Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (characterized)
to candidate YP_001328388.1 Smed_2723 dihydroxy-acid dehydratase
Query= SwissProt::Q1MIB2 (612 letters) >NCBI__GCF_000017145.1:YP_001328388.1 Length = 612 Score = 1144 bits (2960), Expect = 0.0 Identities = 569/612 (92%), Positives = 588/612 (96%) Query: 1 MPVYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV 60 MP YRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV Sbjct: 1 MPAYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV 60 Query: 61 AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCIS 120 AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRE+IADSVEYMVNAHCADAMVCIS Sbjct: 61 AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSREIIADSVEYMVNAHCADAMVCIS 120 Query: 121 NCDKITPGMLMASLRLNIPTVFVSGGPMEAGKVVMHGKTHALDLVDAMVAAADDKISDED 180 NCDKITPGMLMA+LRLNIP VFVSGGPMEAGKVV+HGKTHALDLVDAMVAAADDK+SDED Sbjct: 121 NCDKITPGMLMAALRLNIPAVFVSGGPMEAGKVVLHGKTHALDLVDAMVAAADDKVSDED 180 Query: 181 VQTIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHLDRKRLFVEAGHLIV 240 VQ IERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATH DRKRLFVEAGHLIV Sbjct: 181 VQIIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHADRKRLFVEAGHLIV 240 Query: 241 DLARRYYEQDDVKALPRTIASKQAFENAMTLDIAMGGSTNTVLHILAAAHEGEIDFTMAD 300 D+ARRYYEQ+D + LPR+IASKQAFENAM LDIAMGGSTNTVLHILAAA+EGEIDFTM D Sbjct: 241 DIARRYYEQEDERVLPRSIASKQAFENAMALDIAMGGSTNTVLHILAAAYEGEIDFTMDD 300 Query: 301 IDALSRRVPCLSKVAPAKSDVHMEDVHRAGGIMSILGELDKGGLLNRDCPTVHAETLGDA 360 ID LSR+VPCLSKVAPAK+DVHMEDVHRAGGIMSILGELDKGGL+NRDCPTVHAETLGDA Sbjct: 301 IDRLSRKVPCLSKVAPAKADVHMEDVHRAGGIMSILGELDKGGLINRDCPTVHAETLGDA 360 Query: 361 IDRWDITRTNSETVRKFYRAAPGGIPTQVAFSQEARWDELDTDRENGVIRSVEHPFSKDG 420 IDRWDITRT+SETVR F+RAAPGGIPTQ AFSQ ARWDELDTDR+NGVIRSVEHPFSKDG Sbjct: 361 IDRWDITRTSSETVRNFFRAAPGGIPTQTAFSQAARWDELDTDRQNGVIRSVEHPFSKDG 420 Query: 421 GLAVLKGNLAIDGCIVKTAGVDESILKFSGPARVFESQDASVKAILANEVKAGDVVVIRY 480 GLAVLKGN+A+DGCIVKTAGVDESILKFSGPARVFESQD++VK ILANEVKAGDVVVIRY Sbjct: 421 GLAVLKGNIALDGCIVKTAGVDESILKFSGPARVFESQDSAVKGILANEVKAGDVVVIRY 480 Query: 481 EGPKGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHASPEAANGGTIGL 540 EGPKGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGH SPEAANGGTIGL Sbjct: 481 EGPKGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHVSPEAANGGTIGL 540 Query: 541 VREGDMIDIDIPNRTISLRVSETELAARRAEQDAKGWYPTEVRKRNVTTALKAYAAFATS 600 VREGDMIDIDIPNRTI LRV E ELAARR EQDAKGW P E RKR VTTALKAYAAFATS Sbjct: 541 VREGDMIDIDIPNRTIVLRVDEAELAARRKEQDAKGWKPVEQRKRRVTTALKAYAAFATS 600 Query: 601 ADRGAVRDLNVR 612 ADRGAVRDL R Sbjct: 601 ADRGAVRDLGDR 612 Lambda K H 0.318 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1352 Number of extensions: 26 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 612 Length of database: 612 Length adjustment: 37 Effective length of query: 575 Effective length of database: 575 Effective search space: 330625 Effective search space used: 330625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate YP_001328388.1 Smed_2723 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.5957.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.7e-259 846.4 3.5 5.4e-259 846.1 3.5 1.0 1 lcl|NCBI__GCF_000017145.1:YP_001328388.1 Smed_2723 dihydroxy-acid dehydra Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000017145.1:YP_001328388.1 Smed_2723 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 846.1 3.5 5.4e-259 5.4e-259 2 542 .. 18 608 .. 17 609 .. 0.99 Alignments for each domain: == domain 1 score: 846.1 bits; conditional E-value: 5.4e-259 TIGR00110 2 arallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiamg 70 ar l++atG+kd d++kPiiavvns+t++vPghvhlkdl++lv++eieaaGgvakefntiav+DGiamg lcl|NCBI__GCF_000017145.1:YP_001328388.1 18 ARGLWRATGMKDSDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMG 86 689****************************************************************** PP TIGR00110 71 heGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktkl 139 h+Gm+ysLpsreiiaDsve++v+ah++Da+v+is+CDki+PGmlmaalrlniPa++vsGGpmeagk+ l lcl|NCBI__GCF_000017145.1:YP_001328388.1 87 HDGMLYSLPSREIIADSVEYMVNAHCADAMVCISNCDKITPGMLMAALRLNIPAVFVSGGPMEAGKVVL 155 ********************************************************************* PP TIGR00110 140 sekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstllat 208 k + +d+++a++++a++k+s+e+++ iersacPt+gsCsG+ftansm+cltealGlslPg++++lat lcl|NCBI__GCF_000017145.1:YP_001328388.1 156 HGKTHALDLVDAMVAAADDKVSDEDVQIIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLAT 224 ********************************************************************* PP TIGR00110 209 saekkelakksgkrivelvkknik.......PrdiltkeafenaitldlalGGstntvLhllaiakeag 270 +a++k+l+ ++g+ iv++ +++++ Pr+i++k+afena++ld+a+GGstntvLh+la+a e + lcl|NCBI__GCF_000017145.1:YP_001328388.1 225 HADRKRLFVEAGHLIVDIARRYYEqedervlPRSIASKQAFENAMALDIAMGGSTNTVLHILAAAYEGE 293 ********************************************************************* PP TIGR00110 271 vklslddfdrlsrkvPllaklkPsgkkv.iedlhraGGvsavlkeldkegllhkdaltvtGktlaetle 338 +++++dd+drlsrkvP+l+k++P+ v +ed+hraGG++++l+eldk gl+++d+ tv +tl++ ++ lcl|NCBI__GCF_000017145.1:YP_001328388.1 294 IDFTMDDIDRLSRKVPCLSKVAPAKADVhMEDVHRAGGIMSILGELDKGGLINRDCPTVHAETLGDAID 362 ************************98888**************************************** PP TIGR00110 339 kvkvlr...................................vdqdvirsldnpvkkegglavLkGnlae 372 + +++r ++ virs+++p++k+gglavLkGn+a lcl|NCBI__GCF_000017145.1:YP_001328388.1 363 RWDITRtssetvrnffraapggiptqtafsqaarwdeldtdRQNGVIRSVEHPFSKDGGLAVLKGNIAL 431 *****9*******************************77655555************************ PP TIGR00110 373 eGavvkiagveedilkfeGpakvfeseeealeailggkvkeGdvvviryeGPkGgPGmremLaPtsalv 441 +G++vk+agv+e+ilkf Gpa+vfes++ a+++il+ +vk+GdvvviryeGPkGgPGm+emL+Pts+l+ lcl|NCBI__GCF_000017145.1:YP_001328388.1 432 DGCIVKTAGVDESILKFSGPARVFESQDSAVKGILANEVKAGDVVVIRYEGPKGGPGMQEMLYPTSYLK 500 ********************************************************************* PP TIGR00110 442 glGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialvedGDkikiDienrkldlevseeelaerr 510 + GLgk++aLitDGrfsGgt+GlsiGhvsPeaa+gG+i+lv++GD+i iDi+nr++ l+v+e+ela+rr lcl|NCBI__GCF_000017145.1:YP_001328388.1 501 SKGLGKACALITDGRFSGGTSGLSIGHVSPEAANGGTIGLVREGDMIDIDIPNRTIVLRVDEAELAARR 569 ********************************************************************* PP TIGR00110 511 akakkkea.......revkgaLakyaklvssadkGavld 542 +++++k++ r+v aL++ya +++sad+Gav+d lcl|NCBI__GCF_000017145.1:YP_001328388.1 570 KEQDAKGWkpveqrkRRVTTALKAYAAFATSADRGAVRD 608 *******999**9998999******************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (612 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.02s 00:00:00.05 Elapsed: 00:00:00.04 # Mc/sec: 7.72 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory