (46%), Positives = 353/559 (63%), Gaps = 10/559 (1%)

Query: 18  KPRSRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNEITPCNLSLDRLANAVKE 77
           K RS  + +G+ +   R  L A+G DDED  +P + V  +  E++PCN +L   A   K 
Sbjct: 8   KFRSEIIREGVVRTTTRSFLYALGEDDEDLRQPHVAVIHTGGEMSPCNTTLRDQALHAKT 67

Query: 78  GVFSAGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVEVVMQAERLDGSVLLAGC 137
           G+++ GG   E   +SVSDG+S+ H GM FSL+SRE+IADSVE  +++ + DG + + GC
Sbjct: 68  GIYAGGGTAHECPVVSVSDGLSVAHSGMRFSLISRELIADSVEATVRSHQWDGVLAVGGC 127

Query: 138 DKSLPGMLMAAARLDLAAVFLYAGSILPGRAKLSDGSERDVTIIDAFEAVGACSRGLMSR 197
           DK++PG++M   R ++ +VFLY G+ LPG         RDV I+D +E +G    G  ++
Sbjct: 128 DKNMPGLMMGIVRCNVPSVFLYGGAALPGHL-----GGRDVNIVDTYEMIGRVLAGEATQ 182

Query: 198 ADVDAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAPPATDRRRDGFARRSGQA 257
            +++ + RA  P  GAC G YTANTM   +EALG++L GS+  PA    R   AR++G+ 
Sbjct: 183 GELEDMARACLPTAGACAGQYTANTMGMVSEALGLALLGSSMIPAVYAARAPMARKAGKV 242

Query: 258 VVE-LLRRGITARDILTKEAFENAIAVVMAFGGSTNAVLHLLAIAHEANVALSLQDFSRI 316
           ++  +L  G   RDI+T++A ENA AVV A GGSTNA LHL AIA+EA +   L D + +
Sbjct: 243 LMRAVLGGGPLPRDIVTRKALENACAVVAATGGSTNAPLHLPAIANEAGIDFDLDDVAAV 302

Query: 317 GSGVPHLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDCLTVTGHTMAENLAAIT 376
            +  P +AD+KP GR +  DV  IGG PV++KALLD G LHGDC+TVTG T+AE+LA + 
Sbjct: 303 LARTPLIADLKPGGRFLAKDVFEIGGTPVILKALLDGGHLHGDCMTVTGRTLAEDLAKVC 362

Query: 377 PPDPDGKVLRALANPIHPSGGITILHGSLAPEGAVVKTAGFDSDVFEGTARVFDGERAAL 436
           PPD  G V+R    PI PSGG+T+L G+LAP+GA++KTAG       G ARVF+ E   L
Sbjct: 363 PPD--GAVVRDCGMPIAPSGGVTVLKGNLAPDGALLKTAGLKKLEHRGPARVFESEEQCL 420

Query: 437 DALEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGKDVLLLTDGRFSGGTTG 496
            A+       GD +VIR EGP+GGPGMREML IT  + G G+G+ V L+TDGRFSG T G
Sbjct: 421 SAVRAMAYAAGDVIVIRNEGPRGGPGMREMLGITALLYGQGMGEKVALITDGRFSGATRG 480

Query: 497 LCVGHIAPEAVDGGPIALLRNGDRIRLDVAGRV--LDVLADPAEFASRQQDFSPPPPRYT 554
           +C+G+I+PEA   GPI L+R+GD I +D    +  L V     E  +R+    P   R  
Sbjct: 481 MCIGYISPEAASDGPIGLVRDGDLIAIDARDGIVSLQVELPKGELEARRALPRPARVRPK 540

Query: 555 TGVLSKYVKLVSSAAVGAV 573
            GVL KY  +V  A  GAV
Sbjct: 541 GGVLEKYAAIVGPANKGAV 559


Lambda     K      H
   0.318    0.136    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1043
Number of extensions: 54
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 575
Length of database: 571
Length adjustment: 36
Effective length of query: 539
Effective length of database: 535
Effective search space:   288365
Effective search space used:   288365
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis