Align glutamyl-tRNAGln amidotransferase subunit B (EC 6.3.5.7) (characterized)
to candidate WP_012467175.1 CLIM_RS11485 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit B
Query= metacyc::MONOMER-13956 (476 letters) >NCBI__GCF_000020465.1:WP_012467175.1 Length = 475 Score = 454 bits (1167), Expect = e-132 Identities = 231/470 (49%), Positives = 314/470 (66%), Gaps = 2/470 (0%) Query: 1 MNFETVIGLEVHVELKTKSKIFSSSPTPFGAEANTQTSVIDLGYPGVLPVLNKEAVEFAM 60 M +E V+GLEVH +L T SK F T FG ANT + L PG LPVLN+ VE A+ Sbjct: 1 MKYELVVGLEVHCQLNTNSKAFCGCSTRFGNPANTNVCPVCLALPGALPVLNRRVVEDAV 60 Query: 61 KAAMALNCEIATDTKFDRKNYFYPDNPKAYQISQFDKPIGENGWIEIEVGGKTKRIGITR 120 K +A C IA + RKNYFYPD PK YQISQF++PI G + I+VG + I + R Sbjct: 61 KLGLATGCSIAPSSILARKNYFYPDLPKGYQISQFEEPICTEGMLRIDVGDGCRDIRLIR 120 Query: 121 LHLEEDAGKLTHTGDGYSLVDFNRQGTPLVEIVSEPDIRTPEEAYAYLEKLKSIIQYTGV 180 +H+EEDAGK H + +D NR G PL+EIVS PDIRT EA AYL+K++ I++Y G+ Sbjct: 121 IHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDIRTSREASAYLQKMRQIVKYLGI 180 Query: 181 SDCKMEEGSLRCDANISLRPIGQEEFGTKTELKNLNSFAFVQKGLEHEEKRQEQVLLSGF 240 SD MEEGSLRCDAN+S+R G EE+GT+TE+KN+NSF V+K +E+E +R ++ G Sbjct: 181 SDGNMEEGSLRCDANVSVRLFGAEEYGTRTEIKNMNSFKNVEKAIEYEMQRHIDIIDGGG 240 Query: 241 FIQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDDEWKERVKASIPELPDER 300 I QETR +D +T MR KE + DYRYFP+PDLV + +D E ER++ +PE P++R Sbjct: 241 TIVQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDQEMLERIRLELPEFPEDR 300 Query: 301 RKRYIEELGFAAYDAMVLTLTKEMADFFEETVQKGAEAKQASNWLMGEVSAYLNAEQKEL 360 +R++ E G YDA VLT+ +E+AD+FE T+ +AK ASNW+MGEV L + ++ Sbjct: 301 ERRFVSEYGIPPYDAGVLTVEREIADYFERTLTVCGDAKAASNWVMGEVMRTLKEKYLDI 360 Query: 361 ADVALTPEGLAGMIKLIEKGTISSKIAKKVFKELIEKG-GDAEKIVKEKGLVQISDEGVL 419 + A++PE L G+I LI KG IS+ IAK+VF EL++ G AE+IV +GL Q+SD G + Sbjct: 361 KEFAISPERLGGLIGLIGKGAISNTIAKQVF-ELMQTGEASAEEIVDREGLAQVSDTGAI 419 Query: 420 LKLVTEALDNNPQSIEDFKNGKDRAIGFLVGQIMKASKGQANPPMVNKIL 469 + V E L+ NP+ + D++ GK + GF VGQ M KG+ANP +VN +L Sbjct: 420 ERTVDEILEANPKQLADYREGKTKLFGFFVGQCMARMKGKANPQVVNDVL 469 Lambda K H 0.314 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 606 Number of extensions: 19 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 476 Length of database: 475 Length adjustment: 33 Effective length of query: 443 Effective length of database: 442 Effective search space: 195806 Effective search space used: 195806 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 51 (24.3 bits)
Align candidate WP_012467175.1 CLIM_RS11485 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit B)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00133.hmm # target sequence database: /tmp/gapView.9415.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00133 [M=481] Accession: TIGR00133 Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-179 583.8 0.0 1.3e-179 583.7 0.0 1.0 1 lcl|NCBI__GCF_000020465.1:WP_012467175.1 CLIM_RS11485 Asp-tRNA(Asn)/Glu-t Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000020465.1:WP_012467175.1 CLIM_RS11485 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit B # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 583.7 0.0 1.3e-179 1.3e-179 2 480 .. 1 473 [. 1 474 [. 0.99 Alignments for each domain: == domain 1 score: 583.7 bits; conditional E-value: 1.3e-179 TIGR00133 2 ieyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalns 70 ++yelv+GlEvH+qlnt+sK Fc cs+++ + + Ntnvcpvcl+lPGalPvlN+++v+ A+kl+la+++ lcl|NCBI__GCF_000020465.1:WP_012467175.1 1 MKYELVVGLEVHCQLNTNSKAFCGCSTRFGN-PANTNVCPVCLALPGALPVLNRRVVEDAVKLGLATGC 68 68*****************************.99*********************************** PP TIGR00133 71 kivsevsvFdRKhYfYpDlPkgyqitqqdlPiaedGkleieleekekeigierlhlEeDtgksqykesd 139 +++ s+ +RK+YfYpDlPkgyqi+q++ Pi+++G+l+i++ ++ + i++ r+h+EeD+gks + d lcl|NCBI__GCF_000020465.1:WP_012467175.1 69 S-IAPSSILARKNYFYPDLPKGYQISQFEEPICTEGMLRIDVGDGCRDIRLIRIHIEEDAGKSIHDIGD 136 *.668************************************************************9776 PP TIGR00133 140 kdkqslvDfNRsgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlkG 208 +++D NR+gvPLlEiV+ Pd++++ ea a+l+k+rqi++yl+isdg++eeGs+R+D+Nvs+rl G lcl|NCBI__GCF_000020465.1:WP_012467175.1 137 ---DTYIDVNRCGVPLLEIVSYPDIRTSREASAYLQKMRQIVKYLGISDGNMEEGSLRCDANVSVRLFG 202 ...69**************************************************************** PP TIGR00133 209 qekegtrvEiKNlnslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRYf 277 e+ gtr EiKN+ns+k++ekaieyE++R ++++ g +++qetr +d k t s+R Ke ++DYRYf lcl|NCBI__GCF_000020465.1:WP_012467175.1 203 AEEYGTRTEIKNMNSFKNVEKAIEYEMQRHIDIIDGGGTIVQETRLWDADKGETRSMRGKEFAHDYRYF 271 ********************************************************************* PP TIGR00133 278 pePdlppieideevvkekveeklpelPeakrirlkkeyglseedakvlvsdlelldafeevvklikepk 346 p+Pdl+p+ +d+e++++ ++ +lpe+Pe + r+++eyg+ +da vl+ ++e++d+fe ++ +++k lcl|NCBI__GCF_000020465.1:WP_012467175.1 272 PDPDLVPVLVDQEMLER-IRLELPEFPEDRERRFVSEYGIPPYDAGVLTVEREIADYFERTLTVCGDAK 339 *************9999.*************************************************** PP TIGR00133 347 lavnWileellgeLnkkkislaeallkpeelaeliklikegkisqksakelleellenkkdpkkliekl 415 +a nW++ e++++L++k ++++e++++pe l li li +g is+++ak+++e + + + +++++++++ lcl|NCBI__GCF_000020465.1:WP_012467175.1 340 AASNWVMGEVMRTLKEKYLDIKEFAISPERLGGLIGLIGKGAISNTIAKQVFELMQTGEASAEEIVDRE 408 ********************************************************************* PP TIGR00133 416 gliqisdekelvkiveevikenpkevekyksgkekalkflvGqvmkktkgradpkevekllkell 480 gl q+sd+ ++ + v+e+++ npk++ +y++gk k+++f+vGq m + kg+a+p+ v+ +lk +l lcl|NCBI__GCF_000020465.1:WP_012467175.1 409 GLAQVSDTGAIERTVDEILEANPKQLADYREGKTKLFGFFVGQCMARMKGKANPQVVNDVLKSRL 473 **************************************************************998 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (481 nodes) Target sequences: 1 (475 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 9.76 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory