Align dihydroxyacid dehydratase (EC 4.2.1.9) (characterized)
to candidate WP_012401602.1 BPHY_RS11300 dihydroxy-acid dehydratase
Query= metacyc::MONOMER-18815 (557 letters) >NCBI__GCF_000020045.1:WP_012401602.1 Length = 557 Score = 1006 bits (2601), Expect = 0.0 Identities = 494/556 (88%), Positives = 533/556 (95%) Query: 1 MAFNKRSQNITQGVARSPNRSMYYALGYKKEDFDKPMVGIANGHSTITPCNAGLQRLADA 60 M +N+RS+NITQGVARSPNRSMYYALGY+K DFDKPM+GIANGHSTITPCNAGLQRLADA Sbjct: 1 MPYNRRSKNITQGVARSPNRSMYYALGYQKADFDKPMIGIANGHSTITPCNAGLQRLADA 60 Query: 61 AIDAIKASDANPQVFGTPTISDGMSMGTEGMKYSLISREVIADCIETAAQGQWMDGVVVI 120 A++A+K SDANPQ FGTPTISDGMSMGTEGMKYSL+SREVIADCIET QGQWMDGVVVI Sbjct: 61 AVEAVKRSDANPQTFGTPTISDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVI 120 Query: 121 GGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLTIVSSFEAVGEFTAGRMSQED 180 GGCDKNMPGGMI LAR NVPGIYVYGGTIKPGNWKG DLTIVSSFEAVGEFTAGRMSQED Sbjct: 121 GGCDKNMPGGMIGLARMNVPGIYVYGGTIKPGNWKGTDLTIVSSFEAVGEFTAGRMSQED 180 Query: 181 FEGVEKNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV 240 FEGVE+NACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV Sbjct: 181 FEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV 240 Query: 241 EAIKQDIKPRDIITRKSIENAVALIMATGGSTNAVLHYLAIAHAAEVEWTIDDFERIRRK 300 EA+KQD+KPRDIIT+KSIENAVALIMATGGSTNAVLHYLAIAHAAEV+W+I+DFER+R+K Sbjct: 241 EAVKQDLKPRDIITKKSIENAVALIMATGGSTNAVLHYLAIAHAAEVDWSIEDFERMRKK 300 Query: 301 VPVICNLKPSGQYVATDLHKAGGIPQVMKILLKAGMLHGDCLTITGRTLAEELENVPDTP 360 VPVIC+LKPSG+YVATDLHKAGGIPQV+K+LL AG+LHGDC+TITG+T+AEEL++VP P Sbjct: 301 VPVICDLKPSGKYVATDLHKAGGIPQVLKLLLDAGLLHGDCITITGKTIAEELKDVPAKP 360 Query: 361 RADQDVILPIEKALYAEGHLAILKGNLAEEGAVAKITGLKNPVITGPARVFEDEQSAMEA 420 RADQ VI PI+KALY+EGHLAILKGNLAE+GAVAKITGLKNPVITGPARVF+DEQSAMEA Sbjct: 361 RADQKVIYPIDKALYSEGHLAILKGNLAEDGAVAKITGLKNPVITGPARVFDDEQSAMEA 420 Query: 421 ILADKINAGDILVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV 480 ILAD+I AGD++VLRYLGP+GGPGMPEMLAPTSAIIGKGLGESVG ITDGRFSGGTWGMV Sbjct: 421 ILADQIKAGDVVVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMV 480 Query: 481 VGHVAPEAYVGGTIALVQEGDSITIDAHKLLLQLNVADEELARRRANWKQPAPRYTRGVL 540 VGHVAPEA+VGGTIALVQEGDSITIDAHKLLLQLNV D EL RRRA WKQPAPRYTRGVL Sbjct: 481 VGHVAPEAFVGGTIALVQEGDSITIDAHKLLLQLNVDDGELQRRRAAWKQPAPRYTRGVL 540 Query: 541 AKFSKLASTASKGAVT 556 AKF+ LA A+KGAVT Sbjct: 541 AKFAALAKPANKGAVT 556 Lambda K H 0.316 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1214 Number of extensions: 40 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 557 Length of database: 557 Length adjustment: 36 Effective length of query: 521 Effective length of database: 521 Effective search space: 271441 Effective search space used: 271441 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)
Align candidate WP_012401602.1 BPHY_RS11300 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.8834.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.4e-226 739.2 3.5 1.6e-226 739.0 3.5 1.0 1 lcl|NCBI__GCF_000020045.1:WP_012401602.1 BPHY_RS11300 dihydroxy-acid dehy Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000020045.1:WP_012401602.1 BPHY_RS11300 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 739.0 3.5 1.6e-226 1.6e-226 1 541 [. 18 556 .. 18 557 .] 0.99 Alignments for each domain: == domain 1 score: 739.0 bits; conditional E-value: 1.6e-226 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiam 69 ++r++++a+G++++d++kP+i+++n++++i+P++ l+ la+++ e+++++ + ++ f+t ++sDG++m lcl|NCBI__GCF_000020045.1:WP_012401602.1 18 PNRSMYYALGYQKADFDKPMIGIANGHSTITPCNAGLQRLADAAVEAVKRSDANPQTFGTPTISDGMSM 86 68******************************************************************* PP TIGR00110 70 gheGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktk 138 g+eGmkysL+sre+iaD +et v+++++D++vvi+ CDk++PG +++ +r+n+P i v+GG++++g+ k lcl|NCBI__GCF_000020045.1:WP_012401602.1 87 GTEGMKYSLVSREVIADCIETCVQGQWMDGVVVIGGCDKNMPGGMIGLARMNVPGIYVYGGTIKPGNWK 155 ********************************************************************* PP TIGR00110 139 lsekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstlla 207 + ++++v+ feavge +ag++s+e+ e +e++acP++gsC+G++tan+m++ +ealG+sl +sst++ lcl|NCBI__GCF_000020045.1:WP_012401602.1 156 -GTDLTIVSSFEAVGEFTAGRMSQEDFEGVEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMAN 223 .9******************************************************************* PP TIGR00110 208 tsaekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklsld 276 ++ek ++a +s++ +ve+vk+++kPrdi+tk+++ena++l++a+GGstn+vLh laia+ a+v+ s++ lcl|NCBI__GCF_000020045.1:WP_012401602.1 224 PDQEKVDSAAESARVLVEAVKQDLKPRDIITKKSIENAVALIMATGGSTNAVLHYLAIAHAAEVDWSIE 292 ********************************************************************* PP TIGR00110 277 dfdrlsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlr. 344 df+r+++kvP++++lkPsgk+v +dlh+aGG+++vlk l +gllh d++t+tGkt+ae+l++v+ ++ lcl|NCBI__GCF_000020045.1:WP_012401602.1 293 DFERMRKKVPVICDLKPSGKYVATDLHKAGGIPQVLKLLLDAGLLHGDCITITGKTIAEELKDVPAKPr 361 ****************************************************************99864 PP TIGR00110 345 vdqdvirsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvke 413 +dq+vi ++d+++++eg+la+LkGnlae+Gav+ki+g ++ +++Gpa+vf++e+ a+eail+ ++k+ lcl|NCBI__GCF_000020045.1:WP_012401602.1 362 ADQKVIYPIDKALYSEGHLAILKGNLAEDGAVAKITGLKN--PVITGPARVFDDEQSAMEAILADQIKA 428 7999************************************..999************************ PP TIGR00110 414 GdvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialv 482 Gdvvv+ry GP+GgPGm+emLaPtsa++g GLg++v+LitDGrfsGgt G+++Ghv+Pea +gG+ialv lcl|NCBI__GCF_000020045.1:WP_012401602.1 429 GDVVVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMVVGHVAPEAFVGGTIALV 497 ********************************************************************* PP TIGR00110 483 edGDkikiDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavl 541 ++GD i+iD+++ l+l+v++ el++rra++k++ +r+++g+Lak+a l++ a+kGav+ lcl|NCBI__GCF_000020045.1:WP_012401602.1 498 QEGDSITIDAHKLLLQLNVDDGELQRRRAAWKQPAPRYTRGVLAKFAALAKPANKGAVT 556 *********************************************************96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (557 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.02s 00:00:00.04 Elapsed: 00:00:00.03 # Mc/sec: 7.91 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory