Alignments for a candidate for ilvI in Nostoc punctiforme ATCC 29133; PCC 73102

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Nostoc punctiforme ATCC 29133; PCC 73102

Align acetolactate synthase (subunit 2/2) (EC 2.2.1.6) (characterized)
to candidate WP_012408685.1 NPUN_RS10365 thiamine pyrophosphate-binding protein

Query= BRENDA::P9WG41
         (618 letters)



>NCBI__GCF_000020025.1:WP_012408685.1
          Length = 568

 Score =  172 bits (437), Expect = 3e-47
 Identities = 158/565 (27%), Positives = 242/565 (42%), Gaps = 32/565 (5%)

Query: 39  TGAQAVIRSLEELGVDVIFGIPGGAVLPVYDPLFDSKKLRHVLVRHEQGAGHAASGYAHV 98
           TG  A++      G+  +FG PG +     D + +   L+++L   E  A  AA GYA  
Sbjct: 5   TGRFAILEQFLADGIHYMFGNPGTSEEGFLDAIREYPDLKYILTLQESVAVMAADGYARA 64

Query: 99  TGRVGVCMATSGPGATNLVTPLADAQMDSIPVVAITGQVGRGLIGTDAFQEADISGITMP 158
           T +  +    S PG  N +  L  A     P+V I G  G      DA    D+     P
Sbjct: 65  TKKPALVQIHSTPGLGNAIGALYQAYRGHSPLVVIGGDAGIKYQAMDAQMAGDLVAFAEP 124

Query: 159 ITKHNFLVRSGDDIPRVLAEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPRME--LPG 216
           +TK + +V     + RVL  A  IAA+   G V V +P D+L         P +E   P 
Sbjct: 125 VTKWSTMVMEPSSLLRVLRRAIKIAATPPMGPVYVCLPVDILDA-------PAVEEVRPT 177

Query: 217 YKPNTK--PHSRQVREAAKLIAAARKPVLYVGGGVIRGEATEQLRELAELTGIPVVTTLM 274
             P+TK  P    ++ AA ++A+A KP+++VG GV    A ++L  +AEL G  V     
Sbjct: 178 SIPSTKVLPDEELLKTAATMLASAHKPMIFVGDGVAYSSAQDELTRVAELLGAEVWEVDA 237

Query: 275 ARGAFPDSHRQNLGMPGMHGTVAAVAALQRSDLLIALGTRFDDRVTGKLDS-FAPEAKVI 333
                  +H    GM G      +     R D+ +  GT     V  +L + FAP AKVI
Sbjct: 238 GELNMSHAHPLYQGMTGHMFGYQSRPITNRGDVNLVCGTYLLPEVFPELGNIFAPGAKVI 297

Query: 334 HADIDPAEIGKNRHADVPIVGDVKAVITELIAMLRHHHIPGTIEMADWWAYLNGVRKTYP 393
           H D++  EI KN   D+ +V D K  +  L  +L         E A          K   
Sbjct: 298 HIDLNAYEIAKNHSVDLGLVSDPKLTLAHLAKVLSAVMTSEQKETAKTRTADIAQAKEEN 357

Query: 394 LSYGPQSD---GSLSPEYVIEKLGEIAGPDAVFVAGVGQHQMWAAQFIRY---EKPRSWL 447
                Q+D       P +      E+A      V    +    +    RY    KP  + 
Sbjct: 358 HKRELQADRENWDAVPLHFSRFAAELAKQLPEDVIIFDEALTCSPDLTRYLPPTKPGHYF 417

Query: 448 NSGGLGTMGFAIPAAMGAKIALPGTEVWAIDGDGCFQMTNQELATCAVEGIPVKVALINN 507
            S G G++G  IP  +GAK+A P T V    GDG    T Q L + A   I  K  + NN
Sbjct: 418 LSRG-GSLGVGIPGTIGAKLANPDTTVIGFTGDGAAMYTIQALWSAARHNIDAKFVICNN 476

Query: 508 GNLGMVRQWQSLFYAERYSQTDLATHSHRIP--------DFVKLAEALGCVGLRCEREED 559
            +  +++     ++ ER    D+  H + +         +FV++A ++G  G+R E+ ++
Sbjct: 477 HSYRLLQVNIQAYWQER----DIPQHEYPLSFDLSKPEINFVEIARSMGVEGVRLEKAQE 532

Query: 560 VVDVINQARAINDCPVVIDFIVGAD 584
           +   + QA A +  P +ID ++ A+
Sbjct: 533 IAPALEQALA-HKGPFLIDVVLEAN 556


Lambda     K      H
   0.319    0.136    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 894
Number of extensions: 43
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 618
Length of database: 568
Length adjustment: 37
Effective length of query: 581
Effective length of database: 531
Effective search space:   308511
Effective search space used:   308511
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis