Alignments for a candidate for gatB in Methylobacterium sp. 4-46

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatB in Methylobacterium sp. 4-46

Align Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.- (characterized)
to candidate WP_012334068.1 M446_RS20740 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit B

Query= SwissProt::Q9RF06
         (475 letters)



>NCBI__GCF_000019365.1:WP_012334068.1
          Length = 490

 Score =  400 bits (1029), Expect = e-116
 Identities = 215/473 (45%), Positives = 310/473 (65%), Gaps = 6/473 (1%)

Query: 3   FETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAMRA 62
           +E VIG+E+H ++ + SK+FS +   FGAEPN++ +++D A PG+LPV+N+  V  A+R 
Sbjct: 18  WEVVIGMEIHAQVTSRSKLFSGASTAFGAEPNAHVSLVDAAMPGMLPVINRECVAQAVRT 77

Query: 63  AMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEV-DGETKRIGITRL 121
            + L  +I   S FDRKNYFYPD P+ YQISQ+  PI   G + +++ DGET R+GI RL
Sbjct: 78  GLGLKAQINHRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGEVLVDLPDGETIRVGIERL 137

Query: 122 HMEEDAGKSTHKGEYSL--VDLNRQGTPLIEIVSEPDIRSPKEAYAYLEKLRSIIQYTGV 179
           H+E+DAGKS H    SL  VDLNR G  L+EIVS+PD+RS +EA AY+ KLR+I++Y G 
Sbjct: 138 HLEQDAGKSLHDQHPSLSFVDLNRSGVALMEIVSKPDLRSSEEAKAYVTKLRTILRYLGT 197

Query: 180 SDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLSGG 239
            D  ME+G+LR D N+S+R  G E FGT+ E+KN+NS  ++ + +E E +RQ   L  GG
Sbjct: 198 CDGDMEKGNLRADVNVSVRRPG-EPFGTRCEIKNVNSIRFIGQAIETEARRQIAILEDGG 256

Query: 240 EIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKERVRQTIPELPDER 299
            I QETR +D +  +T  MR KE + DYRYFP+PD++PL I+ A+ + +R  +PELPD +
Sbjct: 257 WIEQETRLYDPNRNETRSMRSKEEAHDYRYFPDPDLLPLEIEQAFIDGLRTELPELPDAK 316

Query: 300 KAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQVEL 359
           KA++V E GL AYDA VL   +  +D+FE+ +  G D K  +NW++  +   LNK    +
Sbjct: 317 KARFVAEYGLSAYDATVLVAERASADYFEA-VAKGRDGKAAANWVINELFGRLNKEGHGI 375

Query: 360 LDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGLVQISDEATLL 419
             + ++   L  +I LI DGT+S KIAK +F  +  +GG+ + I+E  GL Q++D   + 
Sbjct: 376 EGSPVSAAQLGAIIDLIADGTISGKIAKDLFEIVWGEGGDPRAIVESRGLKQVTDTGAIE 435

Query: 420 KFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQEL 472
             V++ +  N   V   K  K   +G+ VGQ MKA+ G+ANP  VN LLK +L
Sbjct: 436 AAVDQIIAANPDKVAQAK-AKPTLLGWFVGQTMKATGGKANPAAVNALLKTKL 487


Lambda     K      H
   0.313    0.133    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 633
Number of extensions: 27
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 475
Length of database: 490
Length adjustment: 34
Effective length of query: 441
Effective length of database: 456
Effective search space:   201096
Effective search space used:   201096
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)

Align candidate WP_012334068.1 M446_RS20740 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit B)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00133.hmm
# target sequence database:        /tmp/gapView.4771.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00133  [M=481]
Accession:   TIGR00133
Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.7e-163  530.5   0.0   2.1e-163  530.2   0.0    1.0  1  lcl|NCBI__GCF_000019365.1:WP_012334068.1  M446_RS20740 Asp-tRNA(Asn)/Glu-t


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000019365.1:WP_012334068.1  M446_RS20740 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit B
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  530.2   0.0  2.1e-163  2.1e-163       3     480 ..      17     487 ..      16     488 .. 0.97

  Alignments for each domain:
  == domain 1  score: 530.2 bits;  conditional E-value: 2.1e-163
                                 TIGR00133   3 eyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalnsk 71 
                                               ++e+viG+E+H q+  +sKlF+ +s+ +   +pN +v+ v+ ++PG lPv+N+e v++A+  +l l+++
  lcl|NCBI__GCF_000019365.1:WP_012334068.1  17 DWEVVIGMEIHAQVTSRSKLFSGASTAFGA-EPNAHVSLVDAAMPGMLPVINRECVAQAVRTGLGLKAQ 84 
                                               5799************************99.************************************** PP

                                 TIGR00133  72 ivsevsvFdRKhYfYpDlPkgyqitqqdlPiaedGkleieleeke.keigierlhlEeDtgksqykesd 139
                                               i+ + svFdRK+YfYpDlP+gyqi+q++ Pi+ +G++ ++l ++e  ++gierlhlE+D+gks + ++ 
  lcl|NCBI__GCF_000019365.1:WP_012334068.1  85 IN-HRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGEVLVDLPDGEtIRVGIERLHLEQDAGKSLHDQH- 151
                                               66.69************************************9988579*****************966. PP

                                 TIGR00133 140 kdkqslvDfNRsgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlkG 208
                                                ++ s+vD+NRsgv L+EiV+kPdl+s++ea+a++ klr+ilryl+  dgd+e+G++R+DvNvs+r   
  lcl|NCBI__GCF_000019365.1:WP_012334068.1 152 -PSLSFVDLNRSGVALMEIVSKPDLRSSEEAKAYVTKLRTILRYLGTCDGDMEKGNLRADVNVSVRRP- 218
                                               .79***************************************************************55. PP

                                 TIGR00133 209 qekegtrvEiKNlnslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRYf 277
                                                e +gtr EiKN+ns++ i +aie E  Rq+++l++g  ++qetr +d +++ t s+R+Kee++DYRYf
  lcl|NCBI__GCF_000019365.1:WP_012334068.1 219 GEPFGTRCEIKNVNSIRFIGQAIETEARRQIAILEDGGWIEQETRLYDPNRNETRSMRSKEEAHDYRYF 287
                                               6789***************************************************************** PP

                                 TIGR00133 278 pePdlppieideevvkekveeklpelPeakrirlkkeyglseedakvlvsdlelldafeevvklikepk 346
                                               p+Pdl p+ei++ ++++ ++++lpelP+ak++r++ eygls++da vlv+++  +d+fe v+k  +  k
  lcl|NCBI__GCF_000019365.1:WP_012334068.1 288 PDPDLLPLEIEQAFIDG-LRTELPELPDAKKARFVAEYGLSAYDATVLVAERASADYFEAVAKGRDG-K 354
                                               *****************.*****************************************99998776.* PP

                                 TIGR00133 347 lavnWileellgeLnkkkislaeallkpeelaeliklikegkisqksakelleellenkkdpkkliekl 415
                                               +a+nW+++el g+Lnk+   ++ + +++ +l ++i+li +g+is+k+ak+l+e +  +++dp++++e +
  lcl|NCBI__GCF_000019365.1:WP_012334068.1 355 AAANWVINELFGRLNKEGHGIEGSPVSAAQLGAIIDLIADGTISGKIAKDLFEIVWGEGGDPRAIVESR 423
                                               ********************************************************************* PP

                                 TIGR00133 416 gliqisdekelvkiveevikenpkevekyksgkekalkflvGqvmkktkgradpkevekllkell 480
                                               gl q++d+ ++ ++v+++i+ np++v + k  k ++l+++vGq mk t g+a+p+ v++llk +l
  lcl|NCBI__GCF_000019365.1:WP_012334068.1 424 GLKQVTDTGAIEAAVDQIIAANPDKVAQAK-AKPTLLGWFVGQTMKATGGKANPAAVNALLKTKL 487
                                               **************************9876.5789**************************9987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (481 nodes)
Target sequences:                          1  (490 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 9.69
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis