Alignments for a candidate for thrC in Nostoc punctiforme ATCC 29133; PCC 73102

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrC in Nostoc punctiforme ATCC 29133; PCC 73102

Align Threonine synthase; TS; EC 4.2.3.1 (uncharacterized)
to candidate WP_012407541.1 NPUN_RS03905 threonine synthase

Query= curated2:Q58860
         (405 letters)



>NCBI__GCF_000020025.1:WP_012407541.1
          Length = 436

 Score =  278 bits (710), Expect = 3e-79
 Identities = 155/404 (38%), Positives = 240/404 (59%), Gaps = 8/404 (1%)

Query: 4   RCIKCGKTYDVDEIIYTCE-CGGLLEIIYDYEEIKDKVSEEKLRKREIGVWRYLEYLPVK 62
           +C +CG  Y++ +    CE C G LE+ YDY  ++  V+ E ++     +WRY  +LPV 
Sbjct: 22  KCKECGAEYEL-KASNVCELCFGPLEVKYDYSALRLTVTRETIQAGPNSIWRYRPFLPVA 80

Query: 63  DESKIVSLCEGGTPLYRCNNLEKELGIKELYVKNEGAN-PTGSFKDRGMTVGVTRANELG 121
            ++ ++ +  G TPL R + L + LG+ +LY+KN+  N PT SFKDR ++V ++RA ELG
Sbjct: 81  TDN-VIDVGTGMTPLVRSHRLARRLGLNKLYIKNDAVNMPTLSFKDRVVSVALSRARELG 139

Query: 122 VEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVIQVKGNFDD 181
              V CASTGN + S AA +A +G  C V +P    A GK+  ++ Y   ++ VKGN+D 
Sbjct: 140 FTTVSCASTGNLANSTAAIAAHAGLDCCVFIPADLEA-GKIIGSLIYSPTLMAVKGNYDQ 198

Query: 182 ALDMVKQLAKEKLIYLLN-SINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVGNAGNISA 240
              +  ++A       +N ++ P+  EG KT+ FE+ +QL W++PD V+ P+ +    + 
Sbjct: 199 VNRLCSEVANTHGWGFVNINLRPYYSEGSKTLGFEVAEQLGWELPDHVVAPLASGSLFTK 258

Query: 241 IWKGFKEFEITGII-DELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIATAIRIGN 299
           I+KGF+EF   G++ D+  + +G QA+G  PI +AF K  +D I    P TIA ++ IGN
Sbjct: 259 IYKGFQEFIEVGLVEDKKVRFSGAQAEGCSPIAQAF-KEGRDFIKPVKPNTIAKSLAIGN 317

Query: 300 PVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKKLLEEGI 359
           P +   A++    +GG  E+V D EI++  KLLA  EGIF E A  +++A LKKL+E G 
Sbjct: 318 PADGIYAVELAQKTGGNIESVNDAEIIDGIKLLAETEGIFTETAGGTTVAVLKKLVEAGK 377

Query: 360 IDRDERIVCITTGHGLKDPDAAIRASEEPIKIECDMNVLKRILK 403
           ID DE  V   TG+GLK  +A      EP+ I+  ++  +R L+
Sbjct: 378 IDPDETTVIYITGNGLKTQEAIQGYVGEPLTIDAKLDSFERALE 421


Lambda     K      H
   0.317    0.137    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 468
Number of extensions: 28
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 405
Length of database: 436
Length adjustment: 32
Effective length of query: 373
Effective length of database: 404
Effective search space:   150692
Effective search space used:   150692
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

Align candidate WP_012407541.1 NPUN_RS03905 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00260.hmm
# target sequence database:        /tmp/gapView.22843.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00260  [M=340]
Accession:   TIGR00260
Description: thrC: threonine synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   5.6e-105  337.2   0.0   6.6e-105  336.9   0.0    1.1  1  lcl|NCBI__GCF_000020025.1:WP_012407541.1  NPUN_RS03905 threonine synthase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000020025.1:WP_012407541.1  NPUN_RS03905 threonine synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  336.9   0.0  6.6e-105  6.6e-105       1     340 []      70     397 ..      70     397 .. 0.99

  Alignments for each domain:
  == domain 1  score: 336.9 bits;  conditional E-value: 6.6e-105
                                 TIGR00260   1 vvryreilevtekdlvdlaegstelfrspklaeevgaenlyvkelfhg.PtlaFKDrglqfvavlltka 68 
                                               ++ryr +l+v   +++d+ +g+t+l+rs++la+++g+++ly+k+++++ Ptl+FKDr+   v+v+l++a
  lcl|NCBI__GCF_000020025.1:WP_012407541.1  70 IWRYRPFLPVATDNVIDVGTGMTPLVRSHRLARRLGLNKLYIKNDAVNmPTLSFKDRV---VSVALSRA 135
                                               8*********************************************************...******** PP

                                 TIGR00260  69 lelgnetvlcAtsGdtgaaaaealagkanvkvvvLyPkgkispvkeklvtalaenakvlaikGdFDdaq 137
                                               +elg +tv cA++G++++++a a+a++a+++++v++P++ ++  ++k++  l++  +++a+kG++D+++
  lcl|NCBI__GCF_000020025.1:WP_012407541.1 136 RELGFTTVSCASTGNLANSTA-AIAAHAGLDCCVFIPAD-LE--AGKIIGSLIYSPTLMAVKGNYDQVN 200
                                               *********************.*****************.88..9************************ PP

                                 TIGR00260 138 dlvkeifedkeklklnsvNsinparieaqktyafeiveqlgkespdkvvvpvp.sgnfgailkGflekk 205
                                               +l+ e++ +   ++++++N++ p++ e++kt+ fe++eqlg+e pd+vv p++ +++f++i+kGf+e+ 
  lcl|NCBI__GCF_000020025.1:WP_012407541.1 201 RLCSEVA-NTHGWGFVNINLR-PYYSEGSKTLGFEVAEQLGWELPDHVVAPLAsGSLFTKIYKGFQEFI 267
                                               *******.889********88.*******************************8889************ PP

                                 TIGR00260 206 elg....lpieklaiaaegaadivrrflksgdlepkedkeTlstAmdignpsnveralelarrslgnle 270
                                               e+g    + +++++++aeg+++i+++f++++d+ +  +++T++++++ignp++  +a+ela++++gn+e
  lcl|NCBI__GCF_000020025.1:WP_012407541.1 268 EVGlvedKKVRFSGAQAEGCSPIAQAFKEGRDFIKPVKPNTIAKSLAIGNPADGIYAVELAQKTGGNIE 336
                                               *****9999************************************************************ PP

                                 TIGR00260 271 dlkesvsdeeileaikklaeeegyllephtavavaalkklvekgvsatadpaKFeevvealtgnklkdp 339
                                               ++    +d+ei++ ik+lae+eg+++e++++++va+lkklve+g   ++dp+  e++v ++tgn+lk++
  lcl|NCBI__GCF_000020025.1:WP_012407541.1 337 SV----NDAEIIDGIKLLAETEGIFTETAGGTTVAVLKKLVEAG---KIDPD--ETTVIYITGNGLKTQ 396
                                               **....**************************************...*****..*************98 PP

                                 TIGR00260 340 e 340
                                               e
  lcl|NCBI__GCF_000020025.1:WP_012407541.1 397 E 397
                                               7 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (340 nodes)
Target sequences:                          1  (436 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 10.59
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis