Align aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) (characterized)
to candidate WP_012502576.1 CPAR_RS06800 aspartate--tRNA(Asp/Asn) ligase
Query= reanno::Dino:3609247 (590 letters) >NCBI__GCF_000020505.1:WP_012502576.1 Length = 603 Score = 612 bits (1577), Expect = e-179 Identities = 317/593 (53%), Positives = 412/593 (69%), Gaps = 12/593 (2%) Query: 4 FRTHTCAELTKDAVGQTVRLSGWVHRVRDHGGVLFIDLRDHYGMTQVLCDPDS-PVFSQV 62 FRTH C L +D+ G V+L GWVHR+RDHGG++FIDLRDH G+ Q++ P+S +F Sbjct: 15 FRTHYCGLLNRDSEGAGVKLGGWVHRIRDHGGLIFIDLRDHTGICQLVIQPESGELFRIA 74 Query: 63 EKVRSEWCIRIDGTVKARDESLINPKIPTGEIEVFIRDMEVLGTAEELPLPVFGDQEYPE 122 E + SE I +G+V R + +NP++ +G IEV + +++ A LP PV E Sbjct: 75 EGLHSESVISAEGSVVLRSDETVNPRLASGAIEVVVSAIKIESAAHPLPFPVADYMPTSE 134 Query: 123 ETRLKYRFLDLRRESLHDNIMLRSKVVQSIRKRMWDIDFTEFQTPIITASSPEGARDFLV 182 E RLKYRFLDLRRE LH+NI+ RS++ ++RK + D+DF E QTPI+T+SSPEGARDFLV Sbjct: 135 ELRLKYRFLDLRRERLHENIIFRSQLTAAVRKYLTDLDFIEIQTPILTSSSPEGARDFLV 194 Query: 183 PSRLHPGKFYALPQAPQQFKQLIMVGGFDKYFQIAPCFRDEDPRADRSPTDFYQLDLEMS 242 PSRLHPGKFYALPQAPQQFKQL+MV GF +YFQIAPCFRDED RADRSP +FYQ+D+EMS Sbjct: 195 PSRLHPGKFYALPQAPQQFKQLLMVSGFPRYFQIAPCFRDEDARADRSPGEFYQVDIEMS 254 Query: 243 FVSQQDVFDTIQPVIAGIFEEFGGGRRVDTDWPLISYRDSALWYGTDKPDLRNPIKMQIV 302 FV Q D+F ++ + + E R +P ISY+D YG+DKPDLR PI++Q V Sbjct: 255 FVEQDDLFVILEGMFKHLVETMTTKRIAQYPFPRISYKDVMNRYGSDKPDLRIPIEIQDV 314 Query: 303 SDHFAGSGFAIFAKLLEQEGTEIRAIPAPGGG--SRKFCDRMNKFAQEQGLPGMGYIFWR 360 ++ F SGF +FA +EG ++A+ G G SR F D+ K A+E G G+ YI ++ Sbjct: 315 TELFVNSGFKVFAS-NTKEGCCVKAMVLKGMGNESRLFYDKAEKRARELGSAGLAYIQFK 373 Query: 361 DQGQGMEAAGPLAKNIGPERTEAIRQQLGLQVGDAAFFLGGKPASFEAVAGRARTVIGEE 420 ++ GP+ K + E A+++QLG++ GD FF GK + G R + Sbjct: 374 EEA----PKGPVVKFLKEEEMAALKEQLGIETGDVVFFGAGKWEQTCKIMGGMRNYFADV 429 Query: 421 LGLIDQDRFAFAWIVDFPMYEKDDEG-RIDFSHNPFSMPQGGMAALE-GDPLEVLGYQYD 478 L D+D +F WIVDFPM+E ++E +IDFSHNPFSMPQG M ALE PL++L YQYD Sbjct: 430 FPL-DRDELSFCWIVDFPMFEYNEEDKKIDFSHNPFSMPQGEMEALEQHSPLDILAYQYD 488 Query: 479 LACNGYELVSGAIRNHKLDIMYKAFEIAGYGADEVEKRFGGMVNAFKYGPPPHGGCAAGI 538 + CNG EL SGAIRNH+ DIMYKAFEIAGY DEV+ RFG M+ AFK+G PPHGG A G+ Sbjct: 489 IVCNGIELSSGAIRNHRPDIMYKAFEIAGYPHDEVDARFGHMIEAFKHGAPPHGGIAPGL 548 Query: 539 DRIVMLLADTANIREVIMFPMNQRAEDLMMNAPSEPTGEQLRDLSLRV-IPQE 590 DR+VM+L D NIREVI FPMNQ A+DLMM APSE T +QL++L ++V +P+E Sbjct: 549 DRLVMILRDEQNIREVIAFPMNQSAQDLMMAAPSEVTAQQLKELHIKVELPEE 601 Lambda K H 0.322 0.141 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1051 Number of extensions: 46 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 590 Length of database: 603 Length adjustment: 37 Effective length of query: 553 Effective length of database: 566 Effective search space: 312998 Effective search space used: 312998 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory