Alignments for a candidate for ilvI in Crocosphaera subtropica ATCC 51142

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Crocosphaera subtropica ATCC 51142

Align Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate WP_009543700.1 CCE_RS19930 acetolactate synthase large subunit

Query= curated2:O08353
         (599 letters)



>NCBI__GCF_000017845.1:WP_009543700.1
          Length = 550

 Score =  347 bits (890), Expect = e-100
 Identities = 206/554 (37%), Positives = 298/554 (53%), Gaps = 29/554 (5%)

Query: 1   MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARA 60
           MN AE ++K LE E VE +FG PG   L   +AL  S +  + TRHEQ AA  AD Y R 
Sbjct: 4   MNTAELLVKCLENEGVEYIFGLPGEENLHILEALKDSSIQFITTRHEQGAAFMADVYGRL 63

Query: 61  SGKVGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLFMP 120
           +GK GVC+ T GPGATNL+TGVA A+ D +P+VA+TGQV T  +  ++ Q +D + +F P
Sbjct: 64  TGKAGVCLSTLGPGATNLMTGVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFAP 123

Query: 121 IVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDIDKHPIPSKVKLI 180
           + K N QI +    PE+ R AF++AQ+ +PG VHIDLP+++  L       P+       
Sbjct: 124 VTKWNKQIVRPSITPEVTRKAFKLAQSEKPGAVHIDLPENIAAL-------PVEGWPLTR 176

Query: 181 GYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTLMG 240
                T    R +  A   I+ AK P+IL G G + + A+E L +    LNIPV  T MG
Sbjct: 177 DSQEKTYASYRSLNAAAMAISKAKNPLILVGNGTIRAHASEALTEFATTLNIPVANTFMG 236

Query: 241 KGCISENHPLALGMVGMHGTKPANYCLSESDVLISIGCRFSDRITGDIKSF--ATNAKII 298
           KG +   HPL+L  VG+           +SD++I++G    D I    K +       II
Sbjct: 237 KGAVPFTHPLSLWTVGLQQRDLITCAFEQSDLVIAVG---YDLIEYSPKKWNPEGTTPII 293

Query: 299 HIDIDPAEIGKNVNVDVPIVGDAKLILKEVIKQLDYIINKDSKENNDKENISQWIENVNS 358
           HI + PAEI  +    V +VGD    L +++K+ D    +  K  +    +       + 
Sbjct: 294 HIGMTPAEIDSSYIPLVEVVGDISDSLMDILKRAD----RQGKSTSVAAGLR------SD 343

Query: 359 LKKSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQNQMWMAHYFKTQTP 418
           L +        +  P+KPQKI+ +L  V     +    I+ +DVG ++MWMA ++   TP
Sbjct: 344 LVREYEKYAHDEGFPVKPQKIIYDLRQV-----MGPEDIVISDVGAHKMWMARHYHCDTP 398

Query: 419 RSFLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNIPVVIC 478
            + L S G   MG   P AI AK+  PD KV+ +TGDGGFMMNCQEL T      P V  
Sbjct: 399 NTCLISNGFAAMGIAIPGAIAAKLVAPDKKVVAVTGDGGFMMNCQELETALRAGTPFVTL 458

Query: 479 IFDNRTLGMVYQWQNLFYGKRQCSVNFGGAPDFIKLAESYGIKARRIESPNEINEALKEA 538
           IF++   G++ +W+ +    +   + F   PDF+K AES G+K  R+ES +++   LK A
Sbjct: 459 IFNDNGYGLI-EWKQINQFGQSSFIKFTN-PDFVKFAESMGLKGYRVESADDLVPTLKTA 516

Query: 539 INCDEPYLLDFAID 552
           +    P ++D  +D
Sbjct: 517 LEQGVPAVIDCPVD 530


Lambda     K      H
   0.319    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 763
Number of extensions: 27
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 599
Length of database: 550
Length adjustment: 36
Effective length of query: 563
Effective length of database: 514
Effective search space:   289382
Effective search space used:   289382
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis