Align homoserine dehydrogenase (EC 1.1.1.3); aspartate kinase (EC 2.7.2.4) (characterized)
to candidate WP_012282995.1 HM1_RS08710 homoserine dehydrogenase
Query= BRENDA::Q9WZ17 (739 letters) >NCBI__GCF_000019165.1:WP_012282995.1 Length = 429 Score = 207 bits (528), Expect = 7e-58 Identities = 140/427 (32%), Positives = 223/427 (52%), Gaps = 12/427 (2%) Query: 20 VRVGIAGLGTVGGSIYRILKERGNEIEKRIGEKFIISKVINRSPQKYELLGVPKEEIAFD 79 + VG+ GLGTVGG RIL+ R +I +R+G + KV+ R+ K + V + + Sbjct: 5 IMVGLLGLGTVGGGTLRILQNRVEDIAQRLGRPVQVKKVLVRNLHKPRPVVVDPALLTDN 64 Query: 80 FDDLILNSDV--VVEAIGGTDVAVDLVRRALELGRIVVTPNKNLISEYGNEFSEYIKKRK 137 D++ + ++ VVE +GG A+D ++ AL+ G+ VVT NK+L++ +G E + + ++ Sbjct: 65 PADILEDPEISIVVELMGGIHPALDYIKSALKAGKNVVTANKDLMAVHGRELFDLAEAKE 124 Query: 138 LF--FEASVGGGIPIISLLQDYLIFQKVTRIRGIMNGTTNYILTEMSK-GRHFEEVLKEA 194 L FEASV GGIPII L+ L ++ ++GI+NGTTN+ILT+M++ G F EVLKEA Sbjct: 125 LDLEFEASVAGGIPIIRPLKVCLAANRIEELKGIINGTTNFILTKMTQEGSDFGEVLKEA 184 Query: 195 QELGYAEADPTNDIEGYDVAYKVSVLAGVVTGRFPGINSVQFEGITRIDPEYLKEIVRSG 254 Q LGYAEADPT D+EG D A K++++A + +N V EGIT++ ++ + G Sbjct: 185 QALGYAEADPTADVEGLDAARKLAIVASIAFNSRITLNDVYVEGITKVTARDIQYARQMG 244 Query: 255 KKLKLIGELDFSTNRYEVRLREVTPED--PFFNVDGVDNAIEVSTDLAGDFLLKGRGAGG 312 +KL+G + E R+ D P V+ NAI V D G+ + GRGAG Sbjct: 245 YTIKLLGIAKEIDGKVEARVHPAMIPDCHPLAAVNDAFNAIFVKGDAVGETMFYGRGAGD 304 Query: 313 YPTASAVIADLFRVAKYKVLGGAEKFSVVVMKFGGAAISDVEKLEKVAEKIIKRKKSGVK 372 PT SAV+ D+ VA+ + G + S V + ++ K R K Sbjct: 305 MPTGSAVVGDIIDVARNIIKGHTGRISCTCYH-----QKPVRPIGEIVCKYFLRLVVTEK 359 Query: 373 PVVVLSAMGDTTDHLIELAKTIDENPDPRELDLLLSTGEIQSVALMSIALRKRGYKAISF 432 P V+ + G D + + I + +++L T ++ L ++ Sbjct: 360 PGVLAAIAGVFGDQGVSIQTLIQQETIGDSAEIVLVTHPVREDNLQMALKILSDMPSVER 419 Query: 433 TGNQLKI 439 GN +++ Sbjct: 420 VGNVIRV 426 Score = 26.6 bits (57), Expect = 0.003 Identities = 13/40 (32%), Positives = 19/40 (47%) Query: 605 VPDKPGVAARIMRTLSQMGVNIDMIIQGMKSGEYNTVAFI 644 V +KPGV A I GV+I +IQ G+ + + Sbjct: 356 VTEKPGVLAAIAGVFGDQGVSIQTLIQQETIGDSAEIVLV 395 Lambda K H 0.318 0.137 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 741 Number of extensions: 33 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 739 Length of database: 429 Length adjustment: 36 Effective length of query: 703 Effective length of database: 393 Effective search space: 276279 Effective search space used: 276279 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory