Alignments for a candidate for ilvC in Nostoc punctiforme ATCC 29133; PCC 73102

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvC in Nostoc punctiforme ATCC 29133; PCC 73102

Align ketol-acid reductoisomerase (NAD+) (EC 1.1.1.382); ketol-acid reductoisomerase (NADP+) (EC 1.1.1.86) (characterized)
to candidate WP_012409985.1 NPUN_RS18295 ketol-acid reductoisomerase

Query= BRENDA::C8WR67
         (344 letters)



>NCBI__GCF_000020025.1:WP_012409985.1
          Length = 331

 Score =  447 bits (1149), Expect = e-130
 Identities = 220/329 (66%), Positives = 267/329 (81%), Gaps = 1/329 (0%)

Query: 1   MEKIYYDADISIQPLADKRIAVIGYGSQGHAHAQNLRDSGFDVVIGLRPGS-SWAKAEAD 59
           M ++YYD D ++  LA K IA+IGYGSQGHAHA NL+DSG +V++GL PGS S AKAEA 
Sbjct: 1   MARMYYDEDANLDLLAGKTIAIIGYGSQGHAHALNLKDSGLNVIVGLYPGSKSVAKAEAA 60

Query: 60  GFRVMAVGEAVEESDVIMILLPDERQPAVYEREIRPYLTAGKALAFAHGFNIHFSQIQPP 119
           G  V +V +A   +D IMILLPDE Q  +Y+ EI P L  G  LAFAHGFNIHF Q+ PP
Sbjct: 61  GLTVKSVADAANAADFIMILLPDEVQKTIYKNEIEPNLEEGNVLAFAHGFNIHFGQVVPP 120

Query: 120 KDVDVFMVAPKGPGHLVRRVYEAGGGVPALIAVHQDASGQAKDLALAYARGIGAGRAGIL 179
            +VDV MVAPKGPGHLVRR YE G GVPAL AV+QDASGQA+D A++YA+GIG  RAG+L
Sbjct: 121 ANVDVVMVAPKGPGHLVRRTYEGGEGVPALFAVYQDASGQARDRAMSYAKGIGGTRAGVL 180

Query: 180 TTTFREETETDLFGEQAVLCGGLSALIKAGFETLVEAGYQPEIAYFECLHEMKLIVDLIY 239
            TTFREETETDLFGEQAVLCGGLSALIKAGFETLVEAGYQPE+AYFECLHE+KLIVDL+ 
Sbjct: 181 ETTFREETETDLFGEQAVLCGGLSALIKAGFETLVEAGYQPELAYFECLHEVKLIVDLVV 240

Query: 240 EGGLEYMRYSISDTAQWGDFTSGPRIINEETKKEMRRILADIQSGAFAKSWILENQANRP 299
           EGGL  MR SIS+TA++GD+T GPRI+ ++TK EM++IL++IQSG FA+ ++LENQ+ +P
Sbjct: 241 EGGLAKMRDSISNTAEYGDYTRGPRIVTQQTKAEMQKILSEIQSGQFAREFVLENQSGKP 300

Query: 300 MFNAINRRELEHPIEVVGRKLRSMMPFIK 328
            F A+ R+E EH IE VG+ LR+M  ++K
Sbjct: 301 GFTAMRRKESEHKIEEVGKDLRAMFSWLK 329


Lambda     K      H
   0.320    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 375
Number of extensions: 10
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 344
Length of database: 331
Length adjustment: 28
Effective length of query: 316
Effective length of database: 303
Effective search space:    95748
Effective search space used:    95748
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate WP_012409985.1 NPUN_RS18295 (ketol-acid reductoisomerase)
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00465.hmm
# target sequence database:        /tmp/gapView.4436.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00465  [M=314]
Accession:   TIGR00465
Description: ilvC: ketol-acid reductoisomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
     2e-153  495.9   0.5   2.3e-153  495.7   0.5    1.0  1  lcl|NCBI__GCF_000020025.1:WP_012409985.1  NPUN_RS18295 ketol-acid reductoi


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000020025.1:WP_012409985.1  NPUN_RS18295 ketol-acid reductoisomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  495.7   0.5  2.3e-153  2.3e-153       1     313 [.      15     328 ..      15     329 .. 0.99

  Alignments for each domain:
  == domain 1  score: 495.7 bits;  conditional E-value: 2.3e-153
                                 TIGR00465   1 lkgkkvaiiGyGsqGeaqalnlrdsglnvivglrkeaaswkkAeedGfkvltveeaikkadlimiLlpD 69 
                                               l+gk++aiiGyGsqG+a+alnl+dsglnvivgl+++++s++kAe  G+ v++v++a+++ad+imiLlpD
  lcl|NCBI__GCF_000020025.1:WP_012409985.1  15 LAGKTIAIIGYGSQGHAHALNLKDSGLNVIVGLYPGSKSVAKAEAAGLTVKSVADAANAADFIMILLPD 83 
                                               689****************************************************************** PP

                                 TIGR00465  70 evqkevyeaeikpllkegkallfsHGfnivfkqivipkdvdvvlvAPKgpGalvReeykegrGvpsliA 138
                                               evqk+ y++ei+p+l+eg++l f+HGfni+f q+v+p++vdvv+vAPKgpG+lvR++y+ g+Gvp+l+A
  lcl|NCBI__GCF_000020025.1:WP_012409985.1  84 EVQKTIYKNEIEPNLEEGNVLAFAHGFNIHFGQVVPPANVDVVMVAPKGPGHLVRRTYEGGEGVPALFA 152
                                               ********************************************************************* PP

                                 TIGR00465 139 veqdvtgeakeiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLveaGyqp 207
                                               v+qd++g+a++ A++yAk+iGg+ragvlettF+eE+e+DLfGEqavLcGgl+alika+f+tLveaGyqp
  lcl|NCBI__GCF_000020025.1:WP_012409985.1 153 VYQDASGQARDRAMSYAKGIGGTRAGVLETTFREETETDLFGEQAVLCGGLSALIKAGFETLVEAGYQP 221
                                               ********************************************************************* PP

                                 TIGR00465 208 elAyfeivhelklivdllkekGlelmrdavsntAklgalelr.eilkeelkkemqkilkeiqnGefake 275
                                               elAyfe++he+klivdl+ e+Gl++mrd++sntA++g+++++ +i+++++k+emqkil eiq+G+fa+e
  lcl|NCBI__GCF_000020025.1:WP_012409985.1 222 ELAYFECLHEVKLIVDLVVEGGLAKMRDSISNTAEYGDYTRGpRIVTQQTKAEMQKILSEIQSGQFARE 290
                                               ********************************************************************* PP

                                 TIGR00465 276 walekeagkpafeearkkekeqeiekvGkelralvkae 313
                                               ++le++ gkp f+++r+ke+e++ie+vGk+lra++++ 
  lcl|NCBI__GCF_000020025.1:WP_012409985.1 291 FVLENQSGKPGFTAMRRKESEHKIEEVGKDLRAMFSWL 328
                                               ***********************************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (314 nodes)
Target sequences:                          1  (331 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 9.17
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis