Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (characterized)
to candidate WP_012334770.1 M446_RS24325 dihydroxy-acid dehydratase
Query= SwissProt::P9WKJ5 (575 letters) >NCBI__GCF_000019365.1:WP_012334770.1 Length = 577 Score = 550 bits (1418), Expect = e-161 Identities = 295/555 (53%), Positives = 370/555 (66%), Gaps = 8/555 (1%) Query: 21 SRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNEITPCNLSLDRLANAVKEGVF 80 SR VT+G +A R L A+G+ E +P +GVAS WNE PCN++L R A AVK+GV Sbjct: 14 SRHVTEGPARAPHRSYLYAMGLTREQIHQPLVGVASCWNEAAPCNIALMRQAQAVKKGVA 73 Query: 81 SAGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVEVVMQAERLDGSVLLAGCDKS 140 +A G P EF TI+V+DGI+MGH GM SL SREVIADSVE+ M+ D V LAGCDKS Sbjct: 74 AAAGTPREFCTITVTDGIAMGHRGMRASLPSREVIADSVELTMRGHAYDALVGLAGCDKS 133 Query: 141 LPGMLMAAARLDLAAVFLYAGSILPGRAKLSDGSERDVTIIDAFEAVGACSRGLMSRADV 200 LPGM+MA RL++ ++F+Y GSILPG + R VT+ D FEAVG S G MS D+ Sbjct: 134 LPGMMMAMVRLNVPSIFIYGGSILPGTFR-----GRPVTVQDLFEAVGKHSVGAMSDEDL 188 Query: 201 DAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAPPATDRRRDGFARRSGQAVVE 260 D +E+ CP GACG +TANTMA+ +EA+G++LP SA PA RD F +G+ V++ Sbjct: 189 DELEQVACPSAGACGAQFTANTMATVSEAIGLALPYSAGAPAPYEIRDKFCAAAGEMVMD 248 Query: 261 LLRRGITARDILTKEAFENAIAVVMAFGGSTNAVLHLLAIAHEANVALSLQDFSRIGSGV 320 LL R I RDI+T+ A ENA VV A GGSTNA LHL AIAHEA ++ L D + I Sbjct: 249 LLARNIRPRDIVTRRALENAATVVAASGGSTNAALHLPAIAHEAGISFDLFDVAEIFKRT 308 Query: 321 PHLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDCLTVTGHTMAENLAAITPPDP 380 P++AD+KP GR+V D+ +GG+P++MK LLD G LHGDC+TVTG T+AENLA + D Sbjct: 309 PYVADLKPGGRYVAKDLFEVGGIPLLMKTLLDHGFLHGDCMTVTGRTIAENLAKVAWNDQ 368 Query: 381 DGKVLRALANPIHPSGGITILHGSLAPEGAVVKTAGF--DSDVFEGTARVFDGERAALDA 438 V+R PI P+GG+ L G+LAPEGA+VK AG D VF G ARVFD E A +A Sbjct: 369 Q-DVVRPANTPITPTGGVVGLKGNLAPEGAIVKVAGMAPDRQVFAGPARVFDTEEACFEA 427 Query: 439 LEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGKDVLLLTDGRFSGGTTGLC 498 +++ GD +VIRYEGPKGGPGMREMLA T A+ G G+G V L+TDGRFSG T G C Sbjct: 428 VQNRQYKEGDVLVIRYEGPKGGPGMREMLATTAALYGQGMGDKVALITDGRFSGATRGFC 487 Query: 499 VGHIAPEAVDGGPIALLRNGDRIRLDVAGRVLDVLADPAEFASRQQDFSPPPPRYTTGVL 558 VGH+ PEA GGPI LL++GD IRLD L V E A R++ ++P T+G L Sbjct: 488 VGHVGPEAAVGGPIGLLKDGDIIRLDAIQGTLTVDLSDEELAERRKAWAPRGNEATSGYL 547 Query: 559 SKYVKLVSSAAVGAV 573 KY + V A GAV Sbjct: 548 WKYAQTVGPAVNGAV 562 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 970 Number of extensions: 47 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 575 Length of database: 577 Length adjustment: 36 Effective length of query: 539 Effective length of database: 541 Effective search space: 291599 Effective search space used: 291599 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate WP_012334770.1 M446_RS24325 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.20894.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.7e-211 689.4 0.3 1.9e-211 689.2 0.3 1.0 1 lcl|NCBI__GCF_000019365.1:WP_012334770.1 M446_RS24325 dihydroxy-acid dehy Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000019365.1:WP_012334770.1 M446_RS24325 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 689.2 0.3 1.9e-211 1.9e-211 1 541 [. 25 563 .. 25 565 .. 0.99 Alignments for each domain: == domain 1 score: 689.2 bits; conditional E-value: 1.9e-211 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 ++r+ l+a+Gl+ e++++P+++v+++++e +P+++ l a++vk+++ aa g++ ef ti+v+DGiam lcl|NCBI__GCF_000019365.1:WP_012334770.1 25 PHRSYLYAMGLTREQIHQPLVGVASCWNEAAPCNIALMRQAQAVKKGVAAAAGTPREFCTITVTDGIAM 93 699****************************************************************** PP TIGR00110 70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138 gh+Gm+ sLpsre+iaDsve +++ha+Dalv ++ CDk +PGm+ma++rln+P+i+++GG++ +g ++ lcl|NCBI__GCF_000019365.1:WP_012334770.1 94 GHRGMRASLPSREVIADSVELTMRGHAYDALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGTFR 162 ********************************************************************* PP TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207 ++ +++ d+feavg+ + g +s+e+l+e+e++acP+ag+C+ ftan+ma+++ea+Gl+lP+s+ ++a lcl|NCBI__GCF_000019365.1:WP_012334770.1 163 -GRPVTVQDLFEAVGKHSVGAMSDEDLDELEQVACPSAGACGAQFTANTMATVSEAIGLALPYSAGAPA 230 .9******************************************************************* PP TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsld 276 + + +++ +g+ +++l+ +ni+Prdi+t++a+ena t++ a GGstn+ Lhl+aia+eag++++l lcl|NCBI__GCF_000019365.1:WP_012334770.1 231 PYEIRDKFCAAAGEMVMDLLARNIRPRDIVTRRALENAATVVAASGGSTNAALHLPAIAHEAGISFDLF 299 ********************************************************************* PP TIGR00110 277 dfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlrv 345 d+ ++ +++P +a+lkP+g++v +dl ++GG++ ++k+l +g lh d++tvtG+t+ae+l+kv + lcl|NCBI__GCF_000019365.1:WP_012334770.1 300 DVAEIFKRTPYVADLKPGGRYVAKDLFEVGGIPLLMKTLLDHGFLHGDCMTVTGRTIAENLAKVAWN-D 367 ****************************************************************999.8 PP TIGR00110 346 dqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeG 414 +qdv+r+ + p++ +gg+ LkGnla+eGa+vk+ag++ d +f Gpa+vf+ ee++ ea+ +++ keG lcl|NCBI__GCF_000019365.1:WP_012334770.1 368 QQDVVRPANTPITPTGGVVGLKGNLAPEGAIVKVAGMAPDRQVFAGPARVFDTEEACFEAVQNRQYKEG 436 99******************************************************************* PP TIGR00110 415 dvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialve 483 dv+viryeGPkGgPGmremLa t+al g G+g+kvaLitDGrfsG+trG+++Ghv Peaa+gG+i+l++ lcl|NCBI__GCF_000019365.1:WP_012334770.1 437 DVLVIRYEGPKGGPGMREMLATTAALYGQGMGDKVALITDGRFSGATRGFCVGHVGPEAAVGGPIGLLK 505 ********************************************************************* PP TIGR00110 484 dGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavl 541 dGD+i++D+ + +l +++s+eelaerr+++ +++++ ++g+L kya+ v a +Gav+ lcl|NCBI__GCF_000019365.1:WP_012334770.1 506 DGDIIRLDAIQGTLTVDLSDEELAERRKAWAPRGNEATSGYLWKYAQTVGPAVNGAVT 563 ********************************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (577 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 11.99 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory