Alignments for a candidate for aroG in Nostoc punctiforme ATCC 29133; PCC 73102

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroG in Nostoc punctiforme ATCC 29133; PCC 73102

Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate WP_012407778.1 NPUN_RS05230 3-deoxy-7-phosphoheptulonate synthase

Query= BRENDA::P00888
         (356 letters)



>NCBI__GCF_000020025.1:WP_012407778.1
          Length = 352

 Score =  418 bits (1075), Expect = e-121
 Identities = 197/342 (57%), Positives = 262/342 (76%)

Query: 6   LNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKSISDIIAGRDPRLLVVCGPCSIH 65
           ++N +I ++++L+TP ++K+  PL+   E ++   R+ I DI+  +D R  +V GPCSIH
Sbjct: 10  IDNANIENDRILLTPNEIKSKLPLTQLAEQRVLAYRQEIEDILDFQDRRKFIVVGPCSIH 69

Query: 66  DPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLQ 125
           DP+ ALEY+ R KAL+ EV D L L+MRVYFEKPRTTVGWKGLINDP MD SF VE GL 
Sbjct: 70  DPKAALEYSERLKALSEEVKDKLLLIMRVYFEKPRTTVGWKGLINDPDMDDSFHVENGLL 129

Query: 126 IARKLLLELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGLSMPVG 185
           IAR LLL++  +GLP  TEALDP  PQY+ +L +WSAIGARTTESQTHREMASGLSMPVG
Sbjct: 130 IARDLLLKITELGLPAGTEALDPIIPQYISELITWSAIGARTTESQTHREMASGLSMPVG 189

Query: 186 FKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKAPNYSPA 245
           FKNGTDG++  A+NA+++A  PH F+GINQ GQV++ QT+GN  GHVILRGG  PN+  A
Sbjct: 190 FKNGTDGNIQVALNALQSARNPHNFLGINQNGQVSVFQTKGNGYGHVILRGGSQPNFDAA 249

Query: 246 DVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESN 305
           +V   E +++QA L P +++DCSHGN+NKDY+ Q AV E+++ QI DGN SI+G+M+ES+
Sbjct: 250 NVKLVEDKLKQANLPPRIVIDCSHGNTNKDYKLQGAVLENIIQQIVDGNTSIVGMMLESH 309

Query: 306 IHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHQDL 347
           ++EG+Q     + E+KYGVSVTD CISWE T+ ++   ++ L
Sbjct: 310 LYEGSQPITDKQEELKYGVSVTDKCISWEETEKIILAAYEKL 351


Lambda     K      H
   0.316    0.131    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 378
Number of extensions: 8
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 356
Length of database: 352
Length adjustment: 29
Effective length of query: 327
Effective length of database: 323
Effective search space:   105621
Effective search space used:   105621
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_012407778.1 NPUN_RS05230 (3-deoxy-7-phosphoheptulonate synthase)
to HMM TIGR00034 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00034.hmm
# target sequence database:        /tmp/gapView.22059.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00034  [M=342]
Accession:   TIGR00034
Description: aroFGH: 3-deoxy-7-phosphoheptulonate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.2e-155  503.4   0.0   1.5e-155  503.1   0.0    1.0  1  lcl|NCBI__GCF_000020025.1:WP_012407778.1  NPUN_RS05230 3-deoxy-7-phosphohe


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000020025.1:WP_012407778.1  NPUN_RS05230 3-deoxy-7-phosphoheptulonate synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  503.1   0.0  1.5e-155  1.5e-155       3     332 ..      13     345 ..      11     352 .] 0.97

  Alignments for each domain:
  == domain 1  score: 503.1 bits;  conditional E-value: 1.5e-155
                                 TIGR00034   3 lrivkidelltPeelkakfpltekaaekvaksrkeiadilaGkddrllvviGPcsihdpeaaleyakrl 71 
                                                +i++   lltP+e+k+k plt+ a+++v   r+ei+dil+ +d r ++v+GPcsihdp+aaley++rl
  lcl|NCBI__GCF_000020025.1:WP_012407778.1  13 ANIENDRILLTPNEIKSKLPLTQLAEQRVLAYRQEIEDILDFQDRRKFIVVGPCSIHDPKAALEYSERL 81 
                                               5555556688*********************************************************** PP

                                 TIGR00034  72 kklaeklkddleivmrvyfekPrttvGWkGlindPdlnesfdvnkGlriarkllldlvelglplatell 140
                                               k+l+e++kd+l ++mrvyfekPrttvGWkGlindPd+++sf+v++Gl iar+lll+++elglp++te+l
  lcl|NCBI__GCF_000020025.1:WP_012407778.1  82 KALSEEVKDKLLLIMRVYFEKPRTTVGWKGLINDPDMDDSFHVENGLLIARDLLLKITELGLPAGTEAL 150
                                               ********************************************************************* PP

                                 TIGR00034 141 dtispqyladllswgaiGarttesqvhrelasglslpvgfkngtdGslkvaidairaaaaehlflsvtk 209
                                               d+i+pqy+++l++w+aiGarttesq+hre+asgls+pvgfkngtdG+++va++a+++a+++h fl++++
  lcl|NCBI__GCF_000020025.1:WP_012407778.1 151 DPIIPQYISELITWSAIGARTTESQTHREMASGLSMPVGFKNGTDGNIQVALNALQSARNPHNFLGINQ 219
                                               ********************************************************************* PP

                                 TIGR00034 210 aGqvaivetkGnedghiilrGGkkpnydaedvaevkeelekaglkeelmidfshgnsnkdykrqlevae 278
                                               +Gqv++ +tkGn +gh+ilrGG +pn+da++v+ v+++l++a+l ++++id+shgn+nkdyk q +v e
  lcl|NCBI__GCF_000020025.1:WP_012407778.1 220 NGQVSVFQTKGNGYGHVILRGGSQPNFDAANVKLVEDKLKQANLPPRIVIDCSHGNTNKDYKLQGAVLE 288
                                               ********************************************************************* PP

                                 TIGR00034 279 svveqiaeGekaiiGvmiesnleeGnqsl...keelkyGksvtdacigwedteallr 332
                                               ++++qi +G+++i+G+m+es+l eG+q +   +eelkyG+svtd+ci+we+te+++ 
  lcl|NCBI__GCF_000020025.1:WP_012407778.1 289 NIIQQIVDGNTSIVGMMLESHLYEGSQPItdkQEELKYGVSVTDKCISWEETEKIIL 345
                                               ***************************98765789******************9985 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (342 nodes)
Target sequences:                          1  (352 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.61
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis