Align aspartate kinase (EC 2.7.2.4) (characterized)
to candidate WP_012475797.1 CPHAMN1_RS12085 lysine-sensitive aspartokinase 3
Query= BRENDA::Q57991 (473 letters) >NCBI__GCF_000020545.1:WP_012475797.1 Length = 471 Score = 327 bits (839), Expect = 4e-94 Identities = 197/479 (41%), Positives = 298/479 (62%), Gaps = 30/479 (6%) Query: 4 VMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVEISQQALDVRDIA 63 VMKFGGTS+G+ ++ +IV R+KE + +VV+SA S +TN L++I++ A D R + Sbjct: 3 VMKFGGTSIGTASAMKRAMQIVG-REKEKNIPLVVLSACSGITNRLIQIAEAAGDSR-LD 60 Query: 64 KVGDFIKFIREKHYKAIEEAIKSEEIKEEVKKIIDSRIEELEKVLIGVAYLGELTPKSRD 123 + +RE H + +EE I +++ +K+ +D+ I EL+ ++ GV +GELT +S D Sbjct: 61 EAMRLSGEVREHHLELVEELINDASLRQSLKEKVDAYIGELDMLIQGVDIVGELTARSFD 120 Query: 124 YILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITDNNFGSAR----VKRLEVKERLL 179 SFGE LS+ + S A+R+ G ++ + E +ITD+NF +AR + V+ L Sbjct: 121 TFCSFGELLSTTVFSDAMREAGYDTVWKDIREV-MITDDNFSAARPFEDLCEKNVQNSLR 179 Query: 180 PLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTD 239 PLL++G I V GFIG+T +G TT+GRGGSDY+AAL+G L +D I+IWTDV GV T D Sbjct: 180 PLLEQGSIVVTQGFIGSTRDGKTTTMGRGGSDYTAALLGAWLPSDEIQIWTDVDGVMTCD 239 Query: 240 PRLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLIT 299 PR+VP AR I +++ EA EL+Y GAKVLHP TI PA+++ IP+ V N+ PES+GT+IT Sbjct: 240 PRIVPDARSIRVMTFTEAAELSYLGAKVLHPDTIAPAVKQNIPVYVLNSLCPESKGTVIT 299 Query: 300 NDMEMSD-----SIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQ 354 N+ E + +VK+I+ K +IN M+G G A IF V+V ++S Sbjct: 300 NNPETLEGMSYGGLVKSIAVKKGQCIINFRSNRMLGRHGFMADIFGVFARAGVSVEMVS- 358 Query: 355 GSSETNISLVVSEEDVDKALKALKREFGDFGKKSFLNNNLIRDVSVDKDVCVISVVGAGM 414 +SE ++SL VSE +L++ D K + +V ++ DV ISVVG + Sbjct: 359 -TSEVSVSLTVSES------ASLEQLLDDLRK--------MGEVEIEPDVATISVVGDNL 403 Query: 415 RGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKFIEKTNS 473 R AKG+AG+IF+A+ + NI+MI+QG+SE+N+ FV++ +D+ V+ LH +F S Sbjct: 404 RTAKGMAGRIFSAMKD--VNIRMISQGASEINVGFVVEGEDVFRAVQHLHHEFFSDIES 460 Lambda K H 0.316 0.135 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 521 Number of extensions: 22 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 473 Length of database: 471 Length adjustment: 33 Effective length of query: 440 Effective length of database: 438 Effective search space: 192720 Effective search space used: 192720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 51 (24.3 bits)
Align candidate WP_012475797.1 CPHAMN1_RS12085 (lysine-sensitive aspartokinase 3)
to HMM TIGR00657 (aspartate kinase (EC 2.7.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00657.hmm # target sequence database: /tmp/gapView.13409.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00657 [M=442] Accession: TIGR00657 Description: asp_kinases: aspartate kinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4e-124 401.0 0.8 4.5e-124 400.8 0.8 1.0 1 lcl|NCBI__GCF_000020545.1:WP_012475797.1 CPHAMN1_RS12085 lysine-sensitive Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000020545.1:WP_012475797.1 CPHAMN1_RS12085 lysine-sensitive aspartokinase 3 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 400.8 0.8 4.5e-124 4.5e-124 5 441 .. 3 455 .. 1 456 [. 0.95 Alignments for each domain: == domain 1 score: 400.8 bits; conditional E-value: 4.5e-124 TIGR00657 5 VqKFGGtSvgnverikkvakivkkekekgnqvvVVvSAmagvTdaLvelaekvsseee...keliekir 70 V+KFGGtS+g++ +k++ +iv +ekek+ ++VV+SA +g+T++L+++ae + + + ++l ++r lcl|NCBI__GCF_000020545.1:WP_012475797.1 3 VMKFGGTSIGTASAMKRAMQIVGREKEKN-IPLVVLSACSGITNRLIQIAEAAGDSRLdeaMRLSGEVR 70 9*************************777.99*********************99998776666779** PP TIGR00657 71 ekhlealeela.sqalkeklkallekeleevkk............ereldlilsvGEklSaallaaale 126 e+hle +eel+ +l++ lk+ +++ + e+ r++d+ s+GE lS+++++ a++ lcl|NCBI__GCF_000020545.1:WP_012475797.1 71 EHHLELVEELInDASLRQSLKEKVDAYIGELDMliqgvdivgeltARSFDTFCSFGELLSTTVFSDAMR 139 ***********77788899999999998888888999******************************** PP TIGR00657 127 elgvkavsllgaeagiltdsefgrAk....vleeikterleklleegiivvvaGFiGatekgeittLGR 191 e g + + +++++++td++f+ A+ e+ ++ l +lle+g ivv++GFiG+t++g++tt+GR lcl|NCBI__GCF_000020545.1:WP_012475797.1 140 EAG-YDTVWKDIREVMITDDNFSAARpfedLCEKNVQNSLRPLLEQGSIVVTQGFIGSTRDGKTTTMGR 207 ***.777789****************999888899999******************************* PP TIGR00657 192 GGSDltAallAaalkAdeveiytDVdGiytaDPrivpeArrldeisyeEalELaslGakvLhprtlepa 260 GGSD+tAall+a l de++i+tDVdG++t+DPrivp+Ar + +++ Ea+EL++lGakvLhp+t+ pa lcl|NCBI__GCF_000020545.1:WP_012475797.1 208 GGSDYTAALLGAWLPSDEIQIWTDVDGVMTCDPRIVPDARSIRVMTFTEAAELSYLGAKVLHPDTIAPA 276 ********************************************************************* PP TIGR00657 261 mrakipivvkstfnpeaeGTlivaksk....seeepavkalsldknqalvsvsgttmk..pgilaevfg 323 ++++ip+ v ++ pe++GT+i+++ + ++ vk+++++k q +++ +++ m g++a++fg lcl|NCBI__GCF_000020545.1:WP_012475797.1 277 VKQNIPVYVLNSLCPESKGTVITNNPEtlegMSYGGLVKSIAVKKGQCIINFRSNRMLgrHGFMADIFG 345 *************************9999999999**********************999********* PP TIGR00657 324 alaeakvnvdlilqsssetsisfvvdkedadkakellkkkvkeekaleevevekklalvslvGagmksa 392 a+a+v+v++++ +se s+s++v+++ + ++l ++++++++eve+e ++a++s+vG+++++a lcl|NCBI__GCF_000020545.1:WP_012475797.1 346 VFARAGVSVEMVS--TSEVSVSLTVSESA--SLEQL----LDDLRKMGEVEIEPDVATISVVGDNLRTA 406 ************9..8888999*998764..44555....568999*********************** PP TIGR00657 393 pgvaakifeaLaeeniniemis..sseikisvvvdekdaekavealheklv 441 +g+a++if+a+++ +ni+mis +sei++ +vv+++d+ +av+ lh++++ lcl|NCBI__GCF_000020545.1:WP_012475797.1 407 KGMAGRIFSAMKD--VNIRMISqgASEINVGFVVEGEDVFRAVQHLHHEFF 455 ***********99..**********************************98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (442 nodes) Target sequences: 1 (471 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 12.57 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory