Alignments for a candidate for thrC in Crocosphaera subtropica ATCC 51142

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrC in Crocosphaera subtropica ATCC 51142

Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate WP_009544262.1 CCE_RS06870 threonine synthase

Query= BRENDA::O66740
         (352 letters)



>NCBI__GCF_000017845.1:WP_009544262.1
          Length = 376

 Score =  432 bits (1110), Expect = e-126
 Identities = 212/351 (60%), Positives = 271/351 (77%), Gaps = 4/351 (1%)

Query: 4   WQGIIKQYKKYLPVDENTPIVTLYEGNTPLIEADNLARAIGFKGKIYLKYEGLNPTGSFK 63
           W G+I+ Y+ YLPV ENTP+VTL EGNTPLI    +++ +G   K+++KY+GLNPTGSFK
Sbjct: 28  WLGLIETYRPYLPVTENTPVVTLLEGNTPLIPVPYISQQVGKGVKVFVKYDGLNPTGSFK 87

Query: 64  DRGMTLAISKAVEAGKRAVICASTGNTSASAAAYAARAGLRAYVLLPKGAVAIGKLSQAM 123
           DRGMT+AISKA E G +AVICASTGNTSA+AAAYA RAG+RA+V++P G VA+GKL+QA+
Sbjct: 88  DRGMTMAISKAKEDGAKAVICASTGNTSAAAAAYARRAGMRAFVIIPDGYVALGKLAQAL 147

Query: 124 IYGAKVLAIQGTFDDALNIVRKIGENFPVEIVNSVNPYRIEGQKTAAFEICDTLGEAPDY 183
           +YGA+V+AI G FDDAL +V+++ EN+PV +VNSVNPYR+EGQKTAAFE+ D LG APD+
Sbjct: 148 LYGAEVIAIDGNFDDALTVVKQMAENYPVTLVNSVNPYRLEGQKTAAFEVVDVLGNAPDW 207

Query: 184 HFIPVGNAGNITAYWKGFKIYYEEGKITKLPRMMGWQAEGAAPIVKGYPIKNPQTIATAI 243
             IPVGNAGNI+AYW GF  Y+  GK  KLP+MMG+QA GAAP + G P+  P+T+ATAI
Sbjct: 208 LCIPVGNAGNISAYWMGFCQYHSLGKTNKLPKMMGFQAAGAAPFIGGQPVAKPETLATAI 267

Query: 244 KIGNPYSWKSALKAAQESGGKIDAVSDSEILYAYKLIASTEGVFCEPASAASVAGLIKLV 303
           +IGNP +W  A  A   S G+ +AV+D EIL AY+L+A+ EGVFCEPASAASVAGL+K+ 
Sbjct: 268 RIGNPANWDKAWGAKDASQGEFNAVTDEEILDAYRLLAAHEGVFCEPASAASVAGLLKV- 326

Query: 304 REGFFKGGEVVTCTLTGNGLKDPDTAIKVCEEPIT--VPPDFDEVVKVLGF 352
            +        V C LTGNGLKDPD AI+     +   + P+  +V KV+GF
Sbjct: 327 -KDRVPTEATVVCVLTGNGLKDPDCAIQHSNNQVKSGIKPELSQVAKVMGF 376


Lambda     K      H
   0.317    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 451
Number of extensions: 15
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 352
Length of database: 376
Length adjustment: 29
Effective length of query: 323
Effective length of database: 347
Effective search space:   112081
Effective search space used:   112081
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_009544262.1 CCE_RS06870 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00260.hmm
# target sequence database:        /tmp/gapView.22364.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00260  [M=340]
Accession:   TIGR00260
Description: thrC: threonine synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.1e-108  349.4   0.0   1.3e-108  349.2   0.0    1.0  1  lcl|NCBI__GCF_000017845.1:WP_009544262.1  CCE_RS06870 threonine synthase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000017845.1:WP_009544262.1  CCE_RS06870 threonine synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  349.2   0.0  1.3e-108  1.3e-108       1     340 []      32     349 ..      32     349 .. 0.98

  Alignments for each domain:
  == domain 1  score: 349.2 bits;  conditional E-value: 1.3e-108
                                 TIGR00260   1 vvryreilevt.ekdlvdlaegstelfrspklaeevga.enlyvkelfhgPtlaFKDrglqfvavlltk 67 
                                               +++yr +l+vt ++++v+l eg+t+l+ +p ++++vg+  +++vk ++++Pt++FKDrg   ++++++k
  lcl|NCBI__GCF_000017845.1:WP_009544262.1  32 IETYRPYLPVTeNTPVVTLLEGNTPLIPVPYISQQVGKgVKVFVKYDGLNPTGSFKDRG---MTMAISK 97 
                                               689********9***********************************************...******* PP

                                 TIGR00260  68 alelgnetvlcAtsGdtgaaaaealagkanvkvvvLyPkgkispvkeklvtalaenakvlaikGdFDda 136
                                               a+e+g+++v+cA++G+t+aaaa a+a++a+++++v++P+g+++   +kl++al ++a+v+ai+G+FDda
  lcl|NCBI__GCF_000017845.1:WP_009544262.1  98 AKEDGAKAVICASTGNTSAAAA-AYARRAGMRAFVIIPDGYVA--LGKLAQALLYGAEVIAIDGNFDDA 163
                                               **********************.********************..************************ PP

                                 TIGR00260 137 qdlvkeifedkeklklnsvNsinparieaqktyafeiveqlgkespdkvvvpvp.sgnfgailkGflek 204
                                               +++vk+++e+ + +++nsvN   p+r+e+qkt+afe+v+ lg ++pd++++pv+ +gn++a+++Gf+++
  lcl|NCBI__GCF_000017845.1:WP_009544262.1 164 LTVVKQMAENYPVTLVNSVN---PYRLEGQKTAAFEVVDVLG-NAPDWLCIPVGnAGNISAYWMGFCQY 228
                                               ********988888888888...*******************.************************** PP

                                 TIGR00260 205 kelg....lpieklaiaaegaadivrrflksgdlepkedkeTlstAmdignpsnveralelarrslgnl 269
                                               ++lg    lp ++++++a gaa+++  +       p +++eTl+tA++ignp+n+++a+ +   s+g++
  lcl|NCBI__GCF_000017845.1:WP_009544262.1 229 HSLGktnkLP-KMMGFQAAGAAPFIGGQ-------PVAKPETLATAIRIGNPANWDKAWGAKDASQGEF 289
                                               **********.**********9988777.......9********************************* PP

                                 TIGR00260 270 edlkesvsdeeileaikklaeeegyllephtavavaalkklvekgvsatadpaKFeevvealtgnklkd 338
                                               +++    +deeil+a+++la++eg+++ep++a++va+l+k  +     +++ +  ++vv++ltgn+lkd
  lcl|NCBI__GCF_000017845.1:WP_009544262.1 290 NAV----TDEEILDAYRLLAAHEGVFCEPASAASVAGLLKVKD-----RVPTE--ATVVCVLTGNGLKD 347
                                               ***....*********************************987.....78***..************** PP

                                 TIGR00260 339 pe 340
                                               p+
  lcl|NCBI__GCF_000017845.1:WP_009544262.1 348 PD 349
                                               97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (340 nodes)
Target sequences:                          1  (376 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 8.45
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis